Ocimum acquired the spoligotyping kit through an acquisition of the Biomolecules division of Isogen Lifesciences based in The Netherlands. Since the acquisition in 2006, Ocimum has continued to invest in additional R&D and is in the process of adding several new kits to its portfolio.
As with other products, below is a chronological publication reference to the spoligotyping kit. The earliest publication for this product is dated way back in 1997. Many publications will refer to Isogen as that was the name of our subsidiary. Recently we changed the name back to Ocimum Biosolutions but we continue to find references to Isogen.
With the large number of references of over 400 and from labs across the world, the spoligotyping kit has been validated for over 15 years now giving confidence to researchers across the globe using it.
Updated: March 31 2013
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2013
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Spoligotyping of multidrug-resistant Mycobacterium tuberculosis isolates in Ethiopia
clinical isolates were represented by a unique (non-clustered) spoligotype pattern … these patterns,
which are already recorded in the fourth international spoligotyping database (SpolDB4 …
Modern lineages of Mycobacterium tuberculosis in Addis Ababa, Ethiopia: implications for the tuberculosis control programe
with already existing designations in the international spoligotyping database, SpolDB4 … 5. In this
database, two or more patient isolates sharing identical spoligotype patterns are …
[HTML] Admixed Phylogenetic Distribution of Drug Resistant Mycobacterium tuberculosis in Saudi Arabia
isolates sharing identical MIRU-VNTR types and spoligotypes. … loci based MIRU-VNTR typing
(Genoscreen, Lille, France) and Spoligotyping (Ocimum Biosolutions, Hyderabad, India …
[PDF] Abattoir-based study on the epidemiology of caprine tuberculosis in Ethiopia using conventional and molecular tools
described earlier [28] and according to the spoligotype kit supplier’s instructions
(Ocimum Biosolutions Company, Iisselstein, The Netherlands). …
Molecular typing of mycobacteria isolated from extrapulmonary tuberculosis patients at Debre Birhan Referral Hospital, central Ethiopia
by spoligotyping techniques, as previously described [11,12], and in accordance with the
spoligotype kit supplier instructions (Ocimum Biosolutions Company, IJsselstein …
Endogenous reactivation followed by exogenous re-infection with drug resistant strains, a new challenge for tuberculosis control in Saudi Arabia
Hyderabad, India) and 24 loci based MIRU-VNTR typing (Genoscreen, Lille, France) by using
the commercially available kits and methods described previously. …
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2012
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A Ruettger, J Nieter, A Skrypnyk… – Journal of Clinical …, 2012 – Am Soc Microbiol
… Short-Form Paper 4 reverse line blot hybridization using the Spoligotyping Kit (Ocimum
Biosolutions, Hydera- 79 bad, India) and the present DNA microarray. … ArrayStrip spoligotyping
and the assay’s capability of identifying new spoligotypes and li- 109 neages. …
MI Millán-Lou, H Alonso, P Gavín… – Journal of Clinical …, 2012 – Am Soc Microbiol
… First, all 292 isolates were screened by spoligotyping with a commercially available
kit (Ocimum Biosolutions Ltd., Hyderabad, India) by the method of Kamerbeek et al.
(14). Seventy of the 292 isolates belonged to the Beijing family. …
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GenoType MTBDRsl performance on clinical samples with diverse genetic background
P Miotto, AM Cabibbe, P Mantegani… – European …, 2012 – Eur Respiratory Soc
… Spoligotyping. The amplified direct repeat locus of clinical isolates was investigated using the
Spoligotyping kit (Ocimum Biosolutions, Hyderabad, India) following the manufacturer’s
instructions. Spoligotyping results were entered, in binary format, in the MIRU-VNTRplus web …
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B Varghese, A Hillemann, DR Wijayanti… – Infection, Genetics and …, 2012 – Elsevier
… Of the 151 isolates, 146 with interpretable mutation results were subjected to MIRU-VNTR typing
and spoligotyping. Spoligotyping was performed as previously described (Kamerbeek et al., 1997)
with the commercial kit (Ocimum Biosolutions, Hyderabad, India). …
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O Kisa, G Tarhan, S Gunal, A Albay, R Durmaz… – PloS one, 2012 – dx.plos.org
… Spoligotyping was carried out with a commercial kit (Isogen Bioscience BV, Maarssen, The
Netherlands … tuberculosis H37Rv and M.bovis BCG P3 were used as control for spoligotyping.
Spoligotypes results were converted into octal code and in the SITVIT2 proprietary database …
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Y Pang, Y Zhou, B Zhao, G Liu, G Jiang, H Xia… – PloS one, 2012 – dx.plos.org
… A commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands) was used as
described by … of the direct repeat region of Mycobacterium tuberculosis: implications for
interpretation of spoligotyping data. … 2004) Predominant tuberculosis spoligotypes, Delhi, India. …
PM Parreiras, GI Andrade… – Memórias do Instituto …, 2012 – SciELO Brasil
… The PCR products were hybridised to a membrane (Isogen, The Netherlands) containing 43
oligonucleotides of known spacer sequences by reversed-line blot hybridisation. … Spoligotyping –
Complete spoligotypes were obtained from 61 isolates (Table I). In total, 17 …
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EVALUATION OF 24-LOCUS MIRU-VNTR IN EXTRAPULMONARY SPECIMENS: STUDY FROM A TERTIARY CENTRE IN MUMBAI
V Vadwai, A Shetty, P Supply… – Tuberculosis, 2012 – Elsevier
… Spoligotyping, was performed using a commercial kit (Isogen, Netherlands) as per the … 13/69,
18.8%) spoligo-prototypes, compared to LAM, Haarlem or T spoligotypes represented at … Analysis
of the congruence between MIRU-VNTR and spoligotyping results combined with the …
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V Di Marco, P Mazzone, MT Capucchio… – Journal of Clinical …, 2012 – Am Soc Microbiol
… direct repeat locus were detected by hybridization onto a spoligotyping membrane (Isogen 231 …
M. bovis isolates from both field studies were genotyped by both spoligotyping 342 … Four
spoligotypes were identified: SB0120 (23 isolates), SB0841 (five isolates), SB0833 (two 344 …
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TRANSMISSION OF TUBERCULOSIS WITHIN FAMILY-HOUSEHOLDS
E Augustynowicz-Kopeć, T Jagielski, M Kozińska… – Journal of Infection, 2012 – Elsevier
… Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen …
had different M. tuberculosis isolates, as evidenced by both spoligotyping and MIRU … Assuming
isolates revealing both identical spoligotypes and VNTR profiles are representing the …
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A Deribew, G Abebe, L Apers… – BMC Infectious …, 2012 – biomedcentral.com
… Standard spoligotyping method [12] was done generally as described by Kamerbeek and
colleagues using a commercially available kit (Isogen Bioscience BV … Spoligotypes described
only once (non-clustered) in this study and in the SpolDB4 were designated as …
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A Ayaz, Z Hasan, S Jafri, R Inayat, R Mangi… – International Journal of …, 2012 – Elsevier
… The spoligotypes were compared with the most prevalent MTB subfamilies as identified by the
World Spoligotyping Database SpolDB4.0 of the Pasteur Institute, Guadeloupe. 18. … 3.4. Genotypic
pattern of MTB isolates. Of the 1004 isolates, 987 were spoligotyped. …
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S David, EL Duarte, CQF Leite, JN Ribeiro… – Infection, Genetics and …, 2012 – Elsevier
… patient), were genotyped by spoligotyping according to the manufacturer’s instructions (Isogen
Bioscience BV … Contribution of Spoligotyping to the Characterization of the population structure
of … Update on the Spoligotypes of Mycobacterium tuberculosis complex isolates from the …
ÜO ZEYTİNLİ… – Mikrobiyol Bul, 2012 – mikrobiyolbul.org
… Key words: Mycobacterium tuberculosis; spoligotyping; MIRU-VNTR; molecular epidemiology;
Turkey. GİRİŞ … Spoligotiplendirme Spoligotiplendirme yöntemi, üretici firmanın önerileri
(Isogen Bioscience BV, Hollan- da) dikkate alınarak uygulandı. …
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2011
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W Waters, GE Stevens, MA Schoenbaum… – Veterinary medicine …, 2011 – hindawi.com
… Isolates of M. bovis were identified by a combination of Ziehl-Neelsen acid-fast staining, nucleic
acid probes (AccuProbe, Gen-Probe, San Diego, CA, USA), and spoligotyping (Ocimum
Biosolutions Ltd., Hyderabad, India). Identification of atypical Mycobacteria spp. …
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E Abadia, J Zhang, V Ritacco… – BMC infectious …, 2011 – biomedcentral.com
… of hard to interpret spoligotypes and samples representative of the work flow, so we could evaluate
the benefits of a new platform regarding the classical one on these samples. Most centers had
performed spoligotyping using commercial membranes (Ocimum, Hyderabad, India …
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PJ Freidlin, D Goldblatt, H Kaidar-Shwartz… – … and Quality Assurance: …, 2011 – Springer
… for spoli- gotyping results, standardization and reproducibility depend on the quality of the
commercial spoligotyping kit (Ocimum). … Spoligotyping Pattern … Both PETRA (a MIRU-VNTR lineage
with central asian spoligotypes, CAS) and Beijing clusters have been observed in other …
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S Purwar, S Chaudhari, VM Katoch… – Infection, Genetics and …, 2011 – Elsevier
… commercially available kit 126 (Isogen Biosciences , BV, Maarsen, The Netherlands now Ocimum
Biosolutions) and … Owing to small sample size, the exact prevalence of various 272 spoligotypes
and M … Out of the isolates clustered by spoligotyping, all 280 were unique strains by …
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B Varghese, A Hillemann, DR Wijayanti… – Infection, Genetics and …, 2012 – Elsevier
… Of the 151 isolates, 146 with interpretable mutation results were subjected to MIRU-VNTR typing
and spoligotyping. Spoligotyping was performed as previously described (Kamerbeek et al., 1997)
with the commercial kit (Ocimum Biosolutions, Hyderabad, India). …
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T Oni, R Burke, R Tsekela, N Bangani, R Seldon… – Thorax, 2011 – thorax.highwire.org
… Lowenstein–Jensen slope cultures. The DNA was used to perform spoligotyping
as previously described4 using a commercially available kit from Ocimum Biosolutions
BV, The Netherlands. The hybridisation patterns obtained …
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G Mamo, G Bayleyegn, TS Tessema, M Legesse… – PloS one, 2011 – dx.plos.org
… Spoligotyping. Spoligotyping was performed as previously described by Kamerbeek
and coauthors [22] and according to the spoligotype kit supplier’s instructions (Ocimum
Biosolutions Company, Iisselstein, The Netherlands). …
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POPULATION-BASED INVESTIGATION OF FLUOROQUINOLONES RESISTANT TUBERCULOSIS IN RURAL EASTERN CHINA
Y Hu, B Mathema, W Wang, B Kreiswirth, W Jiang… – Tuberculosis, 2011 – Elsevier
… Spoligotyping was carried out using commercial kits from Isogen Bioscience BV (Maarssen, The
Netherlands). 15 Strain family was determined by comparison of spoligotyping patterns with the
SpoligDB4 database and assigned a spoligotyping international type (SIT). …
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[HTML] THE GUINEA-BISSAU FAMILY OF MYCOBACTERIUM TUBERCULOSIS COMPLEX REVISITED
R Groenheit, S Ghebremichael, J Svensson… – PLoS One, 2011 – dx.plos.org
… All isolates that were subjected to RFLP were also spoligotyped. … Altogether, 414 isolates were
available for spoligotyping, of which 336 isolates were grouped into 42 clusters … Out of 229 isolates,
82 different spoligotypes were obtained, of which 168 (73.4%) were clustered into …
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GENETIC DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS IN MBARARA, SOUTH WESTERN UGANDA
J Bazira, M Matte, BB Asiimwe… – African health sciences, 2011 – ajol.info
… was performed as previously described29 using a commercially available kit (Isogen Bioscience
BV … Spoligotypes that did not match any existing pattern in the SpolDB4 database were … tuberculosis
within the Mbarara, we have used RD analysis and spoligotyping to characterize …
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Y Rafiq, K Jabeen, R Hasan, S Jafri… – Antimicrobial agents …, 2011 – Am Soc Microbiol
… was performed on all FQ-resistant strains using a commercially available kit provided by Isogen
Life Science BV … The spoligotypes were compared with the most prevalent M. tuberculosis
subfamilies, as identified by the world spoligotyping database, SpolDB4.0, of …
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[HTML] CHARACTERISTICS OF PNCA MUTATIONS IN MULTIDRUG-RESISTANT TUBERCULOSIS IN TAIWAN
YC Chiu, SF Huang, KW Yu, YC Lee… – BMC infectious …, 2011 – biomedcentral.com
… Genotyping. All clinical isolates were genotyped by a commercial spoligotyping kit (Isogen
Bioscience BV, Maarssen, Netherlands). The “Beijing strain” was defined as deletion from spacer
1 to spacer 34 in the direct repeat region and the presence of (at least 3) spacers 35-43. …
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JY Feng, WJ Su, YC Chiu, SF Huang, YY Lin… – PloS one, 2011 – dx.plos.org
… All clinical isolates were genotyped using a commercial spoligotyping kit (Isogen Bioscience
BV, Maarssen, Netherlands). The “Beijing strain” was defined as a deletion from spacer 1 to spacer
34 in the direct repeat region and the presence of (at least 3) spacers in 35–43. …
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[HTML] MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS, BUENOS AIRES, ARGENTINA
X Gonzalo, M Ambroggi, E Cordova… – Emerging Infectious …, 2011 – ncbi.nlm.nih.gov
… DNA was extracted from cultures at this UK laboratory; spoligotyping was performed according
to the manufacturer’s instructions (Isogen Life Science, IJsselstein, the Netherlands); and data
were analyzed with SPOTCLUST (http://cgi2.cs.rpi.edu/~bennek/SPOTCLUST.html). …
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CW Chang, MH Wu, PC Chuang… – Infection, Genetics and Evolution, 2011 – Elsevier
… 12 13 Spoligotyping 14 A commercially available kit (Isogen Bioscience BV, Maarssen, The 15
Netherlands) was used as … Even through spoligotyping results 3 revealed the genetic diversities
of MDR M. tuberculosis isolates in Taiwan, 4 identical spoligotypes could not …
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IF Padilla-Campos, JC Segura-Correa… – Tropical and …, 2011 – redalyc.uaemex.mx
… Key words: Bovine tuberculosis; Spoligotypes; M. bovis; Risk factors. … Spoligotyping was carried
out following the protocol described by Kamerbeek et al … sequences from M. tuberculosis and
six spacer sequences for M. bovis BCG were covalently bound (ISOGEN, Maarssen, The …
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[HTML] FINE-TUNING THE SPACE, TIME, AND HOST DISTRIBUTION OF MYCOBACTERIA IN WILDLIFE
C Gortazar, MJ Torres, P Acevedo, J Aznar… – BMC …, 2011 – biomedcentral.com
… Typing. The M. bovis isolates were further characterized by spoligotyping [40]. The amplified
product was detected by hybridization of the biotin-labelled PCR product onto spoligotyping
membrane (Isogen Bioscience BV, Maarssen, The Netherlands). …
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IF Padilla, JC Segura, JC Rodriguez… – Tropical and …, 2011 – ccba.uady.mx
… Key words: Bovine tuberculosis; Spoligotypes; M. bovis; Risk factors. … Spoligotyping was carried
out following the protocol described by Kamerbeek et al … sequences from M. tuberculosis and
six spacer sequences for M. bovis BCG were covalently bound (ISOGEN, Maarssen, The …
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K Faksri, F Drobniewski, V Nikolayevskyy, T Brown… – Tuberculosis, 2011 – Elsevier
… isolated from CSF samples collected from 1996 to 2004 with IS6110 RFLPs (120 RFLP patterns
were available) and spoligotyping results (141 spoligotypes were available … 27 Spoligotyping
was performed according to the manufacturer’s instructions (Isogen Bioscience BV …
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M Alonso, Y Navarro, F Barletta… – Clinical …, 2011 – Wiley Online Library
… Thirty-two MTB strains (18 representatives of the Beijing genotype and 14 representatives of
the non-Beijing genotype, according to their spoligotypes) were grown on … Spoligotyping was
performed in accordance with the manufacturer’s instructions (Isogen, De Meern, the …
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PJ Freidlin, D Goldblatt, H Kaidar-Shwartz… – … and Quality Assurance: …, 2011 – Springer
… 43 spacer spoligotyping was performed according to the manufacturer’s instructions
(Isogen/Ocimun) as described previously. … 27 Spoligotyping Pattern … Both PETRA (a MIRU-VNTR
lineage with central asian spoligotypes, CAS) and Beijing clusters have been observed in other …
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EPIDEMIOLOGICAL ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS STRAINS ISOLATED IN LODZ, POLAND
M Krawczyk, A Brzostek, A Gorna… – … of Tuberculosis and …, 2011 – ingentaconnect.com
… of van Embden et al.16 Spoligotyping was performed as described by Kamerbeek et al., using
the Isogen Life Science kit … Spoligotyping analysis showed that 184 (78.6%) of the strains were
grouped in clusters and 50 (21.4%) strains possessed unique spoligotypes. …
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U Bidovec-Stojkovic, M Zolnir-Dovc… – Respiratory Medicine, 2011 – Elsevier
… 4,9,20 Spoligotyping was performed using a commercially available kit (Isogen Bioscience BV …
identically defined by IS6110 RFLP and 24-locus MIRU-VNTR typing combined with spoligotyping. …
jointly defined by identical 24-locus MIRU-VNTR types and spoligotypes were only …
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Y Du, Y Qi, L Yu, J Lin, S Liu, H Ni… – Research in veterinary …, 2011 – Elsevier
… 2.5. Spoligotyping. Spoligotyping kits and Mini-blotter MN45 were obtained from Isogen
Life Science Co. (Utrecht, the Netherlands). The Streptavidin-ß-peroxidase conjugate was
produced by Roche Applied Science Co. (Penzberg, Germany). …
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Y Hu, B Mathema, W Jiang, B Kreiswirth, W Wang… – PloS one, 2011 – dx.plos.org
… Hybridized DNA was detected using an enhanced chemiluminescene kit from Isogen Bioscience
BV … b Probaiblity that the Spoligotyping pattern belongs to the clades. … N, Bennett KP (2006)
Identifying Mycobacterium tuberculosis complex strain families using spoligotypes. …
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SUBLINEAGES OF BEIJING STRAIN OF MYCOBACTERIUM TUBERCULOSIS IN SRI LANKA
US Rajapaksa… – Indian Journal of Microbiology, 2011 – Springer
… Spoligotyping was carried out according to manufacturer’s guidelines using a
commercially available kit (Isogen, Maarsen MV, The Netherland). Fourteen iso-
lates that confirmed to be Beijing strain were further characterised. …
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S Purwar, S Chaudhari, VM Katoch… – Infection, Genetics and …, 2011 – Elsevier
… 43 spacers was done with the help of a commercially available kit 126 (Isogen Biosciences ,
BV … Owing to small sample size, the exact prevalence of various 272 spoligotypes and M.
tuberculosis … Out of the isolates clustered by spoligotyping, all 280 were unique strains by IS6110 …
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MOLECULAR EPIDEMIOLOGY OF HUMAN AND ANIMAL TUBERCULOSIS IN IBADAN, SOUTHWESTERN NIGERIA
AO Jenkins, SIB Cadmus, EH Venter… – Veterinary …, 2011 – Elsevier
… Spoligotyping167 Spoligotyping confirmed the species identification of 19 M. tuberculosis and
13 M … None of these CAM 173 variant spoligotypes were found in the spolDB4 database and were …
All M. tuberculosis strains 175 from PTB patients that were spoligotyped had identical …
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K Faksri, F Drobniewski, V Nikolayevskyy… – Infection, Genetics and …, 2011 – Elsevier
… Eighty MTB Beijing isolates from CSF samples with IS6110 RFLPs (69 RFLP patterns were
available) and spoligotyping results (all 80 spoligotypes were available … Spoligotyping was
performed according to the manufacturer s instructions (Isogen Bioscience BV …
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[PDF] 骨结核临床分离菌株基因分型的特点
李文胜， 刘毅， 孙勇， 田苗， 刘忠泉… – 中华临床医师杂志 ( …, 2011 – clinicmed.net
… TM 琼脂糖购自赛百盛基因技 术有限公司。 DNA Ladder Marker 购自TaＫaRa 公司。
凝胶成像仪购自FOTO DＹNE 公司。 Spoligotyping 试 剂盒以及Miniblotter MN４５为荷兰
Isogen Biosｃienｃe 公司产品。 链霉亲和素为Sigam 公司产品。 化学发光 …
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MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS IN THE UNITED ARAB EMIRATES
A Saedi… – 2011 – qmro.qmul.ac.uk
… 130 3.2.11 Identification of the UAE isolates by spoligotyping….. 132 3.2.12 Comparison between
MIRU-typed and spoligotyped families in association with katG463/gyrA95 groupings ….. …
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[HTML] MYCOBACTERIUM BOVIS IN SWINE: SPOLIGOTYPING OF ISOLATES FROM ARGENTINA
S Barandiaran, EV Moras, A Adri& – Veterinary Medicine …, 2011 – hindawi.com
… Spoligotyping [14] was carried out by using the spoligotyping kit (Isogen Biosolutions BV, Ocimun …
Guerin (BCG) (ATCC 27289) were included as reference strains in each spoligotyping experiment …
isolates were defined as two or more M. bovis strains with identical spoligotypes. …
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[HTML] SPOLIGOTYPING OF CLINICAL MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM THE STATE OF MINAS GERAIS, BRAZIL
SS Miranda, WS Carvalho, PN Suffys… – Memórias do Instituto …, 2011 – SciELO Brasil
… was resuspended in the same TE buffer, stored at -20ºC and subjected to spoligotyping using
a previously published protocol (Kamerbeek et al. 1997) and a commercially available kit (Isogen
Life Science BV, Utrecht, The Netherlands). The hybridised spoligotypes in binary …
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EVALUATION OF SPOLIGOTYPING AND MIRU-VNTR FOR MYCOBACTERIUM BOVIS IN XINJIANG, CHINA
Z Sun, R Cao, M Tian, X Zhang, X Zhang… – Research in Veterinary …, 2011 – Elsevier
… Additionally, two new spoligotypes (SB1903 and SB1904) and special repeat numbers of 3 loci
(ETR … Spoligotyping was firstly analyzed and interpreted in octal codes (Dale et al., 2001) following
the instruction (http://www.mbovis.org) with a commercial kit (Isogen Bioscience BV …
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J Bazira, BB Asiimwe, ML Joloba… – BMC infectious …, 2011 – biomedcentral.com
… Spoligotyping. Standard spoligotyping was performed as previously described [29] using a
commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands). … Spoligotyping.
All the isolates had spoligotypes characteristic of M. tuberculosis. …
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I Cerezo, Y Jiménez, J Hernandez, T Zozio… – Infection, Genetics and …, 2011 – Elsevier
… Kamerbeek et al., 1997) according to the manufacturer s instructions (Isogen Bioscience, the …
Spoligotype International Type (SIT) and MIRU International Type (MIT) designate spoligotypes
and MIRU … was used to build minimum spanning trees based on spoligotyping and MIRU …
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[PDF] from digitaljournals.org
V Lisdawati, I Parwati… – Bulletin of Health …, 2011 – indonesia.digitaljournals.org
… PCR, denaturasi menggunakan thermocycler selama 10 menit pada suhu 99,9°C. Kemudian
pada mini blotter MN45 yang telah diletakkan membran spoligotyping kit (IM9702, Isogen
Biosolution, BV) berlabel 43 DNA spacer Mtb complex segera hibridisasikan DNA hasil PCR. …
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[PDF] INFECTION OF EURASIAN BADGERS (MELES MELES) WITH MYCOBACTERIUM BOVIS
A Balseiro, O Rodríguez, P González-Quirós… – prevalence, 2011 – uclm.es
… al., 2009). 125 MTC isolates were characterised hybridisation of biotin-labelled PCR
products 126 onto a spoligotyping membrane (Isogen Bioscience BV), followed by
spoligotyping 127 (Kamerbeek et al., 1997). Results were …
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HL Hsu, CC Lai, MC Yu, FL Yu, JC Lee… – European journal of …, 2011 – Springer
… commercially available spoligotyping kit (Isogen Bioscience BV, Maarssen, The Netherlands)
was used as described by the manufacturer. The fourth international spoligotyping database
(SpolDB4) database was used to assign genotype families to the spoligotypes obtained [14 …
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MOLECULAR TYPING OF MYCOBACTERIUM TUBERCULOSIS CIRCULATED IN MOSCOW, RUSSIAN FEDERATION
MV Afanas’ ev, LN Ikryannikova, EN Il’ina… – European Journal of …, 2011 – Springer
… spacers probes from the spoligotyping kit from Isogen Lifescience (cat. no. IM9701). 182 Eur
J Clin Microbiol Infect Dis (2011) 30:181–191 Page 3. Spoligotypes in binary format were entered
in an Excel spreadsheet and compared with the spoligotyping database SpolDB4 [13]. …
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ROLE OF 2-MONTH SPUTUM SMEARS IN PREDICTING CULTURE CONVERSION IN PULMONARY TUBERCULOSIS
WJ Su, JY Feng, YC Chiu… – European Respiratory …, 2011 – Eur Respiratory Soc
… All clinical isolates were genotyped by commercial spoligotyping kit (Isogen Bioscience BV,
Maarssen, the Netherlands). The “Beijing strain” was defined as deletion from spacer 1 to spacer
34 in the direct repeat region and the presence of (at least three of) spacers 35–43. …
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T JAGIELSKI, EWAA KOPEĆ… – Polish journal of …, 2011 – pjm.microbiology.pl
… Spoligotyping was perfor- med with a commercially available kit (Isogen Bio- science BV … e isolates
whose spoligotype patterns were already recorded in the database, were assigned “shared types”,
whereas those of spoligotypes identified for the first time, were …
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OY Limanskaya… – Cytology and Genetics, 2011 – Springer
… The PCR was carried out by a dry kit of reagents for DNA amplification (Isogen, Russian
Federation) in 20–50 μl of reaction mixture containing 4 mM NaCl, 50 mM KCl, 12 mM Tris HCl
(pH 8.0), 2.5 mM MgCl2, 200 μM dNTPs, 0.1–0.5 μM of each primer, and 1 unit of Taq DNA …
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STREPTOMYCIN RESISTANCE AND LINEAGE-SPECIFIC POLYMORPHISMS IN MYCOBACTERIUM TUBERCULOSIS GIDB GENE
FS Spies, AW Ribeiro, DF Ramos… – Journal of clinical …, 2011 – Am Soc Microbiol
… Genotyping.Spoligotyping was performed using a commercial kit (Isogen Biosciences BV,
Netherlands … it was classified as LAM3 and S/convergent sublineage by spoligotyping but as non …
Spoligotypes evolve through successive loss of spacer DNA sequences (16), and since …
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YL Guo, Y Liu, SM Wang, CY Li… – … of Tuberculosis and …, 2011 – ingentaconnect.com
… Of the (Isogen Bioscience BV, De Meern, The Netherlands). … 1–1800 = shared spoligotypes. Table
3 Results of MIRU typing and comparison of the three typing methods Isolate MIRU locus number
IS6110-RFLP Cluster 2 4 10 16 20 23 24 26 27 31 39 40 Spoligotyping MIRU …
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SF Huang, WJ Su, HY Dou, JY Feng… – Infection, Genetics and …, 2011 – Elsevier
… Spoligotyping was performed according to the method reported by Kamerbeek et al. (Kamerbeek
et al., 1997) using a commercial kit (Isogen Biosolutions, Netherlands). The spoligotypes were
identified according to the database SpoDB4.0 (Brudey et al., 2006), and the SIT …
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THE GENOTYPE CHARACTERISTIC OF CLINICAL ISOLATED STRAINS GETTING FROM BONE TUBERCULOSIS
LI Wen-sheng, LIU Yi, SUN Yong… – Chinese Journal of …, 2011 – global.clinicmed.net
… TM 琼脂糖购自赛百盛基因技 术有限公司。 DNA Ladder Marker 购自 TaＫaRa 公司。
凝胶成像仪购自 FOTO DＹNE 公司。 Spoligotyping 试 剂盒以及 Miniblotter MN４５为荷兰
Isogen Biosｃienｃe 公司产品。 链霉亲和素为 Sigam 公司产品。 化学发光 …
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MULTIPLE STRAINS OF< I> MYCOBACTERIUM BOVIS</I> REVEALED BY MOLECULAR TYPING IN A HERD OF CATTLE
EES Figueiredo, DF Ramos, L Medeiros… – The Veterinary …, 2011 – Elsevier
… to the spoligo-membrane was performed according to the manufacturer’s instructions (Isogen
Life Sciences). … The strains clustered by spoligotyping were analysed by VNTR-PCR to confirm
clonal relationships … Four spoligotypes were identified among the 12 M. bovis isolates. …
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FS Spies – 2011 – lume.ufrgs.br
… restriction fragment lenght polymorphism) da sequência de inserção IS6110, spoligotyping e
unidade de repetição de micobactéria- repetição em tandem de número variável (MIRU- …
Spoligotyping é uma técnica de genotipagem que explora a variabilidade da região …
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Y Hu, X Ma, EA Graviss, W Wang… – Epidemiology and …, 2011 – Cambridge Univ Press
… Spoligotyping was performed by using a com- mercial kit from Isogen Bioscience BV (Maarssen …
0.04), and resistance to RIF and SM (P=0.04) (Table 1). Spoligotyping identified 243 … Thirty-three
(9.4%) isolates had unique/novel spoligotypes, of which similarity analysis found that …
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[HTML] MODERN AND ANCESTRAL GENOTYPES OF MYCOBACTERIUM TUBERCULOSIS FROM ANDHRA PRADESH, INDIA
SK Thomas, CC Iravatham, BH Moni, A Kumar… – PloS one, 2011 – dx.plos.org
… the help of commercially available line probe arrays (Isogen Biosciences BV … tuberculosis complex
genetic diversity: mining the fourth international spoligotyping database (SpolDB4 … KP (2006)
Identifying Mycobacterium tuberculosis complex strain families using spoligotypes. …
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GSS Eldin, I Fadl-Elmula, MS Ali… – BMC infectious …, 2011 – biomedcentral.com
… 21] using a commercial kit to test for 43 individual spacers (Isogen, IJsselstein, The … Spoligotyping
provided a relatively simple and rapid means of describing the population of … improved
discrimination of strains sharing the CAS1_Dehli and Beijing spoligotypes when using MIRU …
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TM Hlokwe, AO Jenkins, EM Streicher… – … Journal of Veterinary …, 2011 – scielo.org.za
… One of the observed spoligotypes (SB0130) was dominant and represented 75% of isolates …
Spoligotyping Spoligotyping was done according to a standardised international method
(Kamerbeek et al. 1997) using a commercially available kit (Isogen Biosciences BV, Maarsen). …
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GENOTYPING DID NOT EVIDENCE ANY CONTRIBUTION OF MYCOBACTERIUM BOVIS TO HUMAN TUBERCULOSIS IN BRAZIL
A Rocha, AR Elias, LF Sobral, DF Soares… – Tuberculosis, 2011 – Elsevier
… using PCR and hybridization conditions on a commercially available membrane (Isogen
Bioscience BV … All isolates that yielded spoligotypes that were recognized as not previously
described patterns were submitted to at least one additional spoligotyping assay and …
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CK Mlambo – 2011 – wiredspace.wits.ac.za
… 61 Table 3.6 Description of the orphan MIRU patterns (n=45) from this study and
corresponding spoligotyping-based information (octal code, SIT, and clade)…………
64 Table 3.7 MIRU profiles of the four newly created spoligotypes (10 …
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USE OF SPOLIGOTYPE ANALYSIS TO DETECT LABORATORY CROSS-CONTAMINATION
B Nivin, J Driscoll, T Glaser, P Bifani… – Infection control and …, 2000 – JSTOR
… The spoligotyping analysis was performed using a commercially available kit (Isogen Bioscience
BV … In several cases of identical spoligotypes, the Wadsworth Center indicated that the …
Spoligotyping is an effective, relatively inexpensive (approximately 10% the cost of RFLP …
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CLINICAL AND GENOTYPIC CHARACTERISTICS OF CHILDHOOD TUBERCULOSIS IN CHONGQING, CHINA
L Xing, R Liu, Q Li, Z Peng… – European Journal of Clinical …, 2011 – Springer
… Of 105 isolates spoligotyped, the major spoligotypes found were … Spoligotyping was performed
according to the internation- ally standardized protocol [5]. A commercially available spoligotyping
kit (Isogen Bioscience BV, Maarssen, The Netherlands) was used as described …
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TUBERCULOSIS DUE TO MYCOBACTERIUM BOVIS AND MYCOBACTERIUM CAPRAE IN SHEEP
M Muņoz Mendoza, L Juan, S Menéndez… – The Veterinary …, 2011 – Elsevier
… by hybridisation of the biotin-labelled PCR product onto spoligotyping membrane (Isogen
Bioscience BV). … Isolates were characterised by spoligotyping as M. caprae SB0157 (flock A) and
M. bovis … close contact with cattle or goats infected by the same spoligotypes (Table 1 …
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[HTML] MYCOBACTERIUM BOVIS INFECTION IN GOATS FROM THE NORTHEAST REGION OF BRAZIL
SSS Higino, SR Pinheiro, GO Souza… – Brazilian Journal of …, 2011 – SciELO Brasil
… Isolate was characterised by spoligotyping as described (7). The amplified product
was detected by hybridization of the biotin-labelled PCR product onto spoligotyping
membrane (Isogen Bioscience BV, Maarssen, The Netherlands). …
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JUNREN SUN, HYUN DOU, SYI LEE… – Apmis, 2011 – Wiley Online Library
… on isolates from areas where the Beijing strain is prevalent as most isolates in this family share
the same spoligotypes (1, 5 … Spoligotyping … to the manufacturers’ protocol, to a set of 43 oligonucle-
otides of DR spacers on a membrane using the Isogen Bioscience spoligotyping kit …
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2010
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L Flores, LG Jarlsberg, EY Kim… – Journal of clinical …, 2010 – Am Soc Microbiol
… 2004 was performed with standardized methods (15) using a membrane with 43 oligonucleotides
representing the different direct variable repeats (DVRs) immobilized in specific locations (Ocimum
Biosolutions, Inc., Gaithersburg, MD). Beginning in 2004, spoligotyping of the …
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A Von Groll, A Martin, C Felix… – FEMS Immunology …, 2010 – Wiley Online Library
… RD Rio BrgR: 5′-CACCGCCACGCTGAATGAGACCA-3′, –. Genotyping by
spoligotyping. Spoligotyping was performed with a commercial kit (Ocimum Biosolutions
BV, India) according to standard procedures (Kamerbeek et al., 1997). …
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[HTML] Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype
A Von Groll, A Martin, M Stehr, M Singh, F Portaels… – PloS one, 2010 – dx.plos.org
… Strains were fully characterized and analyzed for the presence of mutations conferring resistance
to RMP and INH. Spoligotyping was performed with a commercial kit (Ocimum Biosolutions BV,
India) according to standard procedures [29]. Molecular characterization. …
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SO Viegas, A Machado, R Groenheit… – BMC …, 2010 – biomedcentral.com
… using a commercially available kit (Isogen Life Science BV, Utrecht, The Netherlands).
Spoligotyping results were analysed with the BioNumerics Software ver. 5.01 (Applied Maths,
Kortrijk, Belgium). Database comparison and geographical distribution of spoligotypes. …
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[PDF] from academicjournals.org
J Zhang, X Wu, Y Yang, Y Zhuang… – African Journal of …, 2010 – academicjournals.org
… The amplified PCR products were hybridized with spoligotyping Biodyne C membrane immobilized
43 oligonucleotide probes (Isogen Life Science, Holland). … A total of 8 distinct spoligotyping patterns
were observed. … 7 isolates had unique spoligotypes (Figure 1, Tables 1 and 2 …
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PC Chuang, YMA Chen, HY Chen… – Infection, Genetics and …, 2010 – Elsevier
… 2.2. Genotype analysis. Spoligotyping using a commercially available kit (Isogen Bioscience
BV, Maarssen, The Netherlands … Spoligotypes and RFLP patterns were scanned and analyzed
with Bionumerics ® software (version 4.601; Applied Maths, Kortrijk, Belgium). …
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[HTML] ASSOCIATIONS BETWEEN MYCOBACTERIUM TUBERCULOSIS STRAINS AND PHENOTYPES
T Brown, V Nikolayevskyy, P Velji… – Emerging Infectious …, 2010 – ncbi.nlm.nih.gov
… Spoligotyping was performed according to the manufacturer’s instructions (Isogen Lifescience,
IJsselstein, the Netherlands) (9). Images were digitized and entered into BioNumerics software
by using the BNIMA module (Applied Maths). Spoligotypes were assigned to families …
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F Milián Suazo, B Harris, C Arriaga Díaz… – Revista mexicana de …, 2010 – scielo.org.mx
… Spoligotyping se realizó tal como lo describe Kamerbeek et al (17) . De manera
resumida, la amplificación del … por 30 seg. La hibridización se realizó de acuerdo
a las recomendaciones del fabricante (Isogen). El ADN hibridado …
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EN Noguti, CQF Leite, AC Malaspina… – Memórias do Instituto …, 2010 – SciELO Brasil
… Spoligotyping – Spoligotyping was performed in all 93 M. tuberculosis isolates, using the standard
method … of 43 spacer oligonucleotides covalently linked to the spoligo-membrane (Isogen Life
Sciences … Spoligotypes were reported using an octal code in which the 43-digit binary …
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Y Hu, S Hoffner, W Jiang… – BMC infectious …, 2010 – biomedcentral.com
… Spoligotyping was carried out by using the commercial kit from Isogen Bioscience BV … Table 3.
Spoligotyping pattern and frequency of the MTB isolates included. … B, Kurepina N, Bennett KP:
Identifying Mycobacterium tuberculosis complex strain families using spoligotypes. …
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[HTML] MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM BOVIS ISOLATES FROM ETHIOPIAN CATTLE
D Biffa, E Skjerve, J Oloya, A Bogale… – BMC veterinary …, 2010 – biomedcentral.com
… spacers were then hybridized with the known oligonucleotide spacers ligated to the membrane
(Isogen). … The discriminatory power (HGDI) of the spoligotyping method was calculated to be 0.81.
Also on the basis of the above findings (the number of distinct spoligotypes (S) = 12 …
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[HTML] MYCOBACTERIUM TUBERCULOSIS BEIJING STRAIN, BAMAKO, MALI
B Diarra, S Siddiqui, D Sogoba… – Emerging infectious …, 2010 – ncbi.nlm.nih.gov
… See the article”Global Distribution of Mycobacterium tuberculosis Spoligotypes” in volume 8 on
page 1347. … Spoligotyping using a commercially available kit (Spoligotyping Isogen Life Science,
De Meern, the Netherlands) showed characteristics of the Beijing clade (Appendix …
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EVALUATION OF 24 LOCUS MIRU-VNTR GENOTYPING OF MYCOBACTERIUM TUBERCULOSIS ISOLATES IN CANADA
S Christianson, J Wolfe, P Orr, J Karlowsky… – Tuberculosis, 2010 – Elsevier
… some cases. 22 Blots were purchased from Isogen Biosolutions and run according
to the manufacturer’s directions. Spoligotyping patterns were converted to the “octal”
pattern using the previously described formula. 23 Data …
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C McGoldrick, C Coghlin, A Seagar… – BMJ Case …, 2010 – casereports.bmj.com
… At SMRL spoligotyping was performed using a commercial assay available from Isogen Life
Science (3600 BT; Maarssen, The Netherlands) and 15-locus Mycobacterial Interspersed
Repetitive Unit-Variable Number Tandem Repeat (MIRU-VNTR) typing, performed using the …
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MOLECULAR EPIDEMIOLOGY OF HUMAN CASES OF TUBERCULOSIS BY MYCOBACTERIUM BOVIS IN MEXICO
F Milián-Suazo, L Pérez-Guerrero… – Preventive veterinary …, 2010 – Elsevier
… Coding allows easy comparisons of spoligotypes from different laboratories and from different
parts of … Spoligotyping was performed following a standard protocol (Kemerbeek et al., 1997). … 6
spacer sequences from M. bovis BCG were covalently bound (ISOGEN, Maarssen, the …
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MA Merza, P Farnia, AM Salih, MR Masjedi… – …, 2010 – content.karger.com
… [19] , with a commercially available kit, according to the manufacturer’s instructions (Isogen Biosci-
ence, The … Analysis of Spoligotyping Data A total of 133 distinct spoligopatterns were identi- fied …
The distribution of spoligotypes (T, CAS, EAI, Haarlem, X, Beijing, Or- phan and …
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[HTML] CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS BEIJING ISOLATES FROM THE MEDITERRANEAN AREA
M Alonso, NA Rodriguez, C Garzelli… – BMC …, 2010 – biomedcentral.com
… deletion of the spacers 1-34 and the characteristic hybridization pattern of spacers 35-43, and
the remaining seven corresponded to variant “Beijing-like” spoligotypes. … Spoligotyping was
performed following the manufacturer’s instructions (Isogen, Netherlands). …
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T Jagielski, E Augustynowicz-Kopeć… – Journal of clinical …, 2010 – Am Soc Microbiol
… CTAB) method (47) were stored at −20°C before being subjected to spoligotyping using a …
hybridized to a set of 43 oligonucleotides covalently bound to a membrane (Isogen Life Science
BV … Spoligotypes in binary format were converted to an octal code for comparison with the …
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R Lopez-Alvarez, C Badillo-Lopez… – BMC …, 2010 – biomedcentral.com
… To determine lineage, the 57 strains (48 MTb and 9 M. bovis) from the MTC were spoligotyped
and binary outcomes were compared with the shared type (ST) number and lineages and
sublineages reported by … Spoligotyping. … Spoligotypes were reported using an octal code [74]. …
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CC Lai, CK Tan, SH Lin, CH Liao… – Chest, 2010 – chestjournal.chestpubs.org
… Spoligotyping was performed according to the internationally standardized protocol to identify
and differentiate … 10 A commercially available kit (Isogen Bioscience BV; Maarssen, The
Netherlands) was … database was used to assign genotype families to the spoligotypes obtained …
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K Mallard, R McNerney, AC Crampin… – Journal of clinical …, 2010 – Am Soc Microbiol
… on these isolates with the aid of a commercially available kit (Isogen Biosciences BV … These isolates
were also spoligotyped and had previously been characterized by IS6110-RFLP … Spoligotyping
versus Beijing and LAM PCR results for 377 clinical M. tuberculosis isolates used …
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[HTML] CLUSTERING OF MYCOBACTERIUM TUBERCULOSIS CASES IN ACAPULCO: SPOLIGOTYPING AND RISK FACTORS
E Nava-Aguilera, E Harris, S Mitchell… – Clinical and …, 2010 – hindawi.com
… DNA with 43 oligonucleotides covalently linked to a nylon membrane (Isogen Bioscience BV …
SpolTools permitted the analysis of M. tuberculosis spoligotyping data to identify emerging … and
visualization of the probable evolutionary relationships between the spoligotypes in our …
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TC Rodwell, AJ Kapasi, M Moore… – International Journal of …, 2010 – Elsevier
… For this study, we also examined all available spoligotypes in the US national surveillance
database of M. bovis isolates sent to the National Veterinary Services Laboratories … Spoligotyping
was performed by all laboratories using the Spoligo Kit (Isogen Bioscience BV …
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S Rodríguez, B Romero, J Bezos, L De Juan… – Veterinary …, 2010 – Elsevier
… The isolates were spoligotyped following the protocol described by Kamerbeek et al … Frequencies
of the 43 spacers of the DR locus that are included in the spoligotyping membrane (designed …
Data expressed as percentage of spoligotypes (n = 252) in which the spacer is present …
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[HTML] from oxfordjournals.org
I Parwati, B Alisjahbana, L Apriani… – Journal of Infectious …, 2010 – jid.oxfordjournals.org
… of the Beijing genotype per se or to confounding by drug resistance, which has been linked to
the Beijing genotype in different settings [1]. We previously spoligotyped a large … Spoligotyping
was performed using a commercial kit (Isogen Bioscience) according to the …
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CA Molina-Torres, J Castro-Garza… – Journal of medical …, 2010 – Soc General Microbiol
… (1997) was used. PCR amplification of the direct repeat locus was performed on heat-treated
cell suspensions. Biotin-labelled PCR products were detected by hybridization onto a
spoligotyping membrane (Isogen Bioscience). Microarray analysis. …
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[HTML] DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS GENOTYPES CIRCULATING IN NDOLA, ZAMBIA
C Mulenga, I Shamputa, D Mwakazanga… – BMC infectious …, 2010 – biomedcentral.com
… Spoligotyping was performed using a commercial kit (Isogen Bioscience BV, Maarssen, The
Netherlands) according … Spoligotyping and MIRU-VNTR patterns were compared to the
international Spol DB4.0 … Spoligotypes that were not present in the Spol DB4.0 are referred to …
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PHENOTYPIC AND GENOTYPIC ANALYSIS OF MULTIDRUG-RESISTANT TUBERCULOSIS IN ETHIOPIA
M Agonafir, E Lemma… – … of Tuberculosis and …, 2010 – ingentaconnect.com
… to the extraction protocol described by van Soolingen et al.14 Spoligotyping was performed … the
commercially available kit from Isogen Bioscience BV, Maarssen, The Netherlands, according
to … Spoligotypes described only once (non-clustered) in this study and in the SpolDB4 …
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FM Suazoa, B Harrisb, CA Díazc… – Revista Mexicana de …, 2010 – scielo.org.mx
… Spoligotyping was performed essentially as described by Kamerbeek(17). Briefly,
amplification of DNA was run in … for 30 sec. Hybridization was performed as
recommended by the manufacturer (Isogen). Hybridized DNA was detected …
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CC Lai, CK Tan, SH Lin, CH Liao… – European journal of …, 2010 – Springer
… by serial dilution on agar plates as described previously [1]. Spoligotyping, according to … the M.
tuberculosis complex isolates [3]. A commercially available kit (Isogen Bioscience BV … 1
Dendrograms of spoligotypes generated by clustered Myco- bacterium tuberculosis strains after …
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OY Limanskaya, TN Mukhina, VN Stepanshina… – Molecular Biology, 2010 – Springer
… carrying AGC AGA; HB125, carrying AGC ACC, and H37Rv with the wild type codon
315 (negative control for amplification). The reaction mixture (20–50 μl) was prepared
from a dry amplification kit (Isogen, Russia). Its com …
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A Al-Maniri, JPN Singh, O Al-Rawas… – … of Tuberculosis and …, 2010 – ingentaconnect.com
… Figure Spoligotyped distribution of … Molecular typing of Mycobacterium tuberculosis isolates
from a rural area of Kanpur by spoligotyping and mycobacterial interspersed re- petitive units
(MIRUs) typing. … Predominant tubercu- losis spoligotypes, Delhi, India. …
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J Zhang, E Abadia, G Refregier… – Journal of medical …, 2010 – Soc General Microbiol
… Thus, as spoligotypes are considered both as phylogenetic and epidemiological markers (Asiimwe
et al., 2008; Sola et al., 2001; Tanaka & Francis, 2006 … Both sets had been previously genotyped
by spoligotyping using commercial membranes (Isogen Biosciences). …
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S Rosales, L Pineda-García… – BMC …, 2010 – biomedcentral.com
… All isolates were spoligotyped in order to identify the predominant genotypes within this … SG
performed and analyzed RFLP; carried out bioinformatics analysis of spoligotyping and RFLP
results … NR performed database analysis of the spoligotypes and helped draft the manuscript …
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JS Sutherland, BC de Jong, DJ Jeffries, IM Adetifa… – PloS one, 2010 – dx.plos.org
… 10ng of DNA was used for spoligotype analysis with commercially available membranes (Isogen
Biosciences, The Netherlands). … Contributed reagents/materials/analysis tools: BCdJ. Wrote the
paper: JSS MOCO. Performed spoligotyping: BCdJ. Statistical analysis: DJJ. …
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[BOOK] RAPID TESTS FOR MULTIDRUG RESISTANT TUBERCULOSIS IN LOW INCOME SETTINGS
F Bwanga – 2010 – mbl.mak.ac.ug
… In paper V, we applied spoligotyping to study the clustering rate and predominant genotypic strains
of 99 MDR-TB strains isolated from patients in Kampala. Eighty three percent of the strains
occurred in clusters, and the T2 lineage was the largest single cluster. Conclusion. …
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2009
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Spoligotyping for molecular epidemiology of the Mycobacterium tuberculosis complex
JR Driscoll – Methods Mol. Biol, 2009 – Springer
… 1. Spoligotyping membrane (Ocimum Biosolutions Inc., for- merly Isogen Biosciences BV … potential
of additional spacer sequences for “expanded” or “extended” spoligotyping (25, 26). … spacer
sequences would improve the differentiability of the commonly observed spoligotypes. …
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PJ Freidlin, D Goldblatt… – Journal of clinical …, 2009 – Am Soc Microbiol
… The fast DNA extraction method (amount used, 2 μl) was also successfully employed for
spoligotyping (Ocimum Biosolution Company, India). … In addition, further CAS MIRU-VNTR types
and associated spoligotypes were identified in the literature (3, 6, 7, 58, 61, 62). …
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MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS IN THE UNITED ARAB EMIRATES (UAE)
A Saaidi… – 2009 – qmro.qmul.ac.uk
… HYPERLINK \l “_Toc237239861” **3.2.11 Identification of the UAE isolates by spoligotyping *
PAGEREF _Toc237239861 \h **133**. * HYPERLINK \l “_Toc237239862” **3.2.12 Comparison
between MIRU-typed and spoligotyped families in association with katG463/gyrA95 …
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DD An, NTH Duyen, NTN Lan, DTM Ha… – Antimicrobial agents …, 2009 – Am Soc Microbiol
… with primers Dra (biotin labeled) and Drb, hybridized to the spoligotype membrane (Isogen
Bioscience BV … were assigned to the Euro-American clade on the basis of spoligotyping patterns …
LSP types and spoligotypes of 109 consecutive FQ-resistant isolates and 109 consecutive …
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[PDF] NOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSIS
TB Nosocomial – Indian J Tuberc, 2009 – lrsitbrd.nic.in
Page 1. Indian Journal of Tuberculosis [Indian J Tuberc 2009; 56:59-61] Editorial
NOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSIS
Indian Journal of Tuberculosis Vol. 56 New Delhi, April 2009 No. 2 …
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[HTML] SUPERVISED LEARNING FOR THE AUTOMATED TRANSCRIPTION OF SPACER CLASSIFICATION FROM SPOLIGOTYPE FILMS
D Jeffries, N Abernethy… – BMC bioinformatics, 2009 – biomedcentral.com
… a standardized method [4] using commercially prepared membranes from Isogen Biosciences
(Maarsen … Crawford JT: Transfer of a Mycobacterium tuberculosis genotyping method,
Spoligotyping, from a … AR, Tanaka MM: spolTools: online utilities for analyzing spoligotypes of …
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PJ Freidlin, D Goldblatt… – Journal of clinical …, 2009 – Am Soc Microbiol
… In addition, further CAS MIRU-VNTR types and associated spoligotypes were identified in …
Spoligotyping.Spacer oligonucleotide typing (spoligotyping) for 43 spacers of the direct repeat
region (9 … performed using a kit according to the manufacturer’s instructions (Isogen/Omicium …
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X Jiang, C Lu, F Gao, F Wang, W Zhang… – Microbes and …, 2009 – Elsevier
… Spoligotyping was performed as previously described [16 … in 2 × SSPE (1 × SSPE is 0.18 M NaCl,
10 mM NaH 2 PO 4 , and 1 mM EDTA) −0.5% sodium dodecyl sulfate (SDS) at 60 °C and then
incubated in 1:4000 diluted streptavidin peroxidase conjugate (Isogen Bioscience) for …
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JR Sun, SY Lee, HY Dou… – European Journal of Clinical …, 2009 – Springer
… Page 2. in the Isogen Bioscience spoligotyping kit (Isogen Life Science, the Netherlands). … Among
the 323 clinical MTB isolates, a total of 78 different spoligotypes were found. One hundred and
sixty-nine isolates were determined to belong to the Beijing family. …
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[HTML] Mycobacterium bovis en Argentina: aislamientos de gatos tipificados por spoligotyping
MJ Zumárraga, M Martínez Vivot… – Revista argentina de …, 2009 – SciELO Argentina
… (8) , and using the spoligotyping kit (Isogen Biosolutions BV … Each of the different spoligotypes
were allocated a number. … H37Rv (ATCC 27294) and M. bovis Bacillus Calmette-Guerin (BCG)
(ATCC 27289) were included as reference strains in each spoligotyping experiment. …
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Spoligotyping for molecular epidemiology of the Mycobacterium tuberculosis complex
JR Driscoll – Methods Mol. Biol, 2009 – Springer
… 1. Spoligotyping membrane (Ocimum Biosolutions Inc., for- merly Isogen Biosciences BV … potential
of additional spacer sequences for “expanded” or “extended” spoligotyping (25, 26). … spacer
sequences would improve the differentiability of the commonly observed spoligotypes. …
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BC De Jong, M Antonio, T Awine… – Journal of clinical …, 2009 – Am Soc Microbiol
… After spectrophotometry, we used 10 ng of DNA for spoligotype analysis (16) with commercially
available membranes (Isogen Biosciences, Maarssen, The Netherlands … If spoligotype analysis
did not result in a clear pattern of spacers, the isolate underwent repeat spoligotyping. …
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[HTML] from oxfordjournals.org
H Valvatne, H Syre, M Kross… – Journal of …, 2009 – Br Soc Antimicrob Chemo
… identification test (GenProbe Incorporated, San Diego, CA, USA) and by spoligotyping (Isogen
Bioscience BV … A total of 43 different spoligotypes were observed among the 96 isoniazid-resistant …
of the 63 isolates that could not be differentiated by spoligotyping produced diverse …
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S Tafaj, J Zhang, Y Hauck, C Pourcel… – Journal of clinical …, 2009 – Am Soc Microbiol
… ethambutol (2.0 μg/ml) (5). Identification at the species level was performed by phenotypic methods
and verified by a genotypic method on isolates with rare spoligotypes and/or … Spoligotyping was
performed using a commercial membrane (Isogen, The Netherlands …
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PICPI Cafrune, LGPLG Possuelo… – Canadian Journal …, 2009 – NRC Research Press
… was performed using a commercial kit according to the manufacturer’s instructions (Isogen
Biosciences BV … Table 1. Accuracy of spoligotyping results according to culture grading. … A total
of 44 different spoligotypes were identified; 112 isolates (83%) were included in 21 shared …
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MB Boniotti, M Goria, D Loda… – Journal of clinical …, 2009 – Am Soc Microbiol
… repeat locus were detected by hybridization onto a spoligotyping membrane (Isogen Bioscience
BV … into 64 different patterns, while the seven M. caprae spoligotypes were subdivided … The
discrimination provided by combination of spoligotyping and ETR methods allowed for a …
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HY Dou, JJ Lu, CW Lin, JR Chang, JR Sun… – Journal of microbiological …, 2009 – Elsevier
… Spoligotyping was carried out using a spoligotyping kit according to the manufacturer’s instructions
(Isogen Bioscience BV). Spoligotypes common to more than one strain were designated as shared
types (ST) and assigned a shared international type number (SIT) according to …
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[PDF] Comparison of phenotypic and genotypic methods for pyrazinamide susceptibility testing
S Shenai, C Rodrigues, M Sadani, N Sukhadia… – Indian J Tuberc, 2009 – medind.nic.in
… All the PZA resistant isolates were further spoligotyped to identify M. bovis, if present. … Finger
printing – Spoligotyping All the 63 culture isolates were subjected to spoligotyping using a
commercially available Isogen kit, Netherlands as described by Kamerbeek et al.11 Briefly …
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J Yorsangsukkamol, A Chaiprasert, T Prammananan… – Tuberculosis, 2009 – Elsevier
… 2.4. Spoligotyping. Spoligotyping was performed with a commercially available
kit (Isogen Bioscience BV, Maarsen, The Netherlands). … 25 All obtained spoligotypes
were compared to SpolDB4 for analysis. 2.5. Data analysis. …
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S Shakoor, M Tanveer, Y Rafiq… – Scandinavian …, 2009 – informahealthcare.com
… Five different resistance phenotypes were identified among the isolates spoligotyped: isolated
low-level isoniazid resistance (resistant to 0.2 µg/ml) in 1 isolate; high-level isoniazid resistance
(resistant to 1 … Spoligotypes Spoligotyping grouped 66 isolates into 9 different clusters. …
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BA van der Meer… – Factors Underlying the Success … – repository.ubn.kun.nl
… min. Spoligotyping was performed using a commercial kit (Isogen Bioscience,
Maarssen, The Netherlands). The … min. Spoligotyping was performed using a
commercial kit (Isogen Bioscience, Maarssen, The Netherlands). The …
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Emergence of new forms of totally drug-resistant tuberculosis bacilli
AA Velayati, MR Masjedi, P Farnia… – Chest, 2009 – chestjournal.chestpubs.org
… by polymerase chain reaction (PCR) using primers derived from the direct repeat sequence
(Isogen Bioscience; Maarssen, the … and CAS (n = 4; 17.5%) [Table 2]. The VNTR profiles of
spoligotypes strains were … Spoligotyping Patterns and VNTR Profiles of XDR and TDR Patients. …
Cited by 81 – Related articles – All 11 versions – Import into BibTeX
I Parwati, R van Crevel, J de Beer… – Factors Underlying the … – repository.ubn.kun.nl
… of rpoB-hotspot region Three hundred and twenty five spoligotyped isolates were … Mycobacterium
tuberculosis complex genetic diversity: mining the fourth international spoligotyping database
(SpolDB4 … mutations in katG, rpoB, rpsL and rrs genes with spoligotypes of multidrug …
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[HTML] from oxfordjournals.org
R Van Crevel, I Parwati… – Journal of Infectious …, 2009 – jid.oxfordjournals.org
… Mycobacterial DNA was extracted by bringing 2 loops of bacterial mass from a M.
tuberculosis culture in saline and subsequently heating it at 95°C for 5 min.
Spoligotyping was performed using a commercial kit (Isogen Bioscience). …
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ZH Helal, MSED Ashour, SA Eissa… – Journal of clinical …, 2009 – Am Soc Microbiol
… was amplified with primers DRa and DRb, and the amplified biotinylated products were hybridized
to a set of 43 oligonucleotides covalently bound to a membrane (Isogen Life Science BV …
Comparison of spoligotyping results with database types.Spoligotypes in binary …
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AA Velayati, P Farnia, TA Ibrahim… – …, 2009 – content.karger.com
… For spoligotyping, the direct repeat region was am- plified by PCR using primers derived from
the direct repeat se- quence (Isogen Bioscience). … Spoligopatterns and VNTR Profiles When the
spoligotype patterns were compared with earlier published spoligotypes [5] , our …
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Mycobacterium tuberculosis genotypic diversity in pyrazinamide-resistant isolates of Iran
F Doustdar, AD Khosravi… – Microbial Drug …, 2009 – online.liebertpub.com
… Spoligotyping was performed with a commercial kit (Isogen Bioscience BV, Maarsen, The … Only
1 (5.0%) of the 20 PZA-monoresistant isolates had spoligotyping patterns characteristic … Therefore,
the CAS superfamily spoligotypes showed no important correlation with MDR-TB as …
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Epidemiology of Mycobacterium bovis infection in wild boar (Sus scrofa) from Portugal
N Santos, M Correia-Neves… – Journal of wildlife …, 2009 – Wildlife Dis Assoc
… signatures were compared with those available in SpolDB4.0, the international spoligotyping
database (Brudey et al … The spoligotypes of 14 M. bovis isolates obtained from study areas B and
G … originated from study area B. Isolates from the study area C were not spoligotyped. …
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PI Cafrune – 2009 – lume.ufrgs.br
… Spoligotyping technique presented good results when applied directly to clinical samples, once
89% of samples presented agreeing results. Among 135 patients isolates genotyped, it was found
44 differnt spoligotypes, 112 (83%) were shared types and 23(17%) were unique. …
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BB Asiimwe, J Asiimwe, G Kallenius… – Veterinary …, 2009 – veterinaryrecord.bmj.com
… The isolates were spoligotyped using the DNA extracted at the identification stage. … The
spoligotypes were analysed by BioNumerics software, version 5.0 (Applied Maths) as character …
were compared with those in SpolDB4.0, the international spoligotyping database (Brudey …
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High resolution typing system for pathogenic Mycobacterium tuberculosum
PS Keim, RS Spurgiesz… – US Patent 7,592,135, 2009 – Google Patents
… Spoligotyping is currently the most widely used PCR- based method of M. tuberculosum
genotyping due to the 65 relative technical ease of … In a database of 3,319 spoligotypes from
isolates collected in geographically distinct areas, seven spoligotype patterns were found for …
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R Cicero, H Olivera, A Hernández-Solis… – European journal of …, 2009 – Springer
… a commercially available kit in accordance with the manufacturer’s recommendations (Isogen
Bioscience BV … and consistent identification of M. bovis was obtained by spoligotyping, showing
a … cattle, where even isolates from the same herd show different spoligotypes [26] and …
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R Stavrum, VP Myneedu, VK Arora, N Ahmed… – PloS one, 2009 – dx.plos.org
… C membranes with the 43 synthetic oligonucleotides covalently bound to the membranes (Isogen
Bioscience BV … (2004) Predominant tuberculosis spoligotypes, Delhi, India. … repetitive-
unit-variable-number tandem-repeat typing system combined with spoligotyping for population …
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Predominance of Mycobacterium tuberculosis EAI and Beijing lineages in Yangon, Myanmar
S Phyu, R Stavrum, T Lwin… – Journal of clinical …, 2009 – Am Soc Microbiol
… (35). Spoligotyping was performed with commercially available activated Biodyne C membranes
that had 43 synthetic oligonucleotides covalently bound to the membranes (Isogen Bioscience
BV … Prevalence of spoligotypes in Yangon.The spoligotyping results obtained …
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[HTML] Tokyo-172 BCG vaccination complications, Taiwan
R Jou, WL Huang… – Emerging Infectious Diseases, 2009 – ncbi.nlm.nih.gov
… BCG, DNA samples were initially screened using a GenoType kit (Hain Lifescience GmbH, Nehren,
Germany), multiplex PCR (7), and pncA sequencing (8). In addition, spoligotyping was performed
with a commercial kit (Isogen Bioscience BV, Maarssen, the Netherlands). …
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Immunologic analysis of HIV-uninfected taiwanese children with BCG-induced disease
WI Lee, FC Liang, JL Huang, TH Jaing… – Journal of clinical …, 2009 – Springer
… also used to identify and differentiate M. tuberculosis complex isolates in this study [38], using a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands). The resolved spoligotypes were M. tuberculosis complex strain specific. The spoligotyping profile of …
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X Jiang, C Lu, F Gao, F Wang, W Zhang… – Microbes and …, 2009 – Elsevier
… Spoligotyping was performed as previously described [16 … in 2 × SSPE (1 × SSPE is 0.18 M NaCl,
10 mM NaH 2 PO 4 , and 1 mM EDTA) −0.5% sodium dodecyl sulfate (SDS) at 60 °C and then
incubated in 1:4000 diluted streptavidin peroxidase conjugate (Isogen Bioscience) for …
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[PDF] NOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSIS
TB Nosocomial – Indian J Tuberc, 2009 – lrsitbrd.nic.in
Page 1. Indian Journal of Tuberculosis [Indian J Tuberc 2009; 56:59-61] Editorial
NOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSIS
Indian Journal of Tuberculosis Vol. 56 New Delhi, April 2009 No. 2 …
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DD An, NTH Duyen, NTN Lan, DTM Ha… – Antimicrobial agents …, 2009 – Am Soc Microbiol
… with primers Dra (biotin labeled) and Drb, hybridized to the spoligotype membrane (Isogen
Bioscience BV … were assigned to the Euro-American clade on the basis of spoligotyping patterns …
LSP types and spoligotypes of 109 consecutive FQ-resistant isolates and 109 consecutive …
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PJ Freidlin, D Goldblatt… – Journal of clinical …, 2009 – Am Soc Microbiol
… In addition, further CAS MIRU-VNTR types and associated spoligotypes were identified in …
Spoligotyping.Spacer oligonucleotide typing (spoligotyping) for 43 spacers of the direct repeat
region (9 … performed using a kit according to the manufacturer’s instructions (Isogen/Omicium …
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[HTML] Supervised learning for the automated transcription of spacer classification from spoligotype films
D Jeffries, N Abernethy… – BMC bioinformatics, 2009 – biomedcentral.com
… a standardized method [4] using commercially prepared membranes from Isogen Biosciences
(Maarsen … Crawford JT: Transfer of a Mycobacterium tuberculosis genotyping method,
Spoligotyping, from a … AR, Tanaka MM: spolTools: online utilities for analyzing spoligotypes of …
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Y Chen, Y Chao, Q Deng, T Liu, J Xiang, J Chen… – Tuberculosis, 2009 – Elsevier
… Spoligotyping was performed to define the genotype of Beijing family strains with a
commercial kit (Isogen Bioscience BV, Maarssen, The Netherlands) according to the
manufacturer’s instruction. The spoligotype that only contained …
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[PDF] Tuberculosis Control in Oman
A Al-Maniri – 2009 – diss.kib.ki.se
… Spoligotyping of 312 isolates of Mycobacterium tuberculosis from TB patients
diagnosed during 2005-2007 was carried out in study IV. … 25 6.5 Study IV: Biodiversity
and clustering of M.tuberculosis isolates using spoligotyping ….. …
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N Motamedi – 2009 – edoc.ub.uni-muenchen.de
… modernen und der ursprünglichen Form von M. tuberculosis. Van Embden et al. 68
untersuchten M. canetti mit einem erweiterten Spoligotyping (siehe unten), das mehr als
die 43 Spacer der etablierten Technik aufsucht. Hier fielen Spacer auf, …
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[HTML] Tuberculosis disparity between US-born blacks and whites, Houston, Texas, USA
JA Serpa, LD Teeter, JM Musser… – Emerging infectious …, 2009 – ncbi.nlm.nih.gov
… isolates underwent susceptibility and genotype testing (insertion sequence 6110 restriction
fragment length polymorphism, spoligotyping, and genetic … the spacer oligonucleotide type
(spoligotype) was determined by using a commercially available kit (Isogen Bioscience BV …
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Molecular characterization of Mycobacterium Bovis isolates from selected slaughter houses in Kampala
J Asiimwe – 2009 – dspace.mak.ac.ug
… Spoligotyping revealed that three of the M. bovis isolates had spoligopatterns that had previously
been reported in Uganda while the seven … Although a few clusters were identified on analysis
of the spoligotypes and RFLP patterns, no geographical sub-structuring was observed …
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Predictors of poor treatment outcome in multi-and extensively drug-resistant pulmonary TB
K Kliiman… – European Respiratory Journal, 2009 – ersj.org.uk
… The strains were spoligotyped as described by Kamerbeek et al. 17 using commercially available
membranes (Isogen, Maarssen, The Netherlands) 17. … Mycobacterium tuberculosis complex genetic
diversity: mining the fourth international spoligotyping database (SpolDB4) for …
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[HTML] Tuberculosis Disparity between US-born Blacks and Whites, Houston, Texas, USA
F Figures, I Capitalization, AS Guides… – Tuberculosis, 2009 – wwwnc.cdc.gov
… isolates underwent susceptibility and genotype testing (insertion sequence 6110 restriction
fragment length polymorphism, spoligotyping, and genetic … the spacer oligonucleotide type
(spoligotype) was determined by using a commercially available kit (Isogen Bioscience BV …
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Molecular epidemiology of Mycobacterium tuberculosis in the United Arab Emirates (UAE)
A Saaidi… – 2009 – qmro.qmul.ac.uk
… HYPERLINK \l “_Toc237239861” **3.2.11 Identification of the UAE isolates by spoligotyping *
PAGEREF _Toc237239861 \h **133**. * HYPERLINK \l “_Toc237239862” **3.2.12 Comparison
between MIRU-typed and spoligotyped families in association with katG463/gyrA95 …
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[PDF] EPIDEMIOLOGÍA MOLECULAR DE LA
TEN ALMERÍA – hera.ugr.es
Page 1. FACULTAD DE MEDICINA UNIVERSIDAD DE GRANADA DEPARTAMENTO
DE MEDICINA TÍTULO TESIS DOCTORAL: EPIDEMIOLOGÍA MOLECULAR DE LA
TUBERCULOSIS EN ALMERÍA Page 2. Editor: Editorial …
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2008
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PC Chuang, H Liu, C Sola, YMA Chen… – Infection, Genetics and …, 2008 – Elsevier
… A commercially available spoligotyping kit (Isogen Bioscience BV, Maarssen, The Netherlands)
was used as … All 138 isolates were successfully genotyped by spoligotyping, but only 122 were
of … A total of 27 spoligotypes were identified; 124 isolates (89.9%, 124/138) were …
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E Augustynowicz-Kopeć, T Jagielski… – Journal of clinical …, 2008 – Am Soc Microbiol
… Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen,
The Netherlands), according to the manufacturer’s instructions and as … All spoligotypes obtained
were compared to those in the world spoligotyping database (SpolDB4) at …
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B Asiimwe, S Ghebremichael… – BMC infectious …, 2008 – biomedcentral.com
… The obtained signatures were compared in binary format with the international spoligotyping
database of the Pasteur Institute of … Spoligotypes. To determine the strain lineages present in the
sample, the 344 isolates were spoligotyped and binary outcomes compared with those …
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P Sharma, DS Chauhan, P Upadhyay… – Infection, Genetics and …, 2008 – Elsevier
… 2.3. Spoligotyping. Spoligotyping to detect 43 spacers was done with the help of commercially
available kit (Isogen Biosciences, BV, Maarsen, The Netherlands). … 3.1. Spoligotyping. A total of
46 distinct spoligotypes were obtained from this set of 97 M. tuberculosis isolates. …
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JP Mathuria, P Sharma, P Prakash… – Infection, Genetics and …, 2008 – Elsevier
… spacers by using the standard method (Kamerbeek et al., 1997) with the help of commercially
available kit (Isogen Biosciences, BV … Spoligotyping. … Presence of 19 orphan isolates indicates
the need of conducting multicentric studies to know all the prevalent spoligotypes in Indian …
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F Al-Otaibi… – pjms.com.pk
… Spoligotyping was performed with a commer- cially available kit (Isogen Bioscience).6,7 RESULTS …
Culturing of phosphate buffer in MGIT tube was positive for MTB after seven-day inoculation. Ten
different spoligotypes were identified among the 47 MTB isolates. …
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B Romero, A Aranaz, Á Sandoval, J Álvarez… – Veterinary …, 2008 – Elsevier
… detected by hybridization of the biotin-labelled PCR product onto spoligotyping membrane (Isogen
Bioscience BV … The discriminatory capacity of spoligotyping was h = 0.38 … The spoligotypes SB0950,
SB1230 and SB1232 were shared between domestic and wildlife animals (red …
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Spoligotyping of Mycobacterium tuberculosis in anthracotic bronchitis
K Najafizadeh, F Ghorbani, P Farnia… – … of Tuberculosis and …, 2008 – ingentaconnect.com
… use at −20°C. Spoligotyping Spoligotyping was performed, as previously described
by Kamerbeek et al.,10 with a commercially available kit (Isogen Bioscience BV,
Maarssen, The Netherlands). Analysis Information about the …
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Molecular analysis of drug‐resistant Mycobacterium tuberculosis isolates collected in central Poland
E Augustynowicz‐Kopeć, T Jagielski… – Clinical …, 2008 – Wiley Online Library
… DNA was prepared by the cetyl-trimethyl-ammonium bromide method [4]. Spoligotyping was
performed using a commercially available kit (Isogen Bioscience BV … was also performed as
described previously [2]. Isolates with indistinguishable spoligotypes and IS6110 …
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[HTML] Human tuberculosis caused by Mycobacterium bovis, Taiwan
R Jou, WL Huang… – Emerging infectious diseases, 2008 – ncbi.nlm.nih.gov
… Currently, 119 M. bovis spoligotypes are contained in the fourth international spoligotyping
database (SpolDB 4) and … strains, presence or absence of region of difference 1 was analyzed
(5). Spoligotyping was performed with a commercial kit (Isogen Bioscience BV …
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M Alonso, S Borrell, MM Lirola, E Bouza… – Tuberculosis, 2008 – Elsevier
… Spoligotyping was performed using primers of the spoligotyping kit (Isogen, Netherlands) and
following the manufacturer’s instructions. The spoligotypes were analyzed in the MIRU-VNTRplus
application (www.miru-vntrplus.org) 19 in order to identify the genetic lineages …
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Characterization of extensively drug-resistant Mycobacterium tuberculosis clinical isolates in China
Z Sun, Y Chao, X Zhang, J Zhang… – Journal of clinical …, 2008 – Am Soc Microbiol
… repeat (MIRU-VNTR) analysis. Spoligotyping was performed with a commercially
available kit (Isogen Bioscience BV, Maarssen, The Netherlands) according to the
manufacturer’s protocols. PCR-based MIRU-VNTR analysis …
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PC Hill, DJ Jackson-Sillah, A Fox, RH Brookes… – PLoS One, 2008 – dx.plos.org
… For molecular subtyping of index case isolates, we extracted mycobacterial DNA using CTAB
and chloroform[18]. We performed spoligotyping using membranes from Isogen Biosciences
(http:/www.isogen-lifescience.com), as previously described[19]. …
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[PDF] : Transmission from South American Sea Lion () to Bactrian Camel () and Malayan Tapirs ()
I Moser, WM Prodinger, H Hotzel… – Veterinary …, 2008 – peer.ccsd.cnrs.fr
… by 30 cycles of 96°C for 15 sec, 67°C for 60 sec, 72°C for 60 sec, and a final extension at 18
72°C 300 sec. 19 Spoligotyping. Spoligotyping (9; Isogen Bioscience, The Netherlands) was
performed using 20 DNA extracted from cultures. 21 MIRU typing. …
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M Ashworth, KL Horan, R Freeman… – Journal of clinical …, 2008 – Am Soc Microbiol
… Only one isolate per case was included in the analysis. Genotyping.Spoligotyping
was performed according to previously published methods by using a spoligotyping
kit (Isogen, IJsselstein, The Netherlands) (12). MIRU analysis …
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J Oloya, J Opuda-Asibo, R Kazwala… – Epidemiology and …, 2008 – Cambridge Univ Press
… MTC were submitted for further differentiation by the spoligotyping kit ac- cording to the
manufacturer’s instructions (Isogen, Life Science … One of the spoligotypes (Hu-4 and Hu-5 in Fig.
1) is labelled LAM 3/S in the spoligotyping database SpolDB4, and has been isolated in 118 …
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Genotypic diversity of extensively drug-resistant tuberculosis (XDR-TB) in South Africa
CK Mlambo, RM Warren, X Poswa… – … of tuberculosis and …, 2008 – ingentaconnect.com
… Spoligotyping was performed ac- cording to the internationally standardised protocol11 using
commercially … of the genotyping data, serial isolates from 13 patients were randomly sampled
and spoligotyped. … was used to assign geno- type families to the spoligotypes obtained.12 …
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[HTML] Molecular epidemiology and evolutionary genetics of Mycobacterium tuberculosis in Taipei
HY Dou, FC Tseng, CW Lin, JR Chang… – BMC infectious …, 2008 – biomedcentral.com
… Of 356 isolates spoligotyped, 290 (81.4%) displayed known spoligotypes and 66 were not …
Spoligotyping was carried out according to the manufacturer’s instructions (Isogen Bioscience
BV … The resulting spoligotypes were documented under a binary code representing either a …
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[HTML] Genotyping and drug resistance patterns of M. tuberculosis strains in Pakistan
M Tanveer, Z Hasan, A Siddiqui, A Ali… – BMC Infectious …, 2008 – biomedcentral.com
… Spoligotyping of M. tuberculosis isolates. All M. tuberculosis strains were spoligotyped and their
data analyzed using UPGMA calculation in the Bionumerics software. … one (78%) isolates grouped
into 59 different “shared types” while 205 (22%) isolates had ‘orphan’ spoligotypes. …
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I Moser, WM Prodinger, H Hotzel, R Greenwald… – Veterinary …, 2008 – Elsevier
… 2.5. Spoligotyping. Spoligotyping (Kamerbeek et al., 1997; Isogen Bioscience, The Netherlands)
was performed using DNA extracted from cultures. 2.6. MIRU typing. MIRU typing was
performed targeting 12 chromosomal loci as given by Mazars et al. …
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Mycobacterium tuberculosis population structures differ significantly on two Indonesian Islands
I Parwati, R Van Crevel, M Sudiro… – Journal of clinical …, 2008 – Am Soc Microbiol
… Therefore, we have spoligotyped M. tuberculosis isolates from a large cohort of patients …
Mycobacterium tuberculosis complex genetic diversity: mining the fourth international
spoligotyping database (SpolDB4) for … Global distribution of Mycobacterium tuberculosis …
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Development of multiplex assay for rapid characterization of Mycobacterium tuberculosis
IL Bergval, R Vijzelaar, ER Dalla Costa… – Journal of clinical …, 2008 – Am Soc Microbiol
… Strains 1 to 29 were selected for their specific spoligotypes and were used to determine the
correlation between MLPA, the spoligotype, and the sequence of ogt codon 15 (a marker for the
M … Spoligotyping.Mycobacterium tuberculosis strains were spoligotyped by using a …
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Mycobacterium bovis in wildlife in France
G Zanella, B Durand, J Hars… – Journal of wildlife …, 2008 – Wildlife Dis Assoc
… Biotin-labeled PCR products were detected on a spoligotyping membrane (Isogen Bioscience BV,
Maarssen, The Netherlands). Purified sterile water and M. bovis and M. tuberculosis controls
included in the spoligomembrane kit were included in each batch of tests. …
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T Prammananan, W Cheunoy… – Clinical …, 2008 – Wiley Online Library
… In contrast, of 112 rifampicin-susceptible isolates that were spoligotyped, only 51 (45.5%) were
revealed to be … a One additional MDRTB isolate was not available for spoligotyping. … Association
of specific mutations in katG, rpoB, rpsL and rrs genes with spoligotypes of multidrug …
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US Rajapaksa, TC Victor, AJ Perera… – Transactions of the …, 2008 – Elsevier
… Spoligotyping was performed using a commercially available kit (Isogen Bioscience BV, Maarssen,
The … Spoligotyping of 98 M. tuberculosis isolates revealed a total of 43 patterns, with six … The finding
of identical spoligotypes in geographically widely separated countries may be …
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F Doustdar, AD Khosravi, P Farnia, AR Bahrmand… – Tanaffos, 2008 – nritld.ac.ir
… tuberculosis isolates are defined on the basis of shared spoligotypes or closely related IS6110 …
Spoligotyping was performed with a commercially available kit to detect 43 known spacers … DR
locus according to the instructions supplied by the manufacturer (Isogen Bioscience BV …
Cited by 9 – Related articles – View as HTML – All 6 versions – Import into BibTeX
Molecular epidemiology of Mycobacterium bovis: usefulness in international trade
F Milian-Suazo, B Harris, CA Díaz… – Preventive veterinary …, 2008 – Elsevier
… Spoligotyping was performed following the protocol described by Kamerbeek et al … Eighty-four
M. bovis strains from Mexico and the US were spoligotyped under the same protocol in two … When
comparing spoligotypes from the two laboratories, 50 (59%) out of the 84 strains were …
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Attempts to revive Mycobacterium tuberculosis from 300‐year‐old human mummies
E Lemma, O Zimhony, CL Greenblatt… – FEMS microbiology …, 2008 – Wiley Online Library
… Spoligotyping was performed at day 250 following the instructions of the manufacturer
of the ISOGEN Life Science Spoligotyping kit. Animal inoculations. Two sets of six
female guinea pigs weighing an average of 250 g were used. …
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Mycobacterium tuberculosis Uganda genotype is the predominant cause of TB in Kampala, Uganda
BB Asiimwe, T Koivula, G Kallenius… – … of Tuberculosis and …, 2008 – ingentaconnect.com
… Data analysis Spoligotypes were analysed using BioNumerics soft- ware (version 4.6, Applied
Maths, Kortrijk, Belgium) as a character type. … Spoligotyping To determine the predominant strain
lineages, the 344 isolates were spoligotyped and binary outcomes compared with …
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Pyrazinamide resistance among South African multidrug-resistant Mycobacterium tuberculosis isolates
M Mphahlele, H Syre, H Valvatne… – Journal of clinical …, 2008 – Am Soc Microbiol
… (i) Spoligotyping.Spoligotyping was performed to further … The resulting amplification products were
then hybridized to a commercially available membrane (Isogen Bioscience BV, Bilthoven, The
Netherlands), with covalently linked parallel rows of 43 synthetic oligonucleotides …
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LR Martinez, B Harris, WC Black… – Journal of Veterinary …, 2008 – vdi.sagepub.com
… The 6 isolates that comprised MLVA type N were divided into 3 spoligotypes: 1 isolate into SP2 …
A larger database of MLVA typed isolates, in combination with spoligotyping and conventional
epidemiological investigations, can provide a … n. Spacer sequences, Isogen Life Science …
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[CITATION] First Insight Into Mycobacterium tuberculosis Biodiversity in Sri Lanka: Evidence for Predominance of Beijing Genotype and Ancestral Lineages Among …
US Rajapaksa, AJ Perera, TC Victor… – International Journal of …, 2008 – Elsevier
[BOOK] Molecular characterization of Mycobacterium tuberculosis complex from Kampala, Uganda
B Asiimwe – 2008 – dspace.mak.ac.ug
… and colleagues (69), which is commercially available as a kit (Isogen Bioscience BV … the important
lineages and sub-lineages in SpolDB4.0, the international spoligotyping database iii … To investigate
the association between the predominant spoligotypes, HIV sero- status and anti …
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[PDF] A tuberkulózis molekuláris epidemiológiai vonatkozásai Magyarországon
L Judit – phd.sote.hu
Page 1. A tuberkulózis molekuláris epidemiológiai vonatkozásai Magyarországon
Doktori értekezés Lukács Judit Semmelweis Egyetem Klinikai Orvostudományok
Doktori Iskola Témavezetı: Dr. Somoskövi Ákos, MTA doktora …
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Y Casart, L Turcios, I Florez, R Jaspe, E Guerrero… – Tuberculosis, 2008 – Elsevier
… DR locus was carried out as described earlier, 15 with membranes supplied by Isogen Bioscience
BV … to mention that all the clones isolated from 1C8 or 1D3 displayed identical spoligotyping and
MIRU … A1 insertion 12 (Figure 1B), (ii) The IS6110-RFLPs and spoligotypes did not …
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Intérêt du typage moléculaire pour l’épidémiologie de la tuberculose en Limousin (1998–2006)
H Bezanahary, MC Baclet, C Sola… – Médecine et Maladies …, 2008 – Elsevier
… Keywords: MIRU15 typing; Mycobacterium tuberculosis; Spoligotyping. … L’hybridation (1H à 60
°C) des amplicons a été réalisée sur une membrane Isogen ® (Bioscience BV … sous forme d’un
code octal et comparés aux données de la banque de spoligotypes SpolDB4 (Institut …
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[PDF] TABLA 8.1 _ GEL DE CORRIMIENTO
TNN – Identificación y diagnóstico de Actinomicetales … – saber.ula.ve
… caontb. rivm. nl/Manuals/principl. html. Por su parte, Isogen Biosciences (http://www. isogen.
nl) ofrece membranas con ADN inmobilizado de espaciadores, para utilizarse en pruebas
de spoligotyping específicas para cepas de M. bovis/M. tuberculosis. …
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CLS Mucavele – 2008 – repository.utl.pt
… number of spoligotypes were identical to profiles found in the region. The data reported showed
that migration and HIV might be factors of dissemination of TB in Humans in Mozambique. Factors
that can compromise the performance of Spoligotyping technique and other …
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[BOOK] Molecular Characterisation of Antibiotic Resistance in Mycobacterium Tuberculosis
P Juréen – 2008 – diss.kib.ki.se
… personnel (91). In order to overcome this, spoligotyping was evolved. This … repeat) (91).
MIRU-VNTR analyses copy-numbers of repeats, however, in contrast to spoligotyping,
these have several sites scattered around the genome. Since …
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2007
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J Chen, AG Tsolaki, X Shen, X Jiang, J Mei… – Tuberculosis, 2007 – Elsevier
… Spoligotyping was performed by using an Isogen Bioscience spoligotyping kit (Isogen Life Science …
Figure 3. Beijing/W Spoligotypes observed in 182 isolates with deletion RD105. Comparison
of the multiplex PCR with spoligotyping revealed 100% sensitivity and specificity for …
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S David, DR Ribeiro, A Antunes, C Portugal… – Infection, Genetics and …, 2007 – Elsevier
… Spoligotyping was carried out according to the manufacturer’s instructions (Isogen Bioscience
BV, Maarsen, Netherlands). The resulting spoligotypes were documented under a binary code
representing either a positive or negative hybridization result (n and o) and analyzed …
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N Suresh, J Arora, H Pant, T Rana… – Journal of microbiological …, 2007 – Elsevier
… available kit, according to the instructions supplied by the manufacturer (Isogen Bioscience, BV …
a positive signal in spoligotyping, thus demonstrating the efficiency of spoligotyping in a … analysis
of DNA extracted from stored ZN slides identified tuberculosis spoligotypes in 41 …
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MT Javed, A Aranaz, L De Juan, J Bezos… – Tuberculosis, 2007 – Elsevier
… using two membranes: a commercial membrane containing the 43 spacers described earlier
23 (Isogen Bioscience BV … Number of spoligotyping patterns of M. bovis and M. caprae.
Thirty-one different spoligotypes were identified among the 308 M. bovis (26 patterns) and 88 …
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H Han, F Wang, Y Xiao, Y Ren… – Journal of medical …, 2007 – Soc General Microbiol
… To test the reproducibility of MIRU typing, we blindly typed 20 isolates in two independent
experiments. Spoligotyping. Spoligotyping was performed with a commercial spoligotyping kit
(Isogen Bioscience), according to the instructions supplied by the manufacturer. …
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K Kremer, BKY Au, PCW Yip, R Skuce, P Supply… – dare.uva.nl
… with a commercial membranee containing the standard set of 43 spacer oligonucleotides (Isogen
Bio- sciencee BV … The usee of spacers 56, 57, 66, 67, and 68 in spoligotyping increased the … patterns
among the Beijing lineage strains in this study from three to five spoligotypes.. …
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PJ Chin, CC Chiu… – Journal of clinical microbiology, 2007 – Am Soc Microbiol
… These isolates were characterized by IS6110 RFLP following standard protocols (12) and
spoligotyping with a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands)
(6). The characteristic … All other spoligotypes are defined as non-Beijing genotypes. …
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Znaczenie metody spoligotyping w epidemiologicznych dochodzeniach gruźlicy
E Augustynowicz-Kopeć… – Pneumonologia i …, 2007 – pneumonologia.viamedica.pl
… Autorzy dziękują Pani J. Scheenhart (Isogen Bioscience BV) za konsultację warunków repro …
IS6110 restriction fragment length polymor- phism patterns and spoligotypes determined by …
Spoligotyping of Myco- bacterium tuberculosis isolates from patients with pulmo- nary …
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C Rodrigues, D Almeida, S Shenai, N Goyal… – Indian Journal of …, 2007 – Medknow
… Spoligotyping Spoligotyping was performed with a commercially available kit (Isogen Bioscience). …
Remaining two isolates grown from smear positive specimens had different spoligotypes hence
were … Since spoligotyping is known to be less refractory for differentiation of M …
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C Rodrigues, D Almeida, S Shenai… – Indian Journal of …, 2007 – bioline.org.br
… Spoligotyping was performed with a commercially available kit (Isogen Bioscience). … Remaining
two isolates grown from smear positive specimens had different spoligotypes hence were … Since
spoligotyping is known to be less refractory for differentiation of M. tuberculosis, we …
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[HTML] Characterisation of mycobacteria isolated from slaughter cattle in pastoral regions of Uganda
J Oloya, R Kazwala, A Lund… – BMC …, 2007 – biomedcentral.com
… in mid-central and mid-western areas of Uganda and detected new and specific spoligotypes
not reported … Cultures belonging to the M. tuberculosis complex were submitted for further
differentiation by Spoligotyping [20] as described by the producer (Isogen, Life science …
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O Kisa, A Albay, O Baylan, E Tozkoparan… – Research in …, 2007 – Elsevier
… Spoligotyping was performed using a commercial kit (Isogen Bioscience BV, Maarssen, The
Netherlands) according to the … 2.6. Comparison of spoligotypes with the international spoligotype
database. Spoligotyping results were entered in a binary format and octal code on Excel …
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[HTML] from oxfordjournals.org
M Pillay… – Clinical infectious diseases, 2007 – cid.oxfordjournals.org
… Spoligotyping. Spoligotyping was performed using a kit from Isogen-Life Science
in accordance with the manufacturer’s instructions. The presence or absence of 43
spacers in the direct-repeat region of isolates of M. tuberculosis …
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[HTML] Longitudinal assessment of an ELISPOT test for Mycobacterium tuberculosis infection
PC Hill, RH Brookes, A Fox, D Jackson-Sillah… – PLoS medicine, 2007 – dx.plos.org
… We performed spoligotyping using membranes from Isogen Biosciences (http://www.isogen–
lifescience.com), as previously described [18] and analysed the results with software that we
designed using Matlab software (MathWorks, http://www.mathworks.com). …
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GS Kibiki, B Mulder, WMV Dolmans… – BMC …, 2007 – biomedcentral.com
… with samples which were INH-resistant, contained phenotypic rifampicin-resistant strains, or had
selected spoligotypes; phenotypic rifampicin … Spoligotyping. … 1 U/μl). Amplification and hybridization
were performed according to the manufacturer’s manual (Isogen Bioscience BV …
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Outbreak of Mycobacterium bovis infection in a wild animal park
SM Schmidbauer, P Wohlsein, G Kirpal… – Veterinary …, 2007 – veterinaryrecord.bmj.com
… and subtype of the MTC agent, 17 isolates from various organs were spoligotyped (Kamerbeek
and … FIG 2: Spoligotyping pattern of 17 mycobacterial isolates from various organs from seven …
in wild animals in Germany and in these cases the spoligotypes normally correspond to …
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Molecular investigation of recurrent tuberculosis in patients from Rwanda
AN Umubyeyi, IC Shamputa… – … of Tuberculosis and …, 2007 – ingentaconnect.com
… DNA fingerprinting techniques Spoligotyping was performed using a commercial kit (Isogen
Bioscience BV … 4 of the 12 previously cured MDR-TB patients showed different spoligotypes,
suggesting re … of their recurrence (Figures 1 and 2). In contrast, the spoligotyping pat- terns of …
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[HTML] Using ELISPOT to expose false positive skin test conversion in tuberculosis contacts
PC Hill, DJ Jeffries, RH Brookes, A Fox… – PLoS One, 2007 – dx.plos.org
… spectrophotometry. We performed Spoligotyping using membranes (Isogen
Biosciences), as previously described,[14] scanned the results and analysed them
with software designed in Matlab. Data management and analysis. …
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[PDF] Usefulness of molecular techniques to identify ongoing tuberculosis transmission in Saudi Arabia
SA Al-Hajoj, VK Mohammed… – Saudi medical journal, 2007 – smj.org.sa
… The labeled PCR product was used as a probe to hybridize with 43 synthetic spacer
oligonucleotides attached to a carrier membrane (Isogen Bioscience BV, Maarssen, The
Netherlands). The 2 isolates were identical by both RFLP and spoligotyping methods Figure …
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C Ködmön, S Niemann, MC Gutierrez… – Infection, Genetics and …, 2007 – Elsevier
… Spoligotyping was performed with a commercially available kit (Isogen Bioscience
BV, Maarssen, The Netherlands) according to the instructions of the manufacturer
(Kremer et al., 1999). Briefly, genomic mycobacterial DNA …
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LCO Lazzarini, RC Huard, NL Boechat… – Journal of clinical …, 2007 – Am Soc Microbiol
… Spoligotyping.All M. tuberculosis strains were spoligotyped using a commercial kit (Isogen
Bioscience BV … as an octal code (9). The person who performed the spoligotyping was blinded
to … information on the shared-type distributions of M. tuberculosis spoligotypes worldwide (N …
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YJ Sun, ASG Lee, SY Wong… – … of Tuberculosis and …, 2007 – ingentaconnect.com
… Spoligotyping Spoligotyping was performed using a commercial kit obtained from Isogen
Bioscience BV … as previously described.21,22 IS6110 RFLP patterns and/or MIRU-VNTR patterns
were used to determine genotypes for a few isolates with indeterminate spoligotypes. …
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Evidence of ‘amplifier effect’in pulmonary multidrug-resistant tuberculosis: report of three cases
A Umubyeyi, IC Shamputa, L Rigouts… – International Journal of …, 2007 – Elsevier
… Spoligotyping. Spoligotyping was performed with a commercial kit (Isogen Bioscience
BV, Maarssen, The Netherlands) by the previously described method. 10 Briefly, 4
μl of the heat-killed bacterial suspension from each sample …
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P Escalante – 2007 – digitallibrary.usc.edu
… BioImage Whole Band Analyzer™, version 3.2 software (BioImage, Ann Arbor, MI). Spoligotyping
was performed using the Isogen™ kit (Isogen, Bioscience, BV Maarseen, Netherlands) for all
isolates according to the manufacturer instructions. In addition, all …
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AC PANUNTO – crt.saude.sp.gov.br
… 35 Tabela 3- Perfis agrupados de IS6110-RFLP presentes no estudo….. 50
Tabela 4- Perfis agrupados de “Spoligotyping” presentes no estudo….. 53
Tabela 5- “Spoligotypes” mais freqüentes no estudo….. 54 …
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P Escalante – 2007 – books.google.com
… software (BioImage, Ann Arbor, MI). Spoligotyping was performed using the Isogen™
kit (Isogen, Bioscience, BV Maarseen, Netherlands) for all isolates according to
the manufacturer instructions. In addition, all isolates also …
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First insight into the population structure of Mycobacterium tuberculosis in Saudi Arabia
SAM Al-Hajoj, T Zozio, F Al-Rabiah… – Journal of clinical …, 2007 – Am Soc Microbiol
… to inter-DR spacer oligonucleotides covalently bound to a membrane (Isogen Life Science … All
spoligotypes were converted into octal format within Excel spreadsheets (8) and … The individual
spoligotyping patterns were compared with an updated SpolDB4 database named …
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Transmission of< i> Mycobacterium tuberculosis</i> in a Family Proved by Genotyping
CY Yang, R Jou, PC Chuang, JT Chang… – Journal of the Formosan …, 2007 – Elsevier
… Spoligotyping Spoligotyping is a polymerase chain reaction (PCR)-based analysis that uses
a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands). … The re-
solved spoligotypes were M. tuberculosis complex strain-specific. …
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[PDF] Molecular characterization of Mycobacterium tuberculosis isolates from Izmir, Turkey
C Bicmen, N Esen, EA Graviss… – … -Quarterly Journal of …, 2007 – microbiologica.net
… were analyzed with the BioImage (Ann Arbor, Mich.,USA) Whole Band Analyzer, version 3.4.2.
Spoligotyping was per- formed with a commercially available kit (Isogen Bioscience BV, Maarssen,
The Netherlands) in accordance with the manufacturer’s recom- mendations. …
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Instability of IS6110 patterns in multidrug-resistant strains of Mycobacterium tuberculosis
P Farnia, MR Masjedi, B Nasiri… – Epidemiology and …, 2007 – Cambridge Univ Press
… [12] with a commercially avail- able kit (Isogen Bioscience BV … Stability of M. tuberculosis IS6110
restriction fragment length polymorphism patterns and spoligotypes determined by … expanding
clones of M. tuberculosis and their global distribution assessed by spoligotyping in an …
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X Jiang, Y Zhang, W Zhang, F Gao, J Jin… – Microbes and …, 2007 – Elsevier
… SDS) at 60 °C and then incubated in 1:4000 diluted streptavidin–peroxidase conjugate (Isogen
Bioscience) for … This finding, which showed a spoligotyping pattern with hybridization only to the
spacer 35 to 43 … 1. Spoligotypes of the 56 M. tuberculosis clinical isolates from China. …
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GS Kibiki, B Mulder… – Tropical Medicine & …, 2007 – Wiley Online Library
… Spoligotyping patterns were analysed with Bionumerics 4.0 (Applied Maths, St-Martin-Latem,
Belgium). … strains in 25 of the 28 BAL samples with positive MTB culture could be spoligotyped.
In all 25, the spoligotypes indicated that the isolates belonged to M. tuberculosis complex …
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[PDF] MYCOBACTERIOSIS IN WILD BOAR: RESULTS OF 2000-2006 ACTIVITY IN NORTH-WESTERN ITALY
A Dondo, S Zoppi, F Rossi, L Chiavacci, A Barbaro… – aeema.vet-alfort.fr
… 37 a PCR based reverse line blot method covalently (Isogen, Netherland). M. bovis strains isolated
were further characterized by VNTR typing (ETR A, B, C, D, E) according to Frothingham
[Frothingham et al., 1998]. The strains unidentified by Spoligotyping were processed by a …
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VV Nikolayevskyy, TJ Brown… – Clinical …, 2007 – Wiley Online Library
… [23] using commercially available membranes (Isogen, Maarssen, The … binding to the
corresponding targets, but an association between these specific spoligotypes and mutations
in … Spoligotyping results demonstrated a relative diversity in the genetic structure of M. tuberculosis …
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J Dormandy, A Somoskovi, BN Kreiswirth… – Chest, 2007 – chestjournal.chestpubs.org
… Spoligotyping, a polymerase chain reaction-based genotyping method that detects 43 known
spacer sequences that are interspersed between the direct repeats in the genomic repeat region
of M tuberculosis, was performed with a commercially available kit (Isogen Bioscience …
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Új Mycobacterium tuberculosis törzs okozta mikroepidémia budapesti hajléktalanokban
C Ködmön, J Lukács, S Dávid, I Vadász… – Orvosi Hetilap, 2007 – akademiai.com
… A spoligotyping kevésbé érzékeny, mint az IS6110-RFLP fin- gerprint módszer, de kitûnôen
elkülöníthetôk vele a világszer- te … identifikáltuk [5, 6]. A mycobacteriális DNS kinyerését és a
spoligotipizálást ke- reskedelmi forgalomban kapható teszttel (Isogen Bioscience BV …
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FS Spies – 2007 – lume.ufrgs.br
… 48 3.18. Genotipagem dos isolados ….. 48 3.18.1.
Spoligotyping ….. 49 3.18.1.1. Primers….. 49 3.18.1.2. …
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MDB Fernandes – 2007 – bdtd.cict.fiocruz.br
… 32 Figura 2.8: Representação esquemática do spoligotyping. 35 Figura 2.9: Descrição do
spoligotyping de linhagens das maiores famílias do Mycobacterium tuberculosis. 36 Figura 2.10:
Representação esquemática da seqüência replicada utilizando DRE-PCR. …
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OIE de Salud Animal – inforural.com.mx
Page 1. 54 (Segunda Sección) DIARIO OFICIAL Lunes 3 de diciembre de 2007
SECRETARIA DE AGRICULTURA, GANADERIA, DESARROLLO RURAL, PESCA
Y ALIMENTACION PROYECTO de Modificación a la Norma …
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FS Spies – 2007 – lume.ufrgs.br
… 48 3.18. Genotipagem dos isolados ….. 48 3.18.1.
Spoligotyping ….. 49 3.18.1.1. Primers….. 49 3.18.1.2. …
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MDB Fernandes – 2007 – bdtd.cict.fiocruz.br
… 32 Figura 2.8: Representação esquemática do spoligotyping. 35 Figura 2.9: Descrição do
spoligotyping de linhagens das maiores famílias do Mycobacterium tuberculosis. 36 Figura 2.10:
Representação esquemática da seqüência replicada utilizando DRE-PCR. …
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2006
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A case of urinary tuberculosis due toMycobacterium bovissubspeciescaprae
V Sintchenko, P Jelfs, M Dally… – Pathology-Journal of …, 2006 – journals.lww.com
… Spoligotyping (Isogen Bioscience, The Netherlands), which involves PCR amplification of the
MTBC direct repeat … of reference laboratories to differentiate members of the MTBC by spoligotyping,
11 based on … Several spoligotypes have been recognised among M. bovis subsp. …
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C Allix, K Walravens, C Saegerman… – Journal of clinical …, 2006 – Am Soc Microbiol
… PCR products were detected by hybridization onto a spoligotyping membrane (Isogen Bioscience
BV … IS6110 RFLP, spoligotyping, and MIRU-VNTR patterns of isolates obtained from animals … based
on MIRU-VNTR types and IS6110 fingerprints and spoligotypes was evaluated …
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Z Hasan, M Tanveer, A Kanji, Q Hasan… – Journal of clinical …, 2006 – Am Soc Microbiol
… positive. Spoligotyping.All 314 isolates were spoligotyped and analyzed (Fig.
1). A total of 197 (63%) isolates were grouped into 22 different clusters, while
119 (37%) isolates had unique spoligotypes. Previously …
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B Romero, A Aranaz, L de Juan… – Journal of clinical …, 2006 – Am Soc Microbiol
… Spoligotyping was performed on a commercial membrane (Isogen Bioscience BV, Maarssen,
The … of techniques is required for appropriate discrimination, even for those spoligotypes that have
a … Furthermore, the design of a new spoligotyping membrane that complements the …
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N Suresh, UB Singh, J Arora, H Pant… – Infection, Genetics and …, 2006 – Elsevier
… to the AIIMS hospital, 149 MDR TB strains and 38 drug-susceptible strains were sequenced to
identify mutations associated with RIF r in the rpoB gene and spoligotyped. 2.2. … Spoligotyping.
A total of 80 distinct spoligotypes were obtained from 149 MDR M. tuberculosis isolates. …
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V Rasolofo Razanamparany, R Quirin… – Veterinary …, 2006 – Elsevier
… containing 43 oligonucleotides derived from spacer DNA sequences (obtained from Isogen
Bioscience BV, Utrecht, The Netherlands). PCR and hybridization were performed as described
by Kamerbeek et al. (1997). Spoligotyping patterns or spoligotypes were confirmed by …
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[HTML] Variantes genéticas de Mycobacterium tuberculosis aisladas de pacientes de la Xª Región de Chile
M Mancilla E, A Martínez H… – Revista chilena de …, 2006 – SciELO Chile
… A significant number of spoligotypes were identical to profiles found in Brazil followed by Argentina
and Spain. … utilizando los partidores biotinilados DRa (5′-GGTTTTGGGTCTGACGAC) y DRb (5′-
CCGAGA GGGGACGGAAAC), incluidos en el sistema de spoligotyping (ISOGEN. …
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[HTML] from oxfordjournals.org
BC de Jong, PC Hill, RH Brookes… – Journal of Infectious …, 2006 – jid.oxfordjournals.org
… were assessed by spectrophotometry (Eppendorf BioPhotometer). Spoligotyping
was performed using membranes (Isogen Biosciences), in accordance with
standardized methods [19]. Spoligotyping results were scanned and …
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X Ma, H Wang, Y Deng, Z Liu, Y Xu… – Journal of clinical …, 2006 – Am Soc Microbiol
… (20), with data analyzed by Whole Band Analysis software (version 3.2; BioImage,
Ann Arbor, MI). Spoligotyping was carried out by using commercial kits from Isogen
Bioscience BV (Maarssen, The Netherlands). Mycobacterial …
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YJ Sun, TK Lim, A Ong, B Ho… – BMC infectious …, 2006 – biomedcentral.com
… Spoligotyping was performed using a commercial kit (Isogen Bioscience BV, Maarssen, the
Netherlands … The remaining one isolate (N07) was not detected by spoligotyping, but MIRU-VNTR …
Based on spoligotypes and/or MIRU-VNTR patterns, the remaining 20 isolates were …
Cited by 29 – Related articles – Cached – All 22 versions – Import into BibTeX
P Farnia, MR Masjedi, M Mirsaeidi, F Mohammadi… – Journal of Infection, 2006 – Elsevier
… Spoligotyping. Spoligotyping was performed as described by Kamerbeek et al. 5 with a
commercially available kit, according to the instructions supplied by the manufacturer (Isogen
Bioscience BV, Maarsen, The Netherlands). … Distribution of the 263 MDR-TB spoligotypes. …
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Molecular epidemiology of drug-resistant tuberculosis in Hungary
C Ködmön, S Niemann, J Lukács… – Journal of clinical …, 2006 – Am Soc Microbiol
… described previously (3, 14, 17). Spoligotyping was performed with a commercially
available kit (Isogen Bioscience BV, Maarssen, The Netherlands) according to the
instructions of the manufacturer (10). The IS6110 fingerprint and …
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Tuberculosis in mandrills at the Lisbon zoo
A Amado, T Albuquerque, A Gonçalves… – Veterinary …, 2006 – veterinaryrecord.bmj.com
… In order to investigate the infection source, spoligotyping was performed using conventional 43
oligonucleotide Isogen membranes as described by Kamerbeek and others (1997). The two
isolates shared the same spoligotype described as type 237 (Anon 2006). …
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L Aristimuño, R Armengol, A Cebollada… – BMC …, 2006 – biomedcentral.com
… to a set of 43 oligonucleotides covalently bound to the spoligo-membrane (Isogen Biosciences
BV … of Mycobacterium tuberculosis and their global distribution assessed by spoligotyping in an
International study Global distribution of Mycobacterium tuberculosis spoligotypes. …
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I Sutcliffe, T Matsumoto, H Ano, T Nagai, K Danno… – 2006 – journals2005.pasteur.ac.ir
… Spoligotyping The clones of M. tuberculosis with less than five IS6110 RFLP bands were subjected
to the second typing, spoligotyping. Spoligotyping membranes were purchased from Isogen
bioscience BV (Biltho- ven, The Netherlands). The procedures were …
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M Caws, G Thwaites, K Stepniewska… – Journal of clinical …, 2006 – Am Soc Microbiol
… Spoligotyping.Spoligotyping of isolates was performed according to the standard protocol (6).
Briefly, DNA … Dra (biotin labeled) and DRb, hybridized to the spoligotype membrane (Isogen
Bioscience BV … Spoligotypes were available for isolates from 35 HIV-positive patients (Fig. …
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The most prevalent Mycobacterium tuberculosis superfamilies among Iranian and Afghan TB cases
AA Velayati, P Farnia, M Mirsaeidi… – … journal of infectious …, 2006 – informahealthcare.com
… [7] with a commercially available kit, according to the instructions supplied by the manufacturer
(Isogen Bioscience BV, Maarsen, The Netherlands). … Spoligotyping … Figure 1. Shows the
predominant spoligotypes which are prevalent among Iranian and Afghan-born immigrants. …
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Extensively drug-resistant tuberculosis: 2 years of surveillance in Iran
[HTML] from oxfordjournals.org
MR Masjedi, P Farnia, S Sorooch… – Clinical infectious …, 2006 – cid.oxfordjournals.org
… [13] with a commercially available kit (Isogen Bioscience). … On the basis of spoligotyping results,
these strains were determined to belong to the East African Indian 3 M. tuberculosis superfamily.
Table 1. Table 1 … Distribution of the 12 XDR TB spoligotypes. …
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[HTML] Predominance of ancestral lineages of Mycobacterium tuberculosis in India
MC Gutierrez, N Ahmed, E Willery… – Emerg Infect …, 2006 – journal.9med.net
… Spoligotyping was performed by using a commercial kit (Isogen Bioscience BV, Maarsen, the
Netherlands) according to … was built based on spoligotypes alone or on a combination of VNTR
and spoligotypes, although the resolution was lower when spoligotyping was used …
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High resolution typing system for pathogenic Mycobaterium tuberculosum
PS Keim, RS Spurgiesz, JM Schupp – US Patent 7,026,467, 2006 – Google Patents
… 7:390-6. 2001. Sola, C. et al., “Mycobacterium tuberculosis phylogeny reconstruction based on
combined numerical analysis with IS1081, IS6110, VNTR, and DR-based spoligotyping sug-
gests the existence of two new phlyogeographical clades”. J Molecr Ev. 53:680-9. 2001. …
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SUN YONGJIANG – 2006 – 137.132.14.55
… Furthermore, this method is highly reproducible and easy to perform (Kremer et al., 1999);
particularly spoligotypes can be digitized into binary or octal code formats (Dale et al., 2001)
which greatly facilitate the management and interlaboratory comparison of spoligotyping data. …
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Recent transmission of tuberculosis involving retired patients
PI Cafrune, LW Riley, LG Possuelo, ARM Valim… – Journal of Infection, 2006 – Elsevier
… Spoligotyping. Spoligotyping was performed as described by Kamerbeek et al. 19 …
probed. Hybridization was performed with a commercial kit, according to the
manufacturer instructions (Isogen Biosciences BV, The Netherlands). …
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[PDF] INFORME FINAL DE PROYECTO DE INVESTIGACIÓN
EN MULTIDROGORESISTENCIA… – bvs.ins.gob.pe
… Salud e interpretadas en el software ALFWin TM Sequence Analyser 2.0 1998. Ensayo
de genotipificación mediante Spoligotyping Fue usado un kit comercialmente
disponible (Isogen Bioscience BV, Maarssen, The Netherlands …
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A Aranaz, L De Juan, J Bezos, J Álvarez… – Veterinary …, 2006 – vetres.org
… Thirty-six M. bovis isolates were char- acterised by spoligotyping and MIRU- VNTR: the 23 M … The
biotin-labelled amplified product was hybridised onto a membrane (Isogen Bio- science BV,
Maarssen … Four spoligotypes were found, and three of them, numbered spb-7, spb-9 …
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Epidemiologia molecular da tuberculose resistente a múltiplos fármacos no Rio Grande do Sul
ARM Valim – 2006 – lume.ufrgs.br
… 72 4.6.4 Hibridização e Detecção….. 73 4.7 Spoligotyping ….. … 84 5.6 Análise dos
Padrões Gerados pela Genotipagem por Spoligotyping ….. 92 4.7. …
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EPIDEMIOLOGIA MOLECULAR DA TUBERCULOSE RESISTENTE A MÚLTIPLOS FÁRMACOS NO RIO GRANDE DO SUL
ARM Valim – 2006 – lume.ufrgs.br
… 72 4.6.4 Hibridização e Detecção….. 73 4.7 Spoligotyping ….. … 84 5.6 Análise dos
Padrões Gerados pela Genotipagem por Spoligotyping ….. 92 4.7. …
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INTÉRÊT DU TYPAGE MOLÉCULAIRE POUR L’ÉPIDÉMIOLOGIE DE LA TUBERCULOSE EN LIMOUSIN (1998–2006)
H Bezanahary, MC Baclet, C Sola… – Médecine et Maladies …, 2008 – Elsevier
… Keywords: MIRU15 typing; Mycobacterium tuberculosis; Spoligotyping. … L’hybridation (1H à 60
°C) des amplicons a été réalisée sur une membrane Isogen ® (Bioscience BV … sous forme d’un
code octal et comparés aux données de la banque de spoligotypes SpolDB4 (Institut …
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C Allix, K Walravens, C Saegerman… – Journal of clinical …, 2006 – Am Soc Microbiol
… PCR products were detected by hybridization onto a spoligotyping membrane (Isogen Bioscience
BV … IS6110 RFLP, spoligotyping, and MIRU-VNTR patterns of isolates obtained from animals … based
on MIRU-VNTR types and IS6110 fingerprints and spoligotypes was evaluated …
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A CASE OF URINARY TUBERCULOSIS DUE TOMYCOBACTERIUM BOVISSUBSPECIESCAPRAE
V Sintchenko, P Jelfs, M Dally… – Pathology-Journal of …, 2006 – journals.lww.com
… Spoligotyping (Isogen Bioscience, The Netherlands), which involves PCR amplification of the
MTBC direct repeat … of reference laboratories to differentiate members of the MTBC by spoligotyping,
11 based on … Several spoligotypes have been recognised among M. bovis subsp. …
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Z Hasan, M Tanveer, A Kanji, Q Hasan… – Journal of clinical …, 2006 – Am Soc Microbiol
… positive. Spoligotyping.All 314 isolates were spoligotyped and analyzed (Fig.
1). A total of 197 (63%) isolates were grouped into 22 different clusters, while
119 (37%) isolates had unique spoligotypes. Previously …
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B Romero, A Aranaz, L de Juan… – Journal of clinical …, 2006 – Am Soc Microbiol
… Spoligotyping was performed on a commercial membrane (Isogen Bioscience BV, Maarssen,
The … of techniques is required for appropriate discrimination, even for those spoligotypes that have
a … Furthermore, the design of a new spoligotyping membrane that complements the …
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N Suresh, UB Singh, J Arora, H Pant… – Infection, Genetics and …, 2006 – Elsevier
… to the AIIMS hospital, 149 MDR TB strains and 38 drug-susceptible strains were sequenced to
identify mutations associated with RIF r in the rpoB gene and spoligotyped. 2.2. … Spoligotyping.
A total of 80 distinct spoligotypes were obtained from 149 MDR M. tuberculosis isolates. …
Cited by 22 – Related articles – All 7 versions – Import into BibTeX
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[HTML] Spoligotyping of Mycobacterium bovis isolates found in Manitoba
C Lutze-Wallace, C Turcotte… – Canadian journal of …, 2005 – ncbi.nlm.nih.gov
… Table I. Table I Summary of spoligotyped isolates by year, species, and location. … types are
656573377603600 (MB-1) and 656473377603600 (MB-2). These spoligotypes have not …
Spoligotyping is particularly useful for differentiating isolates harbouring only a single copy of …
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M Gazouli, J Ikonomopoulos… – Journal of clinical …, 2005 – Am Soc Microbiol
… hybridization with membrane-bound spacer oligonucleotides and a spoligotyping kit (ISOGEN
Bioscience, Maarsen … The spoligotyping results were entered in a binary format as Excel
spreadsheets … All of the identified spoligotypes have been submitted to the World Spoligotype …
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K Kremer, BKY Au, PCW Yip, R Skuce… – Journal of clinical …, 2005 – Am Soc Microbiol
… In addition to spoligotyping with a commercial membrane containing the standard set of 43 spacer
oligonucleotides (Isogen Bioscience BV, Maarssen, The … (35), who also subjected Beijing strains
to spoligotyping with novel spacers, at least seven spoligotypes can be …
Cited by 64 – Related articles – All 17 versions – Import into BibTeX
DG de Viedma, NA Rodriguez, S Andrés… – Journal of clinical …, 2005 – Am Soc Microbiol
… Spoligotyping.Spoligotyping was performed using primers of a spoligotyping kit (Isogen, The
Netherlands … with the loci showing different alleles within a box) and spoligotypes for the … The two
clones with different MIRU types were indistinguishable by spoligotyping and RFLP (Fig …
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RA Skuce, SW McDowell, TR Mallon… – Veterinary …, 2005 – veterinaryrecord.bmj.com
… The isolates were spoligotyped according to the instructions of the manu- facturer (Isogen). … the
inclusion of more than one isolate per herd (VNTR D=0·90, spoligotyping D=0 … The difference
between years in the prevalence of VNTR pro- files and spoligotypes was not statistically …
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[HTML] Molecular genotyping of Mycobacterium tuberculosis isolates from Turkey
B Gencer… – Am J Infect Dis, 2005 – biomedsearch.com
… Of the 374 isolates spoligotyped, 87 (23%) displayed unique spoligotypes and 287 (77 …
Spoligotyping: Spoligotyping was performed using a commercial kit (Isogen Bioscience BV,
Maarssen … Spoligotyping: Spoligotypes were obtained for 374 isolates (Table 2): 87 isolates (23 …
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[CITATION] Epidemiologische Studie bei 109 Tuberkulosefällen im Regierungsbezirk Oberfalz basierend auf Spoligotyping
B Werner, C Ellermeier, L Naumann… – Pneumologie, 2005
C Schewe, T Goldmann, M Grosser, A Zink… – Virchows Archiv, 2005 – Springer
… All samples with positive amplification of IS6110 were subjected to further analysis by
spoligotyping. The method was performed as described previously [22, 32, 44]. We used a
commercial Spoligotyping Kit (Isogen, Maarsen, The Netherlands, http://www.isogen.nl/). …
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[CITATION] Case-Report: Lungentuberkulose einer MTA–Nachweis von 3 Neuinfektionen durchSpoligotyping
B Werner, C Ellermeier, L Naumann… – Pneumologie, 2005
S Borsuk, MM Dellagostin, SG Madeira, C Lima… – Microbes and …, 2005 – Elsevier
… Hybridization of the PCR product to the spoligo-membrane (Isogen Life Sciences) was … The strains
clustered by spoligotyping were analyzed by IS6110-RFLP to confirm any … The spoligotypes were
compared to SpoligoDatabase3 (http://www.pasteur-guadeloupe.fr/tb/spoldb3 …
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T Matsumoto, H Ano, T Nagai, K Danno… – Tuberculosis, 2005 – Elsevier
… to the second typing, spoligotyping. Spoligotyping membranes were purchased
from Isogen bioscience BV (Bilthoven, The Netherlands). The procedures were
carried out according to the manufacturer’s instructions. Results. …
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Distribution of the Beijing family genotypes of Mycobacterium tuberculosis in Taiwan
R Jou, CY Chiang… – Journal of clinical microbiology, 2005 – Am Soc Microbiol
… Genotyping method.Spoligotyping was used to identify and differentiate the M. tuberculosis
complex isolates in … A commercially available kit (Isogen Bioscience BV, Maarssen, The
Netherlands) was used as … The spoligotypes resolved were M. tuberculosis complex strain specific …
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WM Prodinger, A Brandstätter… – Journal of clinical …, 2005 – Am Soc Microbiol
… Spoligotyping and IS6110 RFLP analysis.Spoligotyping was carried out as described previously
(23) by using membranes (Isogen Bioscience BV, Maarsen, The Netherlands). The octal codes
for spoligotypes were determined as proposed previously (14). …
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S Davis, V Barros, C Portugal… – Rev Port …, 2005 – repositorio.hff.min-saude.pt
… out in accordance with the recommendations of the mem- brane Kit manufacturer (Isogen
Bioscience BV … contributed the most in this field, seeing that it allows the Spoligotypes to be …
Spoligotyping make it easier to differentiate between the species of the M. tuberculosis complex …
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I Mokrousov, O Narvskaya… – Journal of clinical …, 2005 – Am Soc Microbiol
… in parallel slots (channels) by using an MN45 miniblotter apparatus (Isogen Biosciences,
Maarssen … both ribotypes of the epidemic clone, while the other 33 spoligotypes included strains …
had unique profiles (Table 2). The discriminatory power of the spoligotyping assessed with …
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[PDF] 结核分枝杆菌散在分布重复单位基因型分型法的应用研究
沈国妙， 查佳， 徐琳， 孙斌， 桂晓红， 王奕峰… – 中华结核和呼吸 …, 2005 – lung.org.cn
… 3. Spoligotyping 基因分型方法：使用荷兰Isogen Bioscience 公司Spoligotyping 试剂盒并按照厂商提
供的使用手册进行操作。以10 ng 左右的DNA 作 为模板，PCR 法扩增间隔序列。PCR 产物与固定在
膜上的短核苷酸序列杂交，用ECL 系统检测杂交信 号。Spoligotyping …
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F Jauréguy, MC Gutierez, CJ Marie, C Poirier… – Pathologie Biologie, 2005 – Elsevier
… Keywords: Nosocomial tuberculosis; Epidemiology; Spoligotyping. … inter-DR ou spacers ont été
synthétisées et immobilisées sur une membrane de nylon commercialisée (Isogen Bioscience
BV, BT … Les résultats des différents spoligotypes obtenus sont présentés dans la Fig. …
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[CITATION] 波兰肺结核患者耐药结核分枝杆菌分子流行病学研究
A Sajduda, A Brzostek, M PopXawska, N Rastogi… – 结核与肺部疾病杂志, 2005
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LX Nouvel – 2005 – oatao.univ-toulouse.fr
… 61 FIGURE 14 : CARTE DES 41 LOCI MIRU SUR LE CHROMOSOME DE M.
TUBERCULOSIS H37RV ….. 63 FIGURE 15 : DENDROGRAMME DES 79 PROFILS
DE SPOLIGOTYPES DIFFERENTS DES 284 SOUCHES TYPEES. …
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[PDF] Editorial board• EDITORIAL BOARD
T Bhatnagar, S Boutaiba… – FEMS …, 2005 – journals2005.pasteur.ac.ir
FEMS MICROBIOLOGY LETTERS Volume 248, Issue 2, Pages 133-274 (15 July 2005) 1. Editorial
board• EDITORIAL BOARD Pages iii-vi Research Letters 2. Lipolytic activity from Halobacteria:
Screening and hydrolase production• SHORT COMMUNICATION Pages 133-140 …
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[HTML] SPOLIGOTYPING OF MYCOBACTERIUM BOVIS ISOLATES FOUND IN MANITOBA
C Lutze-Wallace, C Turcotte… – Canadian journal of …, 2005 – ncbi.nlm.nih.gov
… Table I. Table I Summary of spoligotyped isolates by year, species, and location. … types are
656573377603600 (MB-1) and 656473377603600 (MB-2). These spoligotypes have not …
Spoligotyping is particularly useful for differentiating isolates harbouring only a single copy of …
Cited by 12 – Related articles – All 7 versions – Import into BibTeX
M Gazouli, J Ikonomopoulos… – Journal of clinical …, 2005 – Am Soc Microbiol
… hybridization with membrane-bound spacer oligonucleotides and a spoligotyping kit (ISOGEN
Bioscience, Maarsen … The spoligotyping results were entered in a binary format as Excel
spreadsheets … All of the identified spoligotypes have been submitted to the World Spoligotype …
Cited by 14 – Related articles – BL Direct – All 15 versions – Import into BibTeX
K Kremer, BKY Au, PCW Yip, R Skuce… – Journal of clinical …, 2005 – Am Soc Microbiol
… In addition to spoligotyping with a commercial membrane containing the standard set of 43 spacer
oligonucleotides (Isogen Bioscience BV, Maarssen, The … (35), who also subjected Beijing strains
to spoligotyping with novel spacers, at least seven spoligotypes can be …
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LX Nouvel – 2005 – oatao.univ-toulouse.fr
… 61 FIGURE 14 : CARTE DES 41 LOCI MIRU SUR LE CHROMOSOME DE M.
TUBERCULOSIS H37RV ….. 63 FIGURE 15 : DENDROGRAMME DES 79 PROFILS
DE SPOLIGOTYPES DIFFERENTS DES 284 SOUCHES TYPEES. …
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[CITATION] CASE-REPORT: LUNGENTUBERKULOSE EINER MTA–NACHWEIS VON 3 NEUINFEKTIONEN DURCHSPOLIGOTYPING
B Werner, C Ellermeier, L Naumann… – Pneumologie, 2005
[PDF] EDITORIAL BOARD• EDITORIAL BOARD
T Bhatnagar, S Boutaiba… – FEMS …, 2005 – journals2005.pasteur.ac.ir
FEMS MICROBIOLOGY LETTERS Volume 248, Issue 2, Pages 133-274 (15 July 2005) 1. Editorial
board• EDITORIAL BOARD Pages iii-vi Research Letters 2. Lipolytic activity from Halobacteria:
Screening and hydrolase production• SHORT COMMUNICATION Pages 133-140 …
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2004
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R Kotłowski, IC Shamputa, NA El Aila… – Journal of clinical …, 2004 – Am Soc Microbiol
… In experimental studies, 27 M. tuberculosis isolates were analyzed. Ten colonies isolated from
one sputum sample were subjected to spoligotyping (Isogen; Bioscience, Maarssen, The
Netherlands) and IS6110-restriction fragment length polymorphism (RFLP) assay (13). …
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R Kotlowski, IC Shamputa, NA El Aila… – JOURNAL OF …, 2004 – ukpmc.ac.uk
… In experimental studies, 27 M. tuberculosis isolates were analyzed. Ten colonies isolated from
one sputum sample were subjected to spoligotyping (Isogen; Bioscience, Maarssen, The
Netherlands) and IS6110-restriction fragment length polymorphism (RFLP) assay (13). …
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Molecular fingerprinting of Mycobacterium bovis subsp. caprae isolates from Central Europe
W Erler, G Martin, K Sachse… – Journal of clinical …, 2004 – Am Soc Microbiol
… method (12) and subjected to direct-repeat-specific PCR and hybridization with membrane-bound
spacer oligonucleotides and a spoligotyping kit (ISOGEN Bioscience, Maarsen, The … Spoligotyping
of the 79 isolates revealed 11 different spoligotypes (designated C1 to …
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DG de Viedma, M Marín, MJ Ruiz… – Journal of clinical …, 2004 – Am Soc Microbiol
… The PCR for amplification of the DR region was performed with the primers in the
spoligotyping kit (Isogen, Maarsen, The Netherlands) and according to the instructions of
the manufacturer. All spoligotypes were confirmed by repeating the assay. …
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A Aranaz, B Romero, N Montero… – Journal of clinical …, 2004 – Am Soc Microbiol
… The test was carried out with membranes obtained from Isogen Bioscience BV, Maarssen, The …
associated with the DR locus and their influence on the interpretation of spoligotyping. …
corresponding to relapses or infections at different sites showed identical spoligotypes (6, 10, …
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Bovine tuberculosis (Mycobacterium bovis) in wildlife in Spain
A Aranaz, L De Juan, N Montero… – Journal of Clinical …, 2004 – Am Soc Microbiol
… of the biotin-labeled PCR product onto a spoligotyping membrane (Isogen Bioscience BV …
Schematic representation of spoligotyping patterns identified among wildlife and areas where
the patterns … Six spoligotypes were identified among the isolates from Doñana National Park. …
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[PDF] Simultaneous detection and typing of Mycobacterium tuberculosis complex bacteria
M de Boer, A van der Zanden… – cli-online.com
… of the mem- brane or by specific imaging systems developed by Isogen Life Science … in the
laboratory, because different M. tuberculosis strains or DNA can yield different spoligotypes. Thus
spoligotyping can be used for quality control of microscopy of AFB, cultures or nucleic …
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Transmission of tuberculosis from smear negative patients: a molecular epidemiology study
E Hernandez-Garduno, V Cook, D Kunimoto… – Thorax, 2004 – thorax.highwire.org
… A spoligotyping kit (Isogen Bioscience, Maarssen) was used as a secondary typing
technique for strains with five copies or less of IS6110. 13 Smear specimens were
stained with Auramine O and confirmed by Ziehl Neelsen staining. …
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YJ Sun, ASG Lee, ST Ng, S Ravindran… – Journal of clinical …, 2004 – Am Soc Microbiol
… Spoligotyping.Spoligotyping was performed using a commercial kit (Isogen Bioscience BV,
Maarssen, The Netherlands) according to the instructions of the manufacturer. The spoligotypes
were presented by using pseudo-original patterns or 15 octal codes, as described by …
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IC Shamputa, L Rigouts, LA Eyongeta… – Journal of clinical …, 2004 – Am Soc Microbiol
… Spoligotyping.Spoligotyping was performed with a commercial kit (Isogen Bioscience BV,
Maarssen, The … All 10 clones from each of the 97 patients were tested by spoligotyping. … of the 97
patients tested, including the 7 Beijing genotype strains, showed identical spoligotypes. …
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Relating the size of molecularly defined clusters of tuberculosis to the duration of symptoms
[HTML] from oxfordjournals.org
TP Giordano, H Soini, LD Teeter… – Clinical infectious …, 2004 – cid.oxfordjournals.org
… BioImage). Isolates containing <5 IS6110 elements were spoligotyped [27] with a
commercially available kit (Isogen Bioscience BV). Spoligotyping is an alternative
method for molecular characterization of M. tuberculosis isolates. …
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A Sajduda, A Brzostek, M Poplawska… – … of Tuberculosis and …, 2004 – ingentaconnect.com
… Spoligotyping was per- formed using a commercially available kit (Isogen Bioscience BV, Maarssen,
The Netherlands) accord- ing to the … In accordance with previous findings, spoligotyping was less
discrimina- tory than IS6110 RFLP.8,9 A total of 91 spoligotypes …
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[CITATION] КРАТКИЕ СООБЩЕНИЯ. СПОЛИГОТИПИРОВАНИЕ КУЛЬТУР МИКОБАКТЕРИЙ ТУБЕРКУЛЕЗА, ВЫДЕЛЕННЫХ ОТ БОЛЬНЫХ ИЗ САМАРСКОЙ …
Е Желткова, Я Балабанова… – Zhurnal …, 2004 – Redaktsiia zhurnala” Zhurnal …
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D Kunimoto, K Sutherland… – … of Tuberculosis and …, 2004 – ingentaconnect.com
… Two or more isolates with the same DNA fingerprint were called a cluster. A
spoligotyping kit (Isogen Bioscience, Maarssen, The Netherlands) was used for
secondary analysis of all strains with five copies or less of IS6110.6 …
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Species differentiation and antibiotic susceptibility testing with DNA microarrays
[PDF] from aseanbiotechnology.info
G Vernet, C Jay, M Rodrigue… – Journal of applied …, 2004 – Wiley Online Library
… (Please refer to Fig. 3a.). 4.2.1 Reference spoligotyping. (Please refer to Fig. … (1997). Reagents
were provided by Isogen Life Science (The Netherlands). … 4.2.2 Comparison of spoligotypes
obtained by dot-blot and by the microarray with a strain of M. bovis. (Please refer to Fig. …
Cited by 21 – Related articles – BL Direct – All 9 versions – Import into BibTeX
PJ Easterbrook, A Gibson, S Murad… – Journal of clinical …, 2004 – Am Soc Microbiol
… primer 5′-CCAAGAGGGGACGGAAAC-3′ and biotinylated primer 5′-GGTTTTGGGTCTG
ACGAC-3′). The PCR products were then hybridized to a Biodyne C membrane (Isogen
Bioscience, Maarsen … By using spoligotyping alone, 54 distinct spoligotypes were identified …
Cited by 37 – Related articles – BL Direct – All 12 versions – Import into BibTeX
Identification of MDR-TB Beijing/W and other Mycobacterium tuberculosis genotypes in Nairobi, Kenya
WA Githui, AM Jordaan, ES Juma… – … of Tuberculosis and …, 2004 – ingentaconnect.com
… Spoligotyping and analysis The method is based on in vitro PCR amplification of the … Membranes
and primers were obtained from Isogen, Bioscience BV, Utrecht, The Netherlands. … were scanned
and added to an existing spoligotype data- base containing spoligotypes from all …
Cited by 27 – Related articles – BL Direct – All 10 versions – Import into BibTeX
First isolation of< i> Mycobacterium microti</i>(Llama-type) from a dog
L Deforges, HJ Boulouis, JL Thibaud… – Veterinary …, 2004 – Elsevier
… Definitive subspecies identification was established by spoligotyping (for “Spacer Oligotyping …
Easthill, NY, USA) sensitised with 43 aminolink–oligonucleotides, according to the procedures
recommended by the manufacturer of the oligonucleotides (Isogen Bioscience, Maarssen …
Cited by 15 – Related articles – All 5 versions – Import into BibTeX
Impact of drug resistance on fitness of Mycobacterium tuberculosis strains of the W‐Beijing genotype
OS Toungoussova, DA Caugant… – FEMS Immunology …, 2004 – Wiley Online Library
… 20,21]. Spoligotyping was performed by using a commercially available kit (Isogen
Bioscience BV, Maarssen, The Netherlands) according to the instructions supplied
by the manufacturer, as previously described [22]. M. tuberculosis …
Cited by 31 – Related articles – All 7 versions – Import into BibTeX
6 Molecular Epidemiology of Mycobacterium bow’s
RA SKUCE… – Tuberculosis, 2004 – books.google.com
… e Spoligotyping result of M. tuberculosis H37Rv and M. bovis field isolates (rows), A membrane …
Courtesy of Isogen Bioscience BV representative panels of M. tuberculosis complex bac- teria
(van … 2000) and relatively large databases of spoligotypes are being curated (Sola et al. …
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D Nguyen, P Brassard, D Menzies… – Journal of clinical …, 2004 – Am Soc Microbiol
… Spoligotyping (or spacer oligonucleotide typing) was done by using a PCR-based technique
which detects the presence or absence of 43 unique sequence spacers in the direct repeat
(DR) locus (Isogen Bioscience BV, Maarssen, The Netherlands) (20). …
Cited by 30 – Related articles – BL Direct – All 14 versions – Import into BibTeX
PM Parreiras, FCF Lobato, AP Alencar… – Memórias do Instituto …, 2004 – SciELO Brasil
… 2002), applied on the membrane using a Miniblotter MN45 (Isogen, the Netherlands) and after
1 min incubation at RT, the membrane was … This observation was confirmed by the generation
of M. bovis genotypes after submitting the isolates to spoligotyping (data not shown). …
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[PDF] Antimicrobial resistance among Campylobacter strains, United States, 1997–2001
A Gupta, JM Nelson, TJ Barrett, RV Tauxe… – Emerg Infect …, 2004 – wwwnc.cdc.gov
Page 130. We summarize antimicrobial resistance surveillance data in human and
chicken isolates of Campylobacter. Isolates were from a sentinel county study from
1989 through 1990 and from nine state health departments …
Cited by 137 – Related articles – View as HTML – BL Direct – All 14 versions – Import into BibTeX
I Mokrousov, NV Bhanu, PN Suffys… – Journal of …, 2004 – Elsevier
… Furthermore, the proposed method requires the same simple equipment as that used for
spoligotyping and permits simultaneous analysis of … Biodyne ®C membrane (Pall Gelman
Laboratory, Ann Arbor, MI, USA) using the MN45 miniblotter apparatus (Isogen Biosci., Maarssen …
Cited by 47 – Related articles – All 16 versions – Import into BibTeX
R Kotłowski, IC Shamputa, NA El Aila… – Journal of clinical …, 2004 – Am Soc Microbiol
… In experimental studies, 27 M. tuberculosis isolates were analyzed. Ten colonies isolated from
one sputum sample were subjected to spoligotyping (Isogen; Bioscience, Maarssen, The
Netherlands) and IS6110-restriction fragment length polymorphism (RFLP) assay (13). …
Cited by 7 – Related articles – All 7 versions – Import into BibTeX
DG de Viedma, M Marín, MJ Ruiz… – Journal of clinical …, 2004 – Am Soc Microbiol
… The PCR for amplification of the DR region was performed with the primers in the
spoligotyping kit (Isogen, Maarsen, The Netherlands) and according to the instructions of
the manufacturer. All spoligotypes were confirmed by repeating the assay. …
Cited by 20 – Related articles – BL Direct – All 11 versions – Import into BibTeX
R Kotlowski, IC Shamputa, NA El Aila… – JOURNAL OF …, 2004 – ukpmc.ac.uk
… In experimental studies, 27 M. tuberculosis isolates were analyzed. Ten colonies isolated from
one sputum sample were subjected to spoligotyping (Isogen; Bioscience, Maarssen, The
Netherlands) and IS6110-restriction fragment length polymorphism (RFLP) assay (13). …
Cited by 2 – Related articles – BL Direct – All 3 versions – Import into BibTeX
A Aranaz, B Romero, N Montero… – Journal of clinical …, 2004 – Am Soc Microbiol
… The test was carried out with membranes obtained from Isogen Bioscience BV, Maarssen, The …
associated with the DR locus and their influence on the interpretation of spoligotyping. …
corresponding to relapses or infections at different sites showed identical spoligotypes (6, 10, …
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BOVINE TUBERCULOSIS (MYCOBACTERIUM BOVIS) IN WILDLIFE IN SPAIN
A Aranaz, L De Juan, N Montero… – Journal of Clinical …, 2004 – Am Soc Microbiol
… of the biotin-labeled PCR product onto a spoligotyping membrane (Isogen Bioscience BV …
Schematic representation of spoligotyping patterns identified among wildlife and areas where
the patterns … Six spoligotypes were identified among the isolates from Doñana National Park. …
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TRANSMISSION OF TUBERCULOSIS FROM SMEAR NEGATIVE PATIENTS: A MOLECULAR EPIDEMIOLOGY STUDY
E Hernandez-Garduno, V Cook, D Kunimoto… – Thorax, 2004 – thorax.highwire.org
… A spoligotyping kit (Isogen Bioscience, Maarssen) was used as a secondary typing
technique for strains with five copies or less of IS6110. 13 Smear specimens were
stained with Auramine O and confirmed by Ziehl Neelsen staining. …
Cited by 67 – Related articles – BL Direct – All 12 versions – Import into BibTeX
YJ Sun, ASG Lee, ST Ng, S Ravindran… – Journal of clinical …, 2004 – Am Soc Microbiol
… Spoligotyping.Spoligotyping was performed using a commercial kit (Isogen Bioscience BV,
Maarssen, The Netherlands) according to the instructions of the manufacturer. The spoligotypes
were presented by using pseudo-original patterns or 15 octal codes, as described by …
Cited by 33 – Related articles – BL Direct – All 16 versions – Import into BibTeX
IC Shamputa, L Rigouts, LA Eyongeta… – Journal of clinical …, 2004 – Am Soc Microbiol
… Spoligotyping.Spoligotyping was performed with a commercial kit (Isogen Bioscience BV,
Maarssen, The … All 10 clones from each of the 97 patients were tested by spoligotyping. … of the 97
patients tested, including the 7 Beijing genotype strains, showed identical spoligotypes. …
Cited by 50 – Related articles – All 12 versions – Import into BibTeX
PJ Easterbrook, A Gibson, S Murad… – Journal of clinical …, 2004 – Am Soc Microbiol
… primer 5′-CCAAGAGGGGACGGAAAC-3′ and biotinylated primer 5′-GGTTTTGGGTCTG
ACGAC-3′). The PCR products were then hybridized to a Biodyne C membrane (Isogen
Bioscience, Maarsen … By using spoligotyping alone, 54 distinct spoligotypes were identified …
Cited by 37 – Related articles – BL Direct – All 12 versions – Import into BibTeX
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2003
A Kwara, R Schiro, LS Cowan… – Journal of clinical …, 2003 – Am Soc Microbiol
… Spoligotyping (Isogen Bioscience BV, Maarssen, The Netherlands) was performed in accordance
with previously published methods (8). Results were recorded as an octal code (4). MIRU loci
(10) were amplified with primers specific for sequences flanking each locus (Table 1 …
Cited by 107 – Related articles – BL Direct – All 11 versions – Import into BibTeX
Characterization of Mycobacterium tuberculosis complex DNAs from Egyptian mummies byspoligotyping
AR Zink, C Sola, U Reischl, W Grabner… – Journal of clinical …, 2003 – Am Soc Microbiol
… For this analysis, we used a commercial spoligotyping kit (Isogen, Maarsen, The Netherlands …
it has been shown that the absence of spacers 33 to 36 in the spoligotyping signatures of … absence
of spacers 33 to 36 (Table 4), most of the M. tuberculosis spoligotypes obtained from …
Cited by 120 – Related articles – BL Direct – All 11 versions – Import into BibTeX
Characterization of Finnish Mycobacterium tuberculosis isolates by spoligotyping
K Puustinen, M Marjamäki, N Rastogi… – Journal of clinical …, 2003 – Am Soc Microbiol
… Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen,
The Netherlands) according to the instructions of the manufacturer. … We analyzed 380 M.
tuberculosis isolates by spoligotyping and obtained a total of 138 spoligotypes. …
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Widespread pyrazinamide-resistant Mycobacterium tuberculosis family in a low-incidence setting
D Nguyen, P Brassard, J Westley… – Journal of clinical …, 2003 – Am Soc Microbiol
… IS6110 RFLP by Southern blotting (38) and spoligotyping (Isogen Bioscience BV) (26) were
performed using … the observation that the DNA fingerprints by both IS6110 RFLP and spoligotyping
are similar … approximately 2 to 3.2 years (13, 42) and that of spoligotypes is believed to …
Cited by 15 – Related articles – BL Direct – All 10 versions – Import into BibTeX
[HTML] from oxfordjournals.org
OS Toungoussova, A Mariandyshev… – Clinical infectious …, 2003 – cid.oxfordjournals.org
… Spoligotyping was performed using a commercially available spoligotyping kit (Isogen Bioscience
BV) in … were represented by 6 and 3 isolates each, and 4 spoligotypes comprised 2 … All isolates
with identical IS6110 RFLP patterns were found to be identical by spoligotyping. …
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RS Spurgiesz, TN Quitugua, KL Smith… – Journal of clinical …, 2003 – Am Soc Microbiol
… In a database of 3,319 spoligotypes from isolates collected in geographically distinct areas, seven
spoligotype … Advanced Nucleic Acids Technology Core Facility, San Antonio, Tex.) and
commercially available spoligotyping membranes (Isogen, Maarssen, The …
Cited by 46 – Related articles – BL Direct – All 9 versions – Import into BibTeX
The molecular epidemiology of tuberculosis in western Canada
JM FitzGerald, A Fanning… – … of Tuberculosis and …, 2003 – ingentaconnect.com
… All isolates matched as identical by computer were manually confirmed by visually comparing
the original autoradiographs. A spoligotyping kit (Isogen Bioscience, Maarssen, The Netherlands)
was used for secondary analysis of 90 strains with five copies or less of IS6110. …
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SN Niobe-Eyangoh, C Kuaban, P Sorlin… – Journal of clinical …, 2003 – Am Soc Microbiol
… (i) Spoligotyping.Spoligotyping was performed … as a probe to hybridize with 43 synthetic spacer
oligonucleotides covalently bound to a membrane (Isogen Biosciences BV … Spoligotypes were
analyzed with the Bionumerics program version 2.5 (Applied Maths, Kortrijk, Belgium). …
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Detection of pathogenic mycobacteria of veterinary importance
RA Skuce, MS Hughes, MJ Taylor… – METHODS IN MOLECULAR …, 2003 – Springer
… 2.5.3. Membrane-Based Analysis 1. Spoligotyping Kit (Isogen Bioscience BV, Maarssen,
Netherlands). 2. SSPE buffer (2X): 1X SSPE is 0.18 M HCl, 10 mM NaH2PO4, 1 mM EDTA.
To be supplemented with 0.1 % sodium dodecyl sulfate (SDS). …
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Analysis of multidrug-resistant Mycobacterium bovis from three clinical samples from Scotland
VM Hughes, R Skuce, C Doig… – … of Tuberculosis and …, 2003 – ingentaconnect.com
… from each isolate was subjected to spoligotyping,11 exactly as specified by the kit manufacturers
(Isogen Bioscience BV … linked as they shared spoligotyping and DRr-RFLP profiles. The DRr-RFLP
profiles and spoligotypes shared by Cases I/94 and II/98 have never previously …
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[PDF] ÇOĞUL DİRENÇLİ MYCOBACTERİUM TUBERCULOSİS İZOLATLARINDA SEKONDER İLAÇLARA DİRENÇ VE
E TAKİP – 2003 – uvt.ulakbim.gov.tr
… Spoligotyping için ticari kit kullanılmıştır (Isogen Bioscience BV, Maarssen, The Netherlands … in
Cuba outside of Havana, July 1994- June 1995: utility of spoligotyping versus IS6110 … BN, Kremer
K., Valetudie G. et al, Global distribution of Mycobacterium tuberculosis spoligotypes. …
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FE Malone, EC Wilson, JM Pollock… – Journal of Veterinary …, 2003 – Wiley Online Library
… Molecular strain typing was undertaken on 39 cattle and three sheep M. bovis isolates using
spoligotyping (Kamerbeek et al., 1997) exactly as directed by the manufacturer (Isogen Bioscience,
Netherlands) and a selection of six VNTR markers (Skuce and Neill, 2001). …
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SL Sampson, RM Warren, M Richardson… – Journal of …, 2003 – Am Soc Microbiol
… These isolates were chosen on the basis of spoligotypes, which indicated a deletion of …
Spoligotyping.DNA polymorphism in the DR locus was detected by spoligotyping according to
a … products were then hybridized to a commercially available membrane (Isogen Biosciences …
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Heterogeneity of Mycobacterium tuberculosis isolates in Yangon, Myanmar
S Phyu, R Jureen, T Ti, UR Dahle… – Journal of clinical …, 2003 – Am Soc Microbiol
… susceptibility test, niacin accumulation test, nitrate reduction test, and pyrazinamidase activity)
were performed for phenotyping (4). Spoligotyping was performed with a commercial kit as
recommended by the manufacturer (Isogen Bioscience BV, Maarssen, The Netherlands). …
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SV Ramaswamy, R Reich, SJ Dou… – Antimicrobial agents …, 2003 – Am Soc Microbiol
… Spoligotyping and major genetic group assignment.Low-copy-number isolates were subjected
to a secondary typing method by use of spoligotyping. This method was performed with a
commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands) according to …
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Apport épidémiologique du typage moléculaire des bacilles de la tuberculose
V Vincent… – Médecine et Maladies Infectieuses, 2003 – Elsevier
… à partir d’un lysat cellulaire utilisé comme source d’ADN, puis hybridée à une membrane sur
laquelle sont fixés les 43 séquences inter DR les plus fréquentes (la membrane est
commercialisée par Isogen Bioscience BV, Maarsen, Hollande) (Fig. 2). Le spoligotyping est …
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[CITATION] Multicenter Evaluation of Reverse Line Blot Assay to Predict Drug Resistance in Mycobacterium tuberculosis Clinical Isolates
I Mokrousov, NV Bhanu, PN Suffys, GV Kadival…
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A Kwara, R Schiro, LS Cowan… – Journal of clinical …, 2003 – Am Soc Microbiol
… Spoligotyping (Isogen Bioscience BV, Maarssen, The Netherlands) was performed in accordance
with previously published methods (8). Results were recorded as an octal code (4). MIRU loci
(10) were amplified with primers specific for sequences flanking each locus (Table 1 …
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CHARACTERIZATION OF FINNISH MYCOBACTERIUM TUBERCULOSIS ISOLATES BY SPOLIGOTYPING
K Puustinen, M Marjamäki, N Rastogi… – Journal of clinical …, 2003 – Am Soc Microbiol
… Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen,
The Netherlands) according to the instructions of the manufacturer. … We analyzed 380 M.
tuberculosis isolates by spoligotyping and obtained a total of 138 spoligotypes. …
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WIDESPREAD PYRAZINAMIDE-RESISTANT MYCOBACTERIUM TUBERCULOSIS FAMILY IN A LOW-INCIDENCE SETTING
D Nguyen, P Brassard, J Westley… – Journal of clinical …, 2003 – Am Soc Microbiol
… IS6110 RFLP by Southern blotting (38) and spoligotyping (Isogen Bioscience BV) (26) were
performed using … the observation that the DNA fingerprints by both IS6110 RFLP and spoligotyping
are similar … approximately 2 to 3.2 years (13, 42) and that of spoligotypes is believed to …
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CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX DNAS FROM EGYPTIAN MUMMIES BYSPOLIGOTYPING
AR Zink, C Sola, U Reischl, W Grabner… – Journal of clinical …, 2003 – Am Soc Microbiol
… For this analysis, we used a commercial spoligotyping kit (Isogen, Maarsen, The Netherlands …
it has been shown that the absence of spacers 33 to 36 in the spoligotyping signatures of … absence
of spacers 33 to 36 (Table 4), most of the M. tuberculosis spoligotypes obtained from …
Cited by 120 – Related articles – BL Direct – All 11 versions – Import into BibTeX
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2002
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DG de Viedma, M Marin, S Hernangomez… – Archives of internal …, 2002 – Am Med Assoc
… The polymerase chain reaction (PCR) for amplifying the directed repeats region was performed
using primers of the spoligotyping kit (Isogen Bioscience, Maarssen, the Netherlands), following
the manufacturer’s instructions. All spoligotypes showing differences for the strains …
Cited by 65 – Related articles – Cached – BL Direct – All 5 versions – Import into BibTeX
[HTML] Molecular differentiation of Mycobacterium tuberculosis strains without IS6110 insertions
KH Lok, WH Benjamin Jr, ME Kimerling… – Emerging infectious …, 2002 – ncbi.nlm.nih.gov
… Spoligotyping membranes were purchased from Isogen Bioscience BV (Isogen, Bilthoven, the
Netherlands). … repeats results of three zero-copy IS6110 isolates with identical spoligotyping and
polymorphic … Fourteen of 32 spoligotyped strains are similar and have the same CDC …
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LS Cowan, L Mosher, L Diem… – Journal of clinical …, 2002 – Am Soc Microbiol
… Spoligotyping.Spoligotyping was performed using a commercial kit (Isogen Bioscience BV,
Maarssen, The … Q could also be divided by IS6110 RFLP and spoligotyping into subclusters
characterized by different insertion sites and divergent spoligotypes, as described …
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Infection of red deer, cattle, and humans with Mycobacterium bovis subsp. caprae in western Austria
WM Prodinger, A Eigentler… – Journal of clinical …, 2002 – Am Soc Microbiol
… on membranes with covalently bound, spacer sequence-specific oligonucleotides purchased
from Isogen Bioscience BV … region I isolates (3 cattle, 1 deer); lanes 5 to 9, spoligotypes from four …
wax-embedded tissue from that animal yielded the region II spoligotyping pattern (Fig. …
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Transmission of drug-resistant tuberculosis in Texas and Mexico
TN Quitugua, BJ Seaworth, SE Weis… – Journal of clinical …, 2002 – Am Soc Microbiol
… Locally synthesized and biotinylated PCR primers (Advanced Nucleic Acids Technology
Core Facility, UTHSCSA) were used with commercially available spoligotyping
membranes (Isogen, Maarssen, The Netherlands). Spoligotyping …
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OS Toungoussova, P Sandven… – Journal of clinical …, 2002 – Am Soc Microbiol
… Spoligotyping was performed by using a commercially available kit (Isogen Bioscience BV …
Spoligotyping.The spoligotyping method was used as an additional tool to further … to the Beijing
genotype by their IS6110 RFLP patterns showed identical spoligotypes, lacking spacers 1 …
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[HTML] Molecular typing of Mycobacterium tuberculosis strains with a common two-band IS6110 pattern
KH Lok, WH Benjamin Jr, ME Kimerling… – Emerging infectious …, 2002 – ncbi.nlm.nih.gov
… Spoligotyping membranes were used according to manufacturer’s recommendations (Isogen
Bioscience BV, Bilthoven … well between these sizes, which could result in different spoligotypes
of a … Using VNTR and spoligotyping techniques to reexamine some of these clusters, we …
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Sputum induction problems identified through genetic fingerprinting
B Nivin, T O’Flaherty, E Leibert, BY Zhao… – Infection control and …, 2002 – JSTOR
… Because spoligotyping is less discriminatory than IS6110-based analysis, identical spoligotypes
may indi- cate the need for further confirmation using the latter method. … The spoligotyping analysis
was performed using a commercially available kit (Isogen Bioscience BV …
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GE Thwaites, TTH Chau, M Caws… – … of Tuberculosis and …, 2002 – ingentaconnect.com
… was car- ried out in accordance with a standardised protocol for spoligotyping.16 Briefly … were
heat-denatured and hybridised to the spoligotype membrane (Isogen Biosciences, Asmaars …
Patterns were compared against the Beijing and ‘Vietnam’ spoligotypes described by Anh et …
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S Niemann, E Richter… – International journal of …, 2002 – Soc General Microbiol
… Spoligotyping, which is based on the detection of various non- repetitive spacer sequences located
between small repetitive units (direct … blotting technique, was performed as described previously
(Kamerbeek et al., 1997) by using a commercially available membrane (Isogen). …
Cited by 30 – Related articles – BL Direct – All 6 versions – Import into BibTeX
J Bruchfeld, G Aderaye, IB Palme… – Journal of clinical …, 2002 – Am Soc Microbiol
… synthetic spacer oligonucleotides which are covalently bound to a membrane (Isogen Bioscience
BV … 24, 41); and they all lacked the typical M. canetti spoligotyping patterns (presence of … 3.
Schematic representation of the spoligotypes of M. tuberculosis isolates belonging to four …
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[HTML] Genotyping analyses of tuberculosis cases in US-and foreign-born Massachusetts residents
S Sharnprapai, AC Miller, R Suruki… – Emerging infectious …, 2002 – ncbi.nlm.nih.gov
… with the molecular weight standards provided by CDC. Spoligotyping was performed
with a commercially available kit, in accordance with the manufacturer’s instructions
(Isogen Bioscience BV, Maarseen, the Netherlands). …
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WA Cronin, JE Golub, MJ Lathan… – Emerging Infectious …, 2002 – ncbi.nlm.nih.gov
… Spoligotyping was performed for all matching strains that had six or more IS6110
copies by using a commercially available kit, according to the manufacturer’s
instructions (Isogen Bioscience BV, Maarssen, the Netherlands) (13). …
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Q Eichbaum… – American Journal of Clinical …, 2002 – ajcp.ascpjournals.org
… Quentin Eichbaum, MD, PhD, MPH,1 and Eric J. Rubin, MD, PhD2 Key Words: Susceptibility;
Rapid culture; Molecular beacons; Nested PCR; Ligation; Spoligotyping; Strains DOI:
10.1309/XX1NBANX8JKMTUQN Page 2. Eichbaum and Rubin / TUBERCULOSIS …
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WA Cronin, JE Golub, MJ Lathan… – Emerging Infectious …, 2002 – ncbi.nlm.nih.gov
… Spoligotyping was performed for all matching strains that had six or more IS6110
copies by using a commercially available kit, according to the manufacturer’s
instructions (Isogen Bioscience BV, Maarssen, the Netherlands) (13). …
Cited by 2 – Related articles – All 3 versions – Import into BibTeX
S Niemann, E Richter… – International journal of …, 2002 – Soc General Microbiol
… Spoligotyping, which is based on the detection of various non- repetitive spacer sequences located
between small repetitive units (direct … blotting technique, was performed as described previously
(Kamerbeek et al., 1997) by using a commercially available membrane (Isogen). …
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2001
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Transmission of Mycobacterium tuberculosis to a Funeral Director During Routine Embalming*
M Lauzardo, P Lee, H Duncan… – Chest, 2001 – chestjournal.chestpubs.org
… Secondary strain typing of M tuberculosis was performed by spoligotyping (Isogen
Bioscience, BV; Maarssen, The Netherlands) as described by Kamerbeek et al. 10
Spacer regions within the direct repeat locus of the chromosome …
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WA Cronin, JE Golub, LS Magder… – Journal of clinical …, 2001 – Am Soc Microbiol
… Maryland culture-positive tuberculosis (TB) patients with low-copy IS6110-spoligotyped clustered
strains … investigation of low-copy clusters to those with rare spoligotypes may also … In conclusion,
spoligotyping did not appreciably improve strain differentiation among isolates with …
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Mycobacterium tuberculosis complex DNA from an extinct bison dated 17,000 years before the present
[HTML] from oxfordjournals.org
BM Rothschild, LD Martin, G Lev… – Clinical Infectious …, 2001 – cid.oxfordjournals.org
… The sequence was analyzed in an ABI PRISM 310 automated sequencer (PE Applied Biosystems).
Spoligotyping. To determine the species of Mycobacterium within the M. tuberculosis complex,
the spoligotyping system (Isogen) was applied in the Jerusalem laboratory. …
Cited by 113 – Related articles – BL Direct – All 12 versions – Import into BibTeX
Spread of drug-resistant pulmonary tuberculosis in Estonia
A Krüüner, SE Hoffner, H Sillastu… – Journal of clinical …, 2001 – Am Soc Microbiol
… Membranes for spoligotyping were obtained from Isogen, Bioscience BV, Utrecht, The Netherlands. …
Genetic homogenity of family A confirmed by spoligotyping.The 12 strains from family A showed
an … The spoligotypes of the non-Beijing family strains were highly diverse (Table 2 …
Cited by 160 – Related articles – BL Direct – All 10 versions – Import into BibTeX
H Soini, X Pan, L Teeter, JM Musser… – Journal of clinical …, 2001 – Am Soc Microbiol
… Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV,
Maarssen, The Netherlands) according to the instructions of the manufacturer. …
Spoligotyping.A total of 72 spoligotypes was identified among the 377 isolates. …
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WH Benjamin Jr, KH Lok, R Harris… – Journal of clinical …, 2001 – Am Soc Microbiol
… 36-bp direct repeats of M. tuberculosis, was done using membranes from Isogen Biosciences
BV … of direct repeat regions and thus loss of spacers on spoligotyping have been … The second report
explains differences between two common spoligotypes found in the IS6110 Harlem …
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Spoligotype diversity of Mycobacterium bovis strains isolated in France from 1979 to 2000
N Haddad, A Ostyn, C Karoui… – Journal of clinical …, 2001 – Am Soc Microbiol
… “None” corresponds to departments where two or more spoligotypes were codominant … (6), was
used for the spoligotyping of M … of 43 aminolink oligonucleotides by following the procedures
recommended by the manufacturer of the oligonucleotides (Isogen Bioscience, Maarssen …
Cited by 70 – Related articles – BL Direct – All 10 versions – Import into BibTeX
T Lillebaek, ÅB Andersen, J Bauer… – Journal of clinical …, 2001 – Am Soc Microbiol
… For spoligotyping, DNA extraction was performed by mechanical disruption of the cells with glass
beads by vortexing either a few colonies from solid media or 100 μl of the frozen culture stock
(27). The membranes used (Isogen; Bioscience BV, Utrecht, The Netherlands …
Cited by 132 – Related articles – BL Direct – All 11 versions – Import into BibTeX
TRANSMISSION OF MYCOBACTERIUM TUBERCULOSIS TO A FUNERAL DIRECTOR DURING ROUTINE EMBALMING*
M Lauzardo, P Lee, H Duncan… – Chest, 2001 – chestjournal.chestpubs.org
… Secondary strain typing of M tuberculosis was performed by spoligotyping (Isogen
Bioscience, BV; Maarssen, The Netherlands) as described by Kamerbeek et al. 10
Spacer regions within the direct repeat locus of the chromosome …
Cited by 9 – Related articles – BL Direct – All 5 versions – Import into BibTeX
WA Cronin, JE Golub, LS Magder… – Journal of clinical …, 2001 – Am Soc Microbiol
… Maryland culture-positive tuberculosis (TB) patients with low-copy IS6110-spoligotyped clustered
strains … investigation of low-copy clusters to those with rare spoligotypes may also … In conclusion,
spoligotyping did not appreciably improve strain differentiation among isolates with …
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MYCOBACTERIUM TUBERCULOSIS COMPLEX DNA FROM AN EXTINCT BISON DATED 17,000 YEARS BEFORE THE PRESENT
[HTML] from oxfordjournals.org
BM Rothschild, LD Martin, G Lev… – Clinical Infectious …, 2001 – cid.oxfordjournals.org
… The sequence was analyzed in an ABI PRISM 310 automated sequencer (PE Applied Biosystems).
Spoligotyping. To determine the species of Mycobacterium within the M. tuberculosis complex,
the spoligotyping system (Isogen) was applied in the Jerusalem laboratory. …
Cited by 113 – Related articles – BL Direct – All 12 versions – Import into BibTeX
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2000
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H Soini, X Pan, A Amin, EA Graviss… – Journal of clinical …, 2000 – Am Soc Microbiol
… Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen,
The … square analysis was used to test the association of clustered spoligotypes with IS6110 … The
sensitivity and specificity of spoligotyping were calculated by a method described …
Cited by 110 – Related articles – BL Direct – All 9 versions – Import into BibTeX
Use of spoligotype analysis to detect laboratory cross-contamination
B Nivin, J Driscoll, T Glaser, P Bifani… – Infection control and …, 2000 – JSTOR
… The spoligotyping analysis was performed using a commercially available kit (Isogen Bioscience
BV … In several cases of identical spoligotypes, the Wadsworth Center indicated that the …
Spoligotyping is an effective, relatively inexpensive (approximately 10% the cost of RFLP …
Cited by 12 – Related articles – BL Direct – All 6 versions – Import into BibTeX
S Niemann, E Richter… – Journal of clinical …, 2000 – Am Soc Microbiol
… sequences which were bound on a nylon membrane by reverse line blotting (Isogen, Maarssen,
The … PZA, and source of isolates.All isolates have been analyzed by spoligotyping and were …
comprising at least two isolates could be found (Fig.1). The spoligotypes among isolates …
Cited by 87 – Related articles – BL Direct – All 11 versions – Import into BibTeX
F Stauffer, A Makristathis, JP Klein… – Epidemiology and …, 2000 – Cambridge Univ Press
… se- quences of M. tuberculosis H37Rv and M. bo is BCG P3, being immobilized on a membrane
delivered by Isogen Bioscience (Maarssen … 1. Spoligotyping of MDR MTB strains isolated be-
tween 1995 and 1998 in Austria: Lanes 1 and 19 represent the spoligotypes of the …
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Principe et intéręt du typage moléculaire appliqué a Mycobacterium tuberculosis
V Vincent… – Revue Francaise des Laboratoires, 2000 – Elsevier
… La methode 26 spoligotyping repose sur la detection du polymorphisme des ces sequences
non conservees. … douteux (figure .2). La realisation de la methode est facilitee par la
commercialisation de membranes portent l’ensemble de ces 43 sequences (Isogen Bioscience …
Related articles – All 2 versions – Import into BibTeX
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1999
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J Bauer, ÅB Andersen, K Kremer… – Journal of clinical …, 1999 – Am Soc Microbiol
… culture stock) with glass beads by vortexing (26). Membranes for spoligotyping were
obtained from Isogen, Bioscience BV, Utrecht, The Netherlands. The membranes
contained oligonucleotides derived from the spacer DNA …
Cited by 126 – Related articles – BL Direct – All 9 versions – Import into BibTeX
DNA typing of a nonviable culture of Mycobacterium tuberculosis in a homeless shelter outbreak
JR Driscoll, MA McGarry… – Journal of clinical …, 1999 – Am Soc Microbiol
… Spoligotyping was performed on all the 1997 isolates from individuals involved in the outbreak,
and from a sample of nonviable cells from the 1992 culture of patient A, by using a commercially
available spoligotyping kit (Isogen, Maarssen, The Netherlands). …
Cited by 14 – Related articles – BL Direct – All 7 versions – Import into BibTeX
S Bonora, MC Gutierrez, G Di Perri… – Journal of clinical …, 1999 – Am Soc Microbiol
… (i) Spoligotyping.Spoligotyping was performed … probe to hybridize with 43 synthetic spacer
oligonucleotides attached to a carrier membrane (Isogen Bioscience BV … The software-assisted
evaluation of spoligotypes was done with GelCompar software, version 3.1b (Applied Maths …
Cited by 30 – Related articles – BL Direct – All 9 versions – Import into BibTeX
VS Perumaalla, LG Adams, J Payeur, D Baca… – Veterinary …, 1999 – Elsevier
… Spoligotyping was performed according to protocol described by the manufacturer (Isogen
Bioscience BV, Maarssen, The Netherlands). The method is based on amplification of the
polymorphic DR locus that is specific to organisms in the M. tuberculosis complex. …
Cited by 14 – Related articles – All 7 versions – Import into BibTeX
DNA typing of Mycobacterium bovis strains from the Castlepoint area of the Wairarapa
DM Collins – New Zealand Veterinary Journal, 1999 – Taylor & Francis
… For spoligotyping, filters containing the appropriate array of primers (Isogen Bioscience, The
Netherlands … 24 restriction types were differenti- ated into 11 PGRS types and 7 spoligotypes.
Combining the results of both PGRS typing and spoligotyping, dif- ferentiated the isolates …
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Extensive Cross-Contamination of Specimens withMycobacterium tuberculosis in a Reference Laboratory
MC Ramos, H Soini, GC Roscanni… – Journal of clinical …, 1999 – Am Soc Microbiol
… Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen,
The Netherlands … obtained between 8 May and 18 July 1995 were characterized by spoligotyping
and IS6110 … Seventeen different spoligotypes were identified among the 91M. …
Cited by 41 – Related articles – BL Direct – All 10 versions – Import into BibTeX
WH Haas, G Engelmann, B Amthor… – Journal of clinical …, 1999 – Am Soc Microbiol
… Spoligotyping.Spoligotyping was performed as previously described by Kamerbeek et al. (10). …
A total of 20 μl of the amplification product was hybridized with 43 oligonucleotides covalently
bound to a membrane (Isogen Bioscience BV … Analysis of spoligotypes was done by eye. …
Cited by 21 – Related articles – BL Direct – All 10 versions – Import into BibTeX
Evolution and clonal traits of Mycobacterium tuberculosis complex in Guinea-Bissau
G Källenius, T Koivula… – Journal of clinical …, 1999 – Am Soc Microbiol
… synthetic spacer oligonucleotides which are covalently bound to a membrane (Isogen Bioscience
BV … of copies of IS6110 elements, and frequently lack the spoligotyping spacers 33 … M.
tuberculosisisolates in terms of biochemical traits, RFLP patterns, and spoligotypes when they …
Cited by 60 – Related articles – BL Direct – All 9 versions – Import into BibTeX
MC Gutierrez, JC Galan, J Blazquez… – Journal of clinical …, 1999 – Am Soc Microbiol
… (v) Characterization of the DR region by spoligotyping.The DR cluster DNA of the strains was
amplified by PCR. The labeled PCR product was used as a probe to hybridize with 43 synthetic
spacer oligonucleotides attached to a carrier membrane (Isogen Bioscience BV), as …
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[PDF] The Molecular Epidemiology of Tuberculosis in Montreal and British Columbia
KA Musana – 1999 – nlc-bnc.ca
… 50 5.2.2 Results of Spoligotyping ….. 50 … 49 Table 7 . Surnmary of the results of spoligotyping
of isolates found to be identical but with 5 or less IS6110 bands ….. 51 …
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VS Perumaalla, LG Adams, J Payeur, D Baca… – Veterinary …, 1999 – Elsevier
… Spoligotyping was performed according to protocol described by the manufacturer (Isogen
Bioscience BV, Maarssen, The Netherlands). The method is based on amplification of the
polymorphic DR locus that is specific to organisms in the M. tuberculosis complex. …
Cited by 14 – Related articles – All 7 versions – Import into BibTeX
DNA TYPING OF MYCOBACTERIUM BOVIS STRAINS FROM THE CASTLEPOINT AREA OF THE WAIRARAPA
DM Collins – New Zealand Veterinary Journal, 1999 – Taylor & Francis
… For spoligotyping, filters containing the appropriate array of primers (Isogen Bioscience, The
Netherlands … 24 restriction types were differenti- ated into 11 PGRS types and 7 spoligotypes.
Combining the results of both PGRS typing and spoligotyping, dif- ferentiated the isolates …
Cited by 11 – Related articles – BL Direct – All 4 versions – Import into BibTeX
C Ködmön, S Niemann, MC Gutierrez… – Infection, Genetics and …, 2007 – Elsevier
… Spoligotyping was performed with a commercially available kit (Isogen Bioscience
BV, Maarssen, The Netherlands) according to the instructions of the manufacturer
(Kremer et al., 1999). Briefly, genomic mycobacterial DNA …
Cited by 5 – Related articles – All 5 versions – Import into BibTeX
WH Haas, G Engelmann, B Amthor… – Journal of clinical …, 1999 – Am Soc Microbiol
… Spoligotyping.Spoligotyping was performed as previously described by Kamerbeek et al. (10). …
A total of 20 μl of the amplification product was hybridized with 43 oligonucleotides covalently
bound to a membrane (Isogen Bioscience BV … Analysis of spoligotypes was done by eye. …
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SUN YONGJIANG – 2006 – 137.132.14.55
… Furthermore, this method is highly reproducible and easy to perform (Kremer et al., 1999);
particularly spoligotypes can be digitized into binary or octal code formats (Dale et al., 2001)
which greatly facilitate the management and interlaboratory comparison of spoligotyping data. …
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EVOLUTION AND CLONAL TRAITS OF MYCOBACTERIUM TUBERCULOSIS COMPLEX IN GUINEA-BISSAU
G Källenius, T Koivula… – Journal of clinical …, 1999 – Am Soc Microbiol
… synthetic spacer oligonucleotides which are covalently bound to a membrane (Isogen Bioscience
BV … of copies of IS6110 elements, and frequently lack the spoligotyping spacers 33 … M.
tuberculosisisolates in terms of biochemical traits, RFLP patterns, and spoligotypes when they …
Cited by 60 – Related articles – BL Direct – All 9 versions – Import into BibTeX
MC Gutierrez, JC Galan, J Blazquez… – Journal of clinical …, 1999 – Am Soc Microbiol
… (v) Characterization of the DR region by spoligotyping.The DR cluster DNA of the strains was
amplified by PCR. The labeled PCR product was used as a probe to hybridize with 43 synthetic
spacer oligonucleotides attached to a carrier membrane (Isogen Bioscience BV), as …
Cited by 31 – Related articles – BL Direct – All 9 versions – Import into BibTeX
[PDF] THE MOLECULAR EPIDEMIOLOGY OF TUBERCULOSIS IN MONTREAL AND BRITISH COLUMBIA
KA Musana – 1999 – nlc-bnc.ca
… 50 5.2.2 Results of Spoligotyping ….. 50 … 49 Table 7 . Surnmary of the results of spoligotyping
of isolates found to be identical but with 5 or less IS6110 bands ….. 51 …
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J Bauer, ÅB Andersen, K Kremer… – Journal of clinical …, 1999 – Am Soc Microbiol
… culture stock) with glass beads by vortexing (26). Membranes for spoligotyping were
obtained from Isogen, Bioscience BV, Utrecht, The Netherlands. The membranes
contained oligonucleotides derived from the spacer DNA …
Cited by 126 – Related articles – BL Direct – All 9 versions – Import into BibTeX
DNA TYPING OF A NONVIABLE CULTURE OF MYCOBACTERIUM TUBERCULOSIS IN A HOMELESS SHELTER OUTBREAK
JR Driscoll, MA McGarry… – Journal of clinical …, 1999 – Am Soc Microbiol
… Spoligotyping was performed on all the 1997 isolates from individuals involved in the outbreak,
and from a sample of nonviable cells from the 1992 culture of patient A, by using a commercially
available spoligotyping kit (Isogen, Maarssen, The Netherlands). …
Cited by 14 – Related articles – BL Direct – All 7 versions – Import into BibTeX
S Bonora, MC Gutierrez, G Di Perri… – Journal of clinical …, 1999 – Am Soc Microbiol
… (i) Spoligotyping.Spoligotyping was performed … probe to hybridize with 43 synthetic spacer
oligonucleotides attached to a carrier membrane (Isogen Bioscience BV … The software-assisted
evaluation of spoligotypes was done with GelCompar software, version 3.1b (Applied Maths …
Cited by 30 – Related articles – BL Direct – All 9 versions – Import into BibTeX
EXTENSIVE CROSS-CONTAMINATION OF SPECIMENS WITHMYCOBACTERIUM TUBERCULOSIS IN A REFERENCE LABORATORY
MC Ramos, H Soini, GC Roscanni… – Journal of clinical …, 1999 – Am Soc Microbiol
… Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen,
The Netherlands … obtained between 8 May and 18 July 1995 were characterized by spoligotyping
and IS6110 … Seventeen different spoligotypes were identified among the 91M. …
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1998
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TOWARDS A STANDARDIZED APPROACH TO DNA FINGERPRINTING OF MYCOBACTERIUM BOVIS
DV Cousins, RA Skuce, RR Kazwala… – … of Tuberculosis and …, 1998 – ingentaconnect.com
… in word processing program Perform PGRS RFLP on common Spoligotypes – use Alu I digest …
Spoligotyping A standard set of oligonucleotides for amplification of spacer regions as … A
commercially available kit (Isogen, Bioscience BV, The Netherlands), containing spoligotyping …
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DRUG RESISTANCE RATES OF MYCOBACTERIUM TUBERCULOSIS STRAINS IN AUSTRIA BETWEE
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2012
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A Ruettger, J Nieter, A Skrypnyk… – Journal of Clinical …, 2012 – Am Soc Microbiol
… Short-Form Paper 4 reverse line blot hybridization using the Spoligotyping Kit (Ocimum
Biosolutions, Hydera- 79 bad, India) and the present DNA microarray. … ArrayStrip spoligotyping
and the assay’s capability of identifying new spoligotypes and li- 109 neages. …
MI Millán-Lou, H Alonso, P Gavín… – Journal of Clinical …, 2012 – Am Soc Microbiol
… First, all 292 isolates were screened by spoligotyping with a commercially available
kit (Ocimum Biosolutions Ltd., Hyderabad, India) by the method of Kamerbeek et al.
(14). Seventy of the 292 isolates belonged to the Beijing family. …
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GenoType MTBDRsl performance on clinical samples with diverse genetic background
P Miotto, AM Cabibbe, P Mantegani… – European …, 2012 – Eur Respiratory Soc
… Spoligotyping. The amplified direct repeat locus of clinical isolates was investigated using the
Spoligotyping kit (Ocimum Biosolutions, Hyderabad, India) following the manufacturer’s
instructions. Spoligotyping results were entered, in binary format, in the MIRU-VNTRplus web …
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B Varghese, A Hillemann, DR Wijayanti… – Infection, Genetics and …, 2012 – Elsevier
… Of the 151 isolates, 146 with interpretable mutation results were subjected to MIRU-VNTR typing
and spoligotyping. Spoligotyping was performed as previously described (Kamerbeek et al., 1997)
with the commercial kit (Ocimum Biosolutions, Hyderabad, India). …
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O Kisa, G Tarhan, S Gunal, A Albay, R Durmaz… – PloS one, 2012 – dx.plos.org
… Spoligotyping was carried out with a commercial kit (Isogen Bioscience BV, Maarssen, The
Netherlands … tuberculosis H37Rv and M.bovis BCG P3 were used as control for spoligotyping.
Spoligotypes results were converted into octal code and in the SITVIT2 proprietary database …
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Y Pang, Y Zhou, B Zhao, G Liu, G Jiang, H Xia… – PloS one, 2012 – dx.plos.org
… A commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands) was used as
described by … of the direct repeat region of Mycobacterium tuberculosis: implications for
interpretation of spoligotyping data. … 2004) Predominant tuberculosis spoligotypes, Delhi, India. …
PM Parreiras, GI Andrade… – Memórias do Instituto …, 2012 – SciELO Brasil
… The PCR products were hybridised to a membrane (Isogen, The Netherlands) containing 43
oligonucleotides of known spacer sequences by reversed-line blot hybridisation. … Spoligotyping –
Complete spoligotypes were obtained from 61 isolates (Table I). In total, 17 …
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EVALUATION OF 24-LOCUS MIRU-VNTR IN EXTRAPULMONARY SPECIMENS: STUDY FROM A TERTIARY CENTRE IN MUMBAI
V Vadwai, A Shetty, P Supply… – Tuberculosis, 2012 – Elsevier
… Spoligotyping, was performed using a commercial kit (Isogen, Netherlands) as per the … 13/69,
18.8%) spoligo-prototypes, compared to LAM, Haarlem or T spoligotypes represented at … Analysis
of the congruence between MIRU-VNTR and spoligotyping results combined with the …
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V Di Marco, P Mazzone, MT Capucchio… – Journal of Clinical …, 2012 – Am Soc Microbiol
… direct repeat locus were detected by hybridization onto a spoligotyping membrane (Isogen 231 …
M. bovis isolates from both field studies were genotyped by both spoligotyping 342 … Four
spoligotypes were identified: SB0120 (23 isolates), SB0841 (five isolates), SB0833 (two 344 …
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TRANSMISSION OF TUBERCULOSIS WITHIN FAMILY-HOUSEHOLDS
E Augustynowicz-Kopeć, T Jagielski, M Kozińska… – Journal of Infection, 2012 – Elsevier
… Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen …
had different M. tuberculosis isolates, as evidenced by both spoligotyping and MIRU … Assuming
isolates revealing both identical spoligotypes and VNTR profiles are representing the …
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A Deribew, G Abebe, L Apers… – BMC Infectious …, 2012 – biomedcentral.com
… Standard spoligotyping method [12] was done generally as described by Kamerbeek and
colleagues using a commercially available kit (Isogen Bioscience BV … Spoligotypes described
only once (non-clustered) in this study and in the SpolDB4 were designated as …
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A Ayaz, Z Hasan, S Jafri, R Inayat, R Mangi… – International Journal of …, 2012 – Elsevier
… The spoligotypes were compared with the most prevalent MTB subfamilies as identified by the
World Spoligotyping Database SpolDB4.0 of the Pasteur Institute, Guadeloupe. 18. … 3.4. Genotypic
pattern of MTB isolates. Of the 1004 isolates, 987 were spoligotyped. …
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S David, EL Duarte, CQF Leite, JN Ribeiro… – Infection, Genetics and …, 2012 – Elsevier
… patient), were genotyped by spoligotyping according to the manufacturer’s instructions (Isogen
Bioscience BV … Contribution of Spoligotyping to the Characterization of the population structure
of … Update on the Spoligotypes of Mycobacterium tuberculosis complex isolates from the …
ÜO ZEYTİNLİ… – Mikrobiyol Bul, 2012 – mikrobiyolbul.org
… Key words: Mycobacterium tuberculosis; spoligotyping; MIRU-VNTR; molecular epidemiology;
Turkey. GİRİŞ … Spoligotiplendirme Spoligotiplendirme yöntemi, üretici firmanın önerileri
(Isogen Bioscience BV, Hollan- da) dikkate alınarak uygulandı. …
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2011
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W Waters, GE Stevens, MA Schoenbaum… – Veterinary medicine …, 2011 – hindawi.com
… Isolates of M. bovis were identified by a combination of Ziehl-Neelsen acid-fast staining, nucleic
acid probes (AccuProbe, Gen-Probe, San Diego, CA, USA), and spoligotyping (Ocimum
Biosolutions Ltd., Hyderabad, India). Identification of atypical Mycobacteria spp. …
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E Abadia, J Zhang, V Ritacco… – BMC infectious …, 2011 – biomedcentral.com
… of hard to interpret spoligotypes and samples representative of the work flow, so we could evaluate
the benefits of a new platform regarding the classical one on these samples. Most centers had
performed spoligotyping using commercial membranes (Ocimum, Hyderabad, India …
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PJ Freidlin, D Goldblatt, H Kaidar-Shwartz… – … and Quality Assurance: …, 2011 – Springer
… for spoli- gotyping results, standardization and reproducibility depend on the quality of the
commercial spoligotyping kit (Ocimum). … Spoligotyping Pattern … Both PETRA (a MIRU-VNTR lineage
with central asian spoligotypes, CAS) and Beijing clusters have been observed in other …
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S Purwar, S Chaudhari, VM Katoch… – Infection, Genetics and …, 2011 – Elsevier
… commercially available kit 126 (Isogen Biosciences , BV, Maarsen, The Netherlands now Ocimum
Biosolutions) and … Owing to small sample size, the exact prevalence of various 272 spoligotypes
and M … Out of the isolates clustered by spoligotyping, all 280 were unique strains by …
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B Varghese, A Hillemann, DR Wijayanti… – Infection, Genetics and …, 2012 – Elsevier
… Of the 151 isolates, 146 with interpretable mutation results were subjected to MIRU-VNTR typing
and spoligotyping. Spoligotyping was performed as previously described (Kamerbeek et al., 1997)
with the commercial kit (Ocimum Biosolutions, Hyderabad, India). …
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T Oni, R Burke, R Tsekela, N Bangani, R Seldon… – Thorax, 2011 – thorax.highwire.org
… Lowenstein–Jensen slope cultures. The DNA was used to perform spoligotyping
as previously described4 using a commercially available kit from Ocimum Biosolutions
BV, The Netherlands. The hybridisation patterns obtained …
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G Mamo, G Bayleyegn, TS Tessema, M Legesse… – PloS one, 2011 – dx.plos.org
… Spoligotyping. Spoligotyping was performed as previously described by Kamerbeek
and coauthors [22] and according to the spoligotype kit supplier’s instructions (Ocimum
Biosolutions Company, Iisselstein, The Netherlands). …
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POPULATION-BASED INVESTIGATION OF FLUOROQUINOLONES RESISTANT TUBERCULOSIS IN RURAL EASTERN CHINA
Y Hu, B Mathema, W Wang, B Kreiswirth, W Jiang… – Tuberculosis, 2011 – Elsevier
… Spoligotyping was carried out using commercial kits from Isogen Bioscience BV (Maarssen, The
Netherlands). 15 Strain family was determined by comparison of spoligotyping patterns with the
SpoligDB4 database and assigned a spoligotyping international type (SIT). …
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[HTML] THE GUINEA-BISSAU FAMILY OF MYCOBACTERIUM TUBERCULOSIS COMPLEX REVISITED
R Groenheit, S Ghebremichael, J Svensson… – PLoS One, 2011 – dx.plos.org
… All isolates that were subjected to RFLP were also spoligotyped. … Altogether, 414 isolates were
available for spoligotyping, of which 336 isolates were grouped into 42 clusters … Out of 229 isolates,
82 different spoligotypes were obtained, of which 168 (73.4%) were clustered into …
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GENETIC DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS IN MBARARA, SOUTH WESTERN UGANDA
J Bazira, M Matte, BB Asiimwe… – African health sciences, 2011 – ajol.info
… was performed as previously described29 using a commercially available kit (Isogen Bioscience
BV … Spoligotypes that did not match any existing pattern in the SpolDB4 database were … tuberculosis
within the Mbarara, we have used RD analysis and spoligotyping to characterize …
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Y Rafiq, K Jabeen, R Hasan, S Jafri… – Antimicrobial agents …, 2011 – Am Soc Microbiol
… was performed on all FQ-resistant strains using a commercially available kit provided by Isogen
Life Science BV … The spoligotypes were compared with the most prevalent M. tuberculosis
subfamilies, as identified by the world spoligotyping database, SpolDB4.0, of …
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[HTML] CHARACTERISTICS OF PNCA MUTATIONS IN MULTIDRUG-RESISTANT TUBERCULOSIS IN TAIWAN
YC Chiu, SF Huang, KW Yu, YC Lee… – BMC infectious …, 2011 – biomedcentral.com
… Genotyping. All clinical isolates were genotyped by a commercial spoligotyping kit (Isogen
Bioscience BV, Maarssen, Netherlands). The “Beijing strain” was defined as deletion from spacer
1 to spacer 34 in the direct repeat region and the presence of (at least 3) spacers 35-43. …
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JY Feng, WJ Su, YC Chiu, SF Huang, YY Lin… – PloS one, 2011 – dx.plos.org
… All clinical isolates were genotyped using a commercial spoligotyping kit (Isogen Bioscience
BV, Maarssen, Netherlands). The “Beijing strain” was defined as a deletion from spacer 1 to spacer
34 in the direct repeat region and the presence of (at least 3) spacers in 35–43. …
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[HTML] MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS, BUENOS AIRES, ARGENTINA
X Gonzalo, M Ambroggi, E Cordova… – Emerging Infectious …, 2011 – ncbi.nlm.nih.gov
… DNA was extracted from cultures at this UK laboratory; spoligotyping was performed according
to the manufacturer’s instructions (Isogen Life Science, IJsselstein, the Netherlands); and data
were analyzed with SPOTCLUST (http://cgi2.cs.rpi.edu/~bennek/SPOTCLUST.html). …
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CW Chang, MH Wu, PC Chuang… – Infection, Genetics and Evolution, 2011 – Elsevier
… 12 13 Spoligotyping 14 A commercially available kit (Isogen Bioscience BV, Maarssen, The 15
Netherlands) was used as … Even through spoligotyping results 3 revealed the genetic diversities
of MDR M. tuberculosis isolates in Taiwan, 4 identical spoligotypes could not …
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IF Padilla-Campos, JC Segura-Correa… – Tropical and …, 2011 – redalyc.uaemex.mx
… Key words: Bovine tuberculosis; Spoligotypes; M. bovis; Risk factors. … Spoligotyping was carried
out following the protocol described by Kamerbeek et al … sequences from M. tuberculosis and
six spacer sequences for M. bovis BCG were covalently bound (ISOGEN, Maarssen, The …
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[HTML] FINE-TUNING THE SPACE, TIME, AND HOST DISTRIBUTION OF MYCOBACTERIA IN WILDLIFE
C Gortazar, MJ Torres, P Acevedo, J Aznar… – BMC …, 2011 – biomedcentral.com
… Typing. The M. bovis isolates were further characterized by spoligotyping [40]. The amplified
product was detected by hybridization of the biotin-labelled PCR product onto spoligotyping
membrane (Isogen Bioscience BV, Maarssen, The Netherlands). …
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IF Padilla, JC Segura, JC Rodriguez… – Tropical and …, 2011 – ccba.uady.mx
… Key words: Bovine tuberculosis; Spoligotypes; M. bovis; Risk factors. … Spoligotyping was carried
out following the protocol described by Kamerbeek et al … sequences from M. tuberculosis and
six spacer sequences for M. bovis BCG were covalently bound (ISOGEN, Maarssen, The …
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K Faksri, F Drobniewski, V Nikolayevskyy, T Brown… – Tuberculosis, 2011 – Elsevier
… isolated from CSF samples collected from 1996 to 2004 with IS6110 RFLPs (120 RFLP patterns
were available) and spoligotyping results (141 spoligotypes were available … 27 Spoligotyping
was performed according to the manufacturer’s instructions (Isogen Bioscience BV …
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M Alonso, Y Navarro, F Barletta… – Clinical …, 2011 – Wiley Online Library
… Thirty-two MTB strains (18 representatives of the Beijing genotype and 14 representatives of
the non-Beijing genotype, according to their spoligotypes) were grown on … Spoligotyping was
performed in accordance with the manufacturer’s instructions (Isogen, De Meern, the …
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PJ Freidlin, D Goldblatt, H Kaidar-Shwartz… – … and Quality Assurance: …, 2011 – Springer
… 43 spacer spoligotyping was performed according to the manufacturer’s instructions
(Isogen/Ocimun) as described previously. … 27 Spoligotyping Pattern … Both PETRA (a MIRU-VNTR
lineage with central asian spoligotypes, CAS) and Beijing clusters have been observed in other …
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EPIDEMIOLOGICAL ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS STRAINS ISOLATED IN LODZ, POLAND
M Krawczyk, A Brzostek, A Gorna… – … of Tuberculosis and …, 2011 – ingentaconnect.com
… of van Embden et al.16 Spoligotyping was performed as described by Kamerbeek et al., using
the Isogen Life Science kit … Spoligotyping analysis showed that 184 (78.6%) of the strains were
grouped in clusters and 50 (21.4%) strains possessed unique spoligotypes. …
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U Bidovec-Stojkovic, M Zolnir-Dovc… – Respiratory Medicine, 2011 – Elsevier
… 4,9,20 Spoligotyping was performed using a commercially available kit (Isogen Bioscience BV …
identically defined by IS6110 RFLP and 24-locus MIRU-VNTR typing combined with spoligotyping. …
jointly defined by identical 24-locus MIRU-VNTR types and spoligotypes were only …
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Y Du, Y Qi, L Yu, J Lin, S Liu, H Ni… – Research in veterinary …, 2011 – Elsevier
… 2.5. Spoligotyping. Spoligotyping kits and Mini-blotter MN45 were obtained from Isogen
Life Science Co. (Utrecht, the Netherlands). The Streptavidin-ß-peroxidase conjugate was
produced by Roche Applied Science Co. (Penzberg, Germany). …
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Y Hu, B Mathema, W Jiang, B Kreiswirth, W Wang… – PloS one, 2011 – dx.plos.org
… Hybridized DNA was detected using an enhanced chemiluminescene kit from Isogen Bioscience
BV … b Probaiblity that the Spoligotyping pattern belongs to the clades. … N, Bennett KP (2006)
Identifying Mycobacterium tuberculosis complex strain families using spoligotypes. …
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SUBLINEAGES OF BEIJING STRAIN OF MYCOBACTERIUM TUBERCULOSIS IN SRI LANKA
US Rajapaksa… – Indian Journal of Microbiology, 2011 – Springer
… Spoligotyping was carried out according to manufacturer’s guidelines using a
commercially available kit (Isogen, Maarsen MV, The Netherland). Fourteen iso-
lates that confirmed to be Beijing strain were further characterised. …
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S Purwar, S Chaudhari, VM Katoch… – Infection, Genetics and …, 2011 – Elsevier
… 43 spacers was done with the help of a commercially available kit 126 (Isogen Biosciences ,
BV … Owing to small sample size, the exact prevalence of various 272 spoligotypes and M.
tuberculosis … Out of the isolates clustered by spoligotyping, all 280 were unique strains by IS6110 …
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MOLECULAR EPIDEMIOLOGY OF HUMAN AND ANIMAL TUBERCULOSIS IN IBADAN, SOUTHWESTERN NIGERIA
AO Jenkins, SIB Cadmus, EH Venter… – Veterinary …, 2011 – Elsevier
… Spoligotyping167 Spoligotyping confirmed the species identification of 19 M. tuberculosis and
13 M … None of these CAM 173 variant spoligotypes were found in the spolDB4 database and were …
All M. tuberculosis strains 175 from PTB patients that were spoligotyped had identical …
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K Faksri, F Drobniewski, V Nikolayevskyy… – Infection, Genetics and …, 2011 – Elsevier
… Eighty MTB Beijing isolates from CSF samples with IS6110 RFLPs (69 RFLP patterns were
available) and spoligotyping results (all 80 spoligotypes were available … Spoligotyping was
performed according to the manufacturer s instructions (Isogen Bioscience BV …
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[PDF] 骨结核临床分离菌株基因分型的特点
李文胜， 刘毅， 孙勇， 田苗， 刘忠泉… – 中华临床医师杂志 ( …, 2011 – clinicmed.net
… TM 琼脂糖购自赛百盛基因技 术有限公司。 DNA Ladder Marker 购自TaＫaRa 公司。
凝胶成像仪购自FOTO DＹNE 公司。 Spoligotyping 试 剂盒以及Miniblotter MN４５为荷兰
Isogen Biosｃienｃe 公司产品。 链霉亲和素为Sigam 公司产品。 化学发光 …
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MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS IN THE UNITED ARAB EMIRATES
A Saedi… – 2011 – qmro.qmul.ac.uk
… 130 3.2.11 Identification of the UAE isolates by spoligotyping….. 132 3.2.12 Comparison between
MIRU-typed and spoligotyped families in association with katG463/gyrA95 groupings ….. …
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[HTML] MYCOBACTERIUM BOVIS IN SWINE: SPOLIGOTYPING OF ISOLATES FROM ARGENTINA
S Barandiaran, EV Moras, A Adri& – Veterinary Medicine …, 2011 – hindawi.com
… Spoligotyping [14] was carried out by using the spoligotyping kit (Isogen Biosolutions BV, Ocimun …
Guerin (BCG) (ATCC 27289) were included as reference strains in each spoligotyping experiment …
isolates were defined as two or more M. bovis strains with identical spoligotypes. …
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[HTML] SPOLIGOTYPING OF CLINICAL MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM THE STATE OF MINAS GERAIS, BRAZIL
SS Miranda, WS Carvalho, PN Suffys… – Memórias do Instituto …, 2011 – SciELO Brasil
… was resuspended in the same TE buffer, stored at -20ºC and subjected to spoligotyping using
a previously published protocol (Kamerbeek et al. 1997) and a commercially available kit (Isogen
Life Science BV, Utrecht, The Netherlands). The hybridised spoligotypes in binary …
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EVALUATION OF SPOLIGOTYPING AND MIRU-VNTR FOR MYCOBACTERIUM BOVIS IN XINJIANG, CHINA
Z Sun, R Cao, M Tian, X Zhang, X Zhang… – Research in Veterinary …, 2011 – Elsevier
… Additionally, two new spoligotypes (SB1903 and SB1904) and special repeat numbers of 3 loci
(ETR … Spoligotyping was firstly analyzed and interpreted in octal codes (Dale et al., 2001) following
the instruction (http://www.mbovis.org) with a commercial kit (Isogen Bioscience BV …
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J Bazira, BB Asiimwe, ML Joloba… – BMC infectious …, 2011 – biomedcentral.com
… Spoligotyping. Standard spoligotyping was performed as previously described [29] using a
commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands). … Spoligotyping.
All the isolates had spoligotypes characteristic of M. tuberculosis. …
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I Cerezo, Y Jiménez, J Hernandez, T Zozio… – Infection, Genetics and …, 2011 – Elsevier
… Kamerbeek et al., 1997) according to the manufacturer s instructions (Isogen Bioscience, the …
Spoligotype International Type (SIT) and MIRU International Type (MIT) designate spoligotypes
and MIRU … was used to build minimum spanning trees based on spoligotyping and MIRU …
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[PDF] from digitaljournals.org
V Lisdawati, I Parwati… – Bulletin of Health …, 2011 – indonesia.digitaljournals.org
… PCR, denaturasi menggunakan thermocycler selama 10 menit pada suhu 99,9°C. Kemudian
pada mini blotter MN45 yang telah diletakkan membran spoligotyping kit (IM9702, Isogen
Biosolution, BV) berlabel 43 DNA spacer Mtb complex segera hibridisasikan DNA hasil PCR. …
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[PDF] INFECTION OF EURASIAN BADGERS (MELES MELES) WITH MYCOBACTERIUM BOVIS
A Balseiro, O Rodríguez, P González-Quirós… – prevalence, 2011 – uclm.es
… al., 2009). 125 MTC isolates were characterised hybridisation of biotin-labelled PCR
products 126 onto a spoligotyping membrane (Isogen Bioscience BV), followed by
spoligotyping 127 (Kamerbeek et al., 1997). Results were …
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HL Hsu, CC Lai, MC Yu, FL Yu, JC Lee… – European journal of …, 2011 – Springer
… commercially available spoligotyping kit (Isogen Bioscience BV, Maarssen, The Netherlands)
was used as described by the manufacturer. The fourth international spoligotyping database
(SpolDB4) database was used to assign genotype families to the spoligotypes obtained [14 …
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MOLECULAR TYPING OF MYCOBACTERIUM TUBERCULOSIS CIRCULATED IN MOSCOW, RUSSIAN FEDERATION
MV Afanas’ ev, LN Ikryannikova, EN Il’ina… – European Journal of …, 2011 – Springer
… spacers probes from the spoligotyping kit from Isogen Lifescience (cat. no. IM9701). 182 Eur
J Clin Microbiol Infect Dis (2011) 30:181–191 Page 3. Spoligotypes in binary format were entered
in an Excel spreadsheet and compared with the spoligotyping database SpolDB4 [13]. …
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ROLE OF 2-MONTH SPUTUM SMEARS IN PREDICTING CULTURE CONVERSION IN PULMONARY TUBERCULOSIS
WJ Su, JY Feng, YC Chiu… – European Respiratory …, 2011 – Eur Respiratory Soc
… All clinical isolates were genotyped by commercial spoligotyping kit (Isogen Bioscience BV,
Maarssen, the Netherlands). The “Beijing strain” was defined as deletion from spacer 1 to spacer
34 in the direct repeat region and the presence of (at least three of) spacers 35–43. …
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T JAGIELSKI, EWAA KOPEĆ… – Polish journal of …, 2011 – pjm.microbiology.pl
… Spoligotyping was perfor- med with a commercially available kit (Isogen Bio- science BV … e isolates
whose spoligotype patterns were already recorded in the database, were assigned “shared types”,
whereas those of spoligotypes identified for the first time, were …
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OY Limanskaya… – Cytology and Genetics, 2011 – Springer
… The PCR was carried out by a dry kit of reagents for DNA amplification (Isogen, Russian
Federation) in 20–50 μl of reaction mixture containing 4 mM NaCl, 50 mM KCl, 12 mM Tris HCl
(pH 8.0), 2.5 mM MgCl2, 200 μM dNTPs, 0.1–0.5 μM of each primer, and 1 unit of Taq DNA …
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STREPTOMYCIN RESISTANCE AND LINEAGE-SPECIFIC POLYMORPHISMS IN MYCOBACTERIUM TUBERCULOSIS GIDB GENE
FS Spies, AW Ribeiro, DF Ramos… – Journal of clinical …, 2011 – Am Soc Microbiol
… Genotyping.Spoligotyping was performed using a commercial kit (Isogen Biosciences BV,
Netherlands … it was classified as LAM3 and S/convergent sublineage by spoligotyping but as non …
Spoligotypes evolve through successive loss of spacer DNA sequences (16), and since …
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YL Guo, Y Liu, SM Wang, CY Li… – … of Tuberculosis and …, 2011 – ingentaconnect.com
… Of the (Isogen Bioscience BV, De Meern, The Netherlands). … 1–1800 = shared spoligotypes. Table
3 Results of MIRU typing and comparison of the three typing methods Isolate MIRU locus number
IS6110-RFLP Cluster 2 4 10 16 20 23 24 26 27 31 39 40 Spoligotyping MIRU …
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SF Huang, WJ Su, HY Dou, JY Feng… – Infection, Genetics and …, 2011 – Elsevier
… Spoligotyping was performed according to the method reported by Kamerbeek et al. (Kamerbeek
et al., 1997) using a commercial kit (Isogen Biosolutions, Netherlands). The spoligotypes were
identified according to the database SpoDB4.0 (Brudey et al., 2006), and the SIT …
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THE GENOTYPE CHARACTERISTIC OF CLINICAL ISOLATED STRAINS GETTING FROM BONE TUBERCULOSIS
LI Wen-sheng, LIU Yi, SUN Yong… – Chinese Journal of …, 2011 – global.clinicmed.net
… TM 琼脂糖购自赛百盛基因技 术有限公司。 DNA Ladder Marker 购自 TaＫaRa 公司。
凝胶成像仪购自 FOTO DＹNE 公司。 Spoligotyping 试 剂盒以及 Miniblotter MN４５为荷兰
Isogen Biosｃienｃe 公司产品。 链霉亲和素为 Sigam 公司产品。 化学发光 …
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MULTIPLE STRAINS OF< I> MYCOBACTERIUM BOVIS</I> REVEALED BY MOLECULAR TYPING IN A HERD OF CATTLE
EES Figueiredo, DF Ramos, L Medeiros… – The Veterinary …, 2011 – Elsevier
… to the spoligo-membrane was performed according to the manufacturer’s instructions (Isogen
Life Sciences). … The strains clustered by spoligotyping were analysed by VNTR-PCR to confirm
clonal relationships … Four spoligotypes were identified among the 12 M. bovis isolates. …
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FS Spies – 2011 – lume.ufrgs.br
… restriction fragment lenght polymorphism) da sequência de inserção IS6110, spoligotyping e
unidade de repetição de micobactéria- repetição em tandem de número variável (MIRU- …
Spoligotyping é uma técnica de genotipagem que explora a variabilidade da região …
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Y Hu, X Ma, EA Graviss, W Wang… – Epidemiology and …, 2011 – Cambridge Univ Press
… Spoligotyping was performed by using a com- mercial kit from Isogen Bioscience BV (Maarssen …
0.04), and resistance to RIF and SM (P=0.04) (Table 1). Spoligotyping identified 243 … Thirty-three
(9.4%) isolates had unique/novel spoligotypes, of which similarity analysis found that …
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[HTML] MODERN AND ANCESTRAL GENOTYPES OF MYCOBACTERIUM TUBERCULOSIS FROM ANDHRA PRADESH, INDIA
SK Thomas, CC Iravatham, BH Moni, A Kumar… – PloS one, 2011 – dx.plos.org
… the help of commercially available line probe arrays (Isogen Biosciences BV … tuberculosis complex
genetic diversity: mining the fourth international spoligotyping database (SpolDB4 … KP (2006)
Identifying Mycobacterium tuberculosis complex strain families using spoligotypes. …
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GSS Eldin, I Fadl-Elmula, MS Ali… – BMC infectious …, 2011 – biomedcentral.com
… 21] using a commercial kit to test for 43 individual spacers (Isogen, IJsselstein, The … Spoligotyping
provided a relatively simple and rapid means of describing the population of … improved
discrimination of strains sharing the CAS1_Dehli and Beijing spoligotypes when using MIRU …
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TM Hlokwe, AO Jenkins, EM Streicher… – … Journal of Veterinary …, 2011 – scielo.org.za
… One of the observed spoligotypes (SB0130) was dominant and represented 75% of isolates …
Spoligotyping Spoligotyping was done according to a standardised international method
(Kamerbeek et al. 1997) using a commercially available kit (Isogen Biosciences BV, Maarsen). …
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GENOTYPING DID NOT EVIDENCE ANY CONTRIBUTION OF MYCOBACTERIUM BOVIS TO HUMAN TUBERCULOSIS IN BRAZIL
A Rocha, AR Elias, LF Sobral, DF Soares… – Tuberculosis, 2011 – Elsevier
… using PCR and hybridization conditions on a commercially available membrane (Isogen
Bioscience BV … All isolates that yielded spoligotypes that were recognized as not previously
described patterns were submitted to at least one additional spoligotyping assay and …
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CK Mlambo – 2011 – wiredspace.wits.ac.za
… 61 Table 3.6 Description of the orphan MIRU patterns (n=45) from this study and
corresponding spoligotyping-based information (octal code, SIT, and clade)…………
64 Table 3.7 MIRU profiles of the four newly created spoligotypes (10 …
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USE OF SPOLIGOTYPE ANALYSIS TO DETECT LABORATORY CROSS-CONTAMINATION
B Nivin, J Driscoll, T Glaser, P Bifani… – Infection control and …, 2000 – JSTOR
… The spoligotyping analysis was performed using a commercially available kit (Isogen Bioscience
BV … In several cases of identical spoligotypes, the Wadsworth Center indicated that the …
Spoligotyping is an effective, relatively inexpensive (approximately 10% the cost of RFLP …
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CLINICAL AND GENOTYPIC CHARACTERISTICS OF CHILDHOOD TUBERCULOSIS IN CHONGQING, CHINA
L Xing, R Liu, Q Li, Z Peng… – European Journal of Clinical …, 2011 – Springer
… Of 105 isolates spoligotyped, the major spoligotypes found were … Spoligotyping was performed
according to the internation- ally standardized protocol [5]. A commercially available spoligotyping
kit (Isogen Bioscience BV, Maarssen, The Netherlands) was used as described …
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TUBERCULOSIS DUE TO MYCOBACTERIUM BOVIS AND MYCOBACTERIUM CAPRAE IN SHEEP
M Muņoz Mendoza, L Juan, S Menéndez… – The Veterinary …, 2011 – Elsevier
… by hybridisation of the biotin-labelled PCR product onto spoligotyping membrane (Isogen
Bioscience BV). … Isolates were characterised by spoligotyping as M. caprae SB0157 (flock A) and
M. bovis … close contact with cattle or goats infected by the same spoligotypes (Table 1 …
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[HTML] MYCOBACTERIUM BOVIS INFECTION IN GOATS FROM THE NORTHEAST REGION OF BRAZIL
SSS Higino, SR Pinheiro, GO Souza… – Brazilian Journal of …, 2011 – SciELO Brasil
… Isolate was characterised by spoligotyping as described (7). The amplified product
was detected by hybridization of the biotin-labelled PCR product onto spoligotyping
membrane (Isogen Bioscience BV, Maarssen, The Netherlands). …
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JUNREN SUN, HYUN DOU, SYI LEE… – Apmis, 2011 – Wiley Online Library
… on isolates from areas where the Beijing strain is prevalent as most isolates in this family share
the same spoligotypes (1, 5 … Spoligotyping … to the manufacturers’ protocol, to a set of 43 oligonucle-
otides of DR spacers on a membrane using the Isogen Bioscience spoligotyping kit …
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2010
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L Flores, LG Jarlsberg, EY Kim… – Journal of clinical …, 2010 – Am Soc Microbiol
… 2004 was performed with standardized methods (15) using a membrane with 43 oligonucleotides
representing the different direct variable repeats (DVRs) immobilized in specific locations (Ocimum
Biosolutions, Inc., Gaithersburg, MD). Beginning in 2004, spoligotyping of the …
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A Von Groll, A Martin, C Felix… – FEMS Immunology …, 2010 – Wiley Online Library
… RD Rio BrgR: 5′-CACCGCCACGCTGAATGAGACCA-3′, –. Genotyping by
spoligotyping. Spoligotyping was performed with a commercial kit (Ocimum Biosolutions
BV, India) according to standard procedures (Kamerbeek et al., 1997). …
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[HTML] Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype
A Von Groll, A Martin, M Stehr, M Singh, F Portaels… – PloS one, 2010 – dx.plos.org
… Strains were fully characterized and analyzed for the presence of mutations conferring resistance
to RMP and INH. Spoligotyping was performed with a commercial kit (Ocimum Biosolutions BV,
India) according to standard procedures [29]. Molecular characterization. …
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SO Viegas, A Machado, R Groenheit… – BMC …, 2010 – biomedcentral.com
… using a commercially available kit (Isogen Life Science BV, Utrecht, The Netherlands).
Spoligotyping results were analysed with the BioNumerics Software ver. 5.01 (Applied Maths,
Kortrijk, Belgium). Database comparison and geographical distribution of spoligotypes. …
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[PDF] from academicjournals.org
J Zhang, X Wu, Y Yang, Y Zhuang… – African Journal of …, 2010 – academicjournals.org
… The amplified PCR products were hybridized with spoligotyping Biodyne C membrane immobilized
43 oligonucleotide probes (Isogen Life Science, Holland). … A total of 8 distinct spoligotyping patterns
were observed. … 7 isolates had unique spoligotypes (Figure 1, Tables 1 and 2 …
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PC Chuang, YMA Chen, HY Chen… – Infection, Genetics and …, 2010 – Elsevier
… 2.2. Genotype analysis. Spoligotyping using a commercially available kit (Isogen Bioscience
BV, Maarssen, The Netherlands … Spoligotypes and RFLP patterns were scanned and analyzed
with Bionumerics ® software (version 4.601; Applied Maths, Kortrijk, Belgium). …
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[HTML] ASSOCIATIONS BETWEEN MYCOBACTERIUM TUBERCULOSIS STRAINS AND PHENOTYPES
T Brown, V Nikolayevskyy, P Velji… – Emerging Infectious …, 2010 – ncbi.nlm.nih.gov
… Spoligotyping was performed according to the manufacturer’s instructions (Isogen Lifescience,
IJsselstein, the Netherlands) (9). Images were digitized and entered into BioNumerics software
by using the BNIMA module (Applied Maths). Spoligotypes were assigned to families …
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F Milián Suazo, B Harris, C Arriaga Díaz… – Revista mexicana de …, 2010 – scielo.org.mx
… Spoligotyping se realizó tal como lo describe Kamerbeek et al (17) . De manera
resumida, la amplificación del … por 30 seg. La hibridización se realizó de acuerdo
a las recomendaciones del fabricante (Isogen). El ADN hibridado …
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EN Noguti, CQF Leite, AC Malaspina… – Memórias do Instituto …, 2010 – SciELO Brasil
… Spoligotyping – Spoligotyping was performed in all 93 M. tuberculosis isolates, using the standard
method … of 43 spacer oligonucleotides covalently linked to the spoligo-membrane (Isogen Life
Sciences … Spoligotypes were reported using an octal code in which the 43-digit binary …
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Y Hu, S Hoffner, W Jiang… – BMC infectious …, 2010 – biomedcentral.com
… Spoligotyping was carried out by using the commercial kit from Isogen Bioscience BV … Table 3.
Spoligotyping pattern and frequency of the MTB isolates included. … B, Kurepina N, Bennett KP:
Identifying Mycobacterium tuberculosis complex strain families using spoligotypes. …
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[HTML] MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM BOVIS ISOLATES FROM ETHIOPIAN CATTLE
D Biffa, E Skjerve, J Oloya, A Bogale… – BMC veterinary …, 2010 – biomedcentral.com
… spacers were then hybridized with the known oligonucleotide spacers ligated to the membrane
(Isogen). … The discriminatory power (HGDI) of the spoligotyping method was calculated to be 0.81.
Also on the basis of the above findings (the number of distinct spoligotypes (S) = 12 …
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[HTML] MYCOBACTERIUM TUBERCULOSIS BEIJING STRAIN, BAMAKO, MALI
B Diarra, S Siddiqui, D Sogoba… – Emerging infectious …, 2010 – ncbi.nlm.nih.gov
… See the article”Global Distribution of Mycobacterium tuberculosis Spoligotypes” in volume 8 on
page 1347. … Spoligotyping using a commercially available kit (Spoligotyping Isogen Life Science,
De Meern, the Netherlands) showed characteristics of the Beijing clade (Appendix …
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EVALUATION OF 24 LOCUS MIRU-VNTR GENOTYPING OF MYCOBACTERIUM TUBERCULOSIS ISOLATES IN CANADA
S Christianson, J Wolfe, P Orr, J Karlowsky… – Tuberculosis, 2010 – Elsevier
… some cases. 22 Blots were purchased from Isogen Biosolutions and run according
to the manufacturer’s directions. Spoligotyping patterns were converted to the “octal”
pattern using the previously described formula. 23 Data …
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C McGoldrick, C Coghlin, A Seagar… – BMJ Case …, 2010 – casereports.bmj.com
… At SMRL spoligotyping was performed using a commercial assay available from Isogen Life
Science (3600 BT; Maarssen, The Netherlands) and 15-locus Mycobacterial Interspersed
Repetitive Unit-Variable Number Tandem Repeat (MIRU-VNTR) typing, performed using the …
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MOLECULAR EPIDEMIOLOGY OF HUMAN CASES OF TUBERCULOSIS BY MYCOBACTERIUM BOVIS IN MEXICO
F Milián-Suazo, L Pérez-Guerrero… – Preventive veterinary …, 2010 – Elsevier
… Coding allows easy comparisons of spoligotypes from different laboratories and from different
parts of … Spoligotyping was performed following a standard protocol (Kemerbeek et al., 1997). … 6
spacer sequences from M. bovis BCG were covalently bound (ISOGEN, Maarssen, the …
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MA Merza, P Farnia, AM Salih, MR Masjedi… – …, 2010 – content.karger.com
… [19] , with a commercially available kit, according to the manufacturer’s instructions (Isogen Biosci-
ence, The … Analysis of Spoligotyping Data A total of 133 distinct spoligopatterns were identi- fied …
The distribution of spoligotypes (T, CAS, EAI, Haarlem, X, Beijing, Or- phan and …
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[HTML] CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS BEIJING ISOLATES FROM THE MEDITERRANEAN AREA
M Alonso, NA Rodriguez, C Garzelli… – BMC …, 2010 – biomedcentral.com
… deletion of the spacers 1-34 and the characteristic hybridization pattern of spacers 35-43, and
the remaining seven corresponded to variant “Beijing-like” spoligotypes. … Spoligotyping was
performed following the manufacturer’s instructions (Isogen, Netherlands). …
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T Jagielski, E Augustynowicz-Kopeć… – Journal of clinical …, 2010 – Am Soc Microbiol
… CTAB) method (47) were stored at −20°C before being subjected to spoligotyping using a …
hybridized to a set of 43 oligonucleotides covalently bound to a membrane (Isogen Life Science
BV … Spoligotypes in binary format were converted to an octal code for comparison with the …
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R Lopez-Alvarez, C Badillo-Lopez… – BMC …, 2010 – biomedcentral.com
… To determine lineage, the 57 strains (48 MTb and 9 M. bovis) from the MTC were spoligotyped
and binary outcomes were compared with the shared type (ST) number and lineages and
sublineages reported by … Spoligotyping. … Spoligotypes were reported using an octal code [74]. …
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CC Lai, CK Tan, SH Lin, CH Liao… – Chest, 2010 – chestjournal.chestpubs.org
… Spoligotyping was performed according to the internationally standardized protocol to identify
and differentiate … 10 A commercially available kit (Isogen Bioscience BV; Maarssen, The
Netherlands) was … database was used to assign genotype families to the spoligotypes obtained …
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K Mallard, R McNerney, AC Crampin… – Journal of clinical …, 2010 – Am Soc Microbiol
… on these isolates with the aid of a commercially available kit (Isogen Biosciences BV … These isolates
were also spoligotyped and had previously been characterized by IS6110-RFLP … Spoligotyping
versus Beijing and LAM PCR results for 377 clinical M. tuberculosis isolates used …
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[HTML] CLUSTERING OF MYCOBACTERIUM TUBERCULOSIS CASES IN ACAPULCO: SPOLIGOTYPING AND RISK FACTORS
E Nava-Aguilera, E Harris, S Mitchell… – Clinical and …, 2010 – hindawi.com
… DNA with 43 oligonucleotides covalently linked to a nylon membrane (Isogen Bioscience BV …
SpolTools permitted the analysis of M. tuberculosis spoligotyping data to identify emerging … and
visualization of the probable evolutionary relationships between the spoligotypes in our …
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TC Rodwell, AJ Kapasi, M Moore… – International Journal of …, 2010 – Elsevier
… For this study, we also examined all available spoligotypes in the US national surveillance
database of M. bovis isolates sent to the National Veterinary Services Laboratories … Spoligotyping
was performed by all laboratories using the Spoligo Kit (Isogen Bioscience BV …
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S Rodríguez, B Romero, J Bezos, L De Juan… – Veterinary …, 2010 – Elsevier
… The isolates were spoligotyped following the protocol described by Kamerbeek et al … Frequencies
of the 43 spacers of the DR locus that are included in the spoligotyping membrane (designed …
Data expressed as percentage of spoligotypes (n = 252) in which the spacer is present …
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I Parwati, B Alisjahbana, L Apriani… – Journal of Infectious …, 2010 – jid.oxfordjournals.org
… of the Beijing genotype per se or to confounding by drug resistance, which has been linked to
the Beijing genotype in different settings [1]. We previously spoligotyped a large … Spoligotyping
was performed using a commercial kit (Isogen Bioscience) according to the …
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CA Molina-Torres, J Castro-Garza… – Journal of medical …, 2010 – Soc General Microbiol
… (1997) was used. PCR amplification of the direct repeat locus was performed on heat-treated
cell suspensions. Biotin-labelled PCR products were detected by hybridization onto a
spoligotyping membrane (Isogen Bioscience). Microarray analysis. …
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[HTML] DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS GENOTYPES CIRCULATING IN NDOLA, ZAMBIA
C Mulenga, I Shamputa, D Mwakazanga… – BMC infectious …, 2010 – biomedcentral.com
… Spoligotyping was performed using a commercial kit (Isogen Bioscience BV, Maarssen, The
Netherlands) according … Spoligotyping and MIRU-VNTR patterns were compared to the
international Spol DB4.0 … Spoligotypes that were not present in the Spol DB4.0 are referred to …
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PHENOTYPIC AND GENOTYPIC ANALYSIS OF MULTIDRUG-RESISTANT TUBERCULOSIS IN ETHIOPIA
M Agonafir, E Lemma… – … of Tuberculosis and …, 2010 – ingentaconnect.com
… to the extraction protocol described by van Soolingen et al.14 Spoligotyping was performed … the
commercially available kit from Isogen Bioscience BV, Maarssen, The Netherlands, according
to … Spoligotypes described only once (non-clustered) in this study and in the SpolDB4 …
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FM Suazoa, B Harrisb, CA Díazc… – Revista Mexicana de …, 2010 – scielo.org.mx
… Spoligotyping was performed essentially as described by Kamerbeek(17). Briefly,
amplification of DNA was run in … for 30 sec. Hybridization was performed as
recommended by the manufacturer (Isogen). Hybridized DNA was detected …
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CC Lai, CK Tan, SH Lin, CH Liao… – European journal of …, 2010 – Springer
… by serial dilution on agar plates as described previously [1]. Spoligotyping, according to … the M.
tuberculosis complex isolates [3]. A commercially available kit (Isogen Bioscience BV … 1
Dendrograms of spoligotypes generated by clustered Myco- bacterium tuberculosis strains after …
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OY Limanskaya, TN Mukhina, VN Stepanshina… – Molecular Biology, 2010 – Springer
… carrying AGC AGA; HB125, carrying AGC ACC, and H37Rv with the wild type codon
315 (negative control for amplification). The reaction mixture (20–50 μl) was prepared
from a dry amplification kit (Isogen, Russia). Its com …
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A Al-Maniri, JPN Singh, O Al-Rawas… – … of Tuberculosis and …, 2010 – ingentaconnect.com
… Figure Spoligotyped distribution of … Molecular typing of Mycobacterium tuberculosis isolates
from a rural area of Kanpur by spoligotyping and mycobacterial interspersed re- petitive units
(MIRUs) typing. … Predominant tubercu- losis spoligotypes, Delhi, India. …
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J Zhang, E Abadia, G Refregier… – Journal of medical …, 2010 – Soc General Microbiol
… Thus, as spoligotypes are considered both as phylogenetic and epidemiological markers (Asiimwe
et al., 2008; Sola et al., 2001; Tanaka & Francis, 2006 … Both sets had been previously genotyped
by spoligotyping using commercial membranes (Isogen Biosciences). …
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S Rosales, L Pineda-García… – BMC …, 2010 – biomedcentral.com
… All isolates were spoligotyped in order to identify the predominant genotypes within this … SG
performed and analyzed RFLP; carried out bioinformatics analysis of spoligotyping and RFLP
results … NR performed database analysis of the spoligotypes and helped draft the manuscript …
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JS Sutherland, BC de Jong, DJ Jeffries, IM Adetifa… – PloS one, 2010 – dx.plos.org
… 10ng of DNA was used for spoligotype analysis with commercially available membranes (Isogen
Biosciences, The Netherlands). … Contributed reagents/materials/analysis tools: BCdJ. Wrote the
paper: JSS MOCO. Performed spoligotyping: BCdJ. Statistical analysis: DJJ. …
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[BOOK] RAPID TESTS FOR MULTIDRUG RESISTANT TUBERCULOSIS IN LOW INCOME SETTINGS
F Bwanga – 2010 – mbl.mak.ac.ug
… In paper V, we applied spoligotyping to study the clustering rate and predominant genotypic strains
of 99 MDR-TB strains isolated from patients in Kampala. Eighty three percent of the strains
occurred in clusters, and the T2 lineage was the largest single cluster. Conclusion. …
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2009
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Spoligotyping for molecular epidemiology of the Mycobacterium tuberculosis complex
JR Driscoll – Methods Mol. Biol, 2009 – Springer
… 1. Spoligotyping membrane (Ocimum Biosolutions Inc., for- merly Isogen Biosciences BV … potential
of additional spacer sequences for “expanded” or “extended” spoligotyping (25, 26). … spacer
sequences would improve the differentiability of the commonly observed spoligotypes. …
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PJ Freidlin, D Goldblatt… – Journal of clinical …, 2009 – Am Soc Microbiol
… The fast DNA extraction method (amount used, 2 μl) was also successfully employed for
spoligotyping (Ocimum Biosolution Company, India). … In addition, further CAS MIRU-VNTR types
and associated spoligotypes were identified in the literature (3, 6, 7, 58, 61, 62). …
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MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS IN THE UNITED ARAB EMIRATES (UAE)
A Saaidi… – 2009 – qmro.qmul.ac.uk
… HYPERLINK \l “_Toc237239861” **3.2.11 Identification of the UAE isolates by spoligotyping *
PAGEREF _Toc237239861 \h **133**. * HYPERLINK \l “_Toc237239862” **3.2.12 Comparison
between MIRU-typed and spoligotyped families in association with katG463/gyrA95 …
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DD An, NTH Duyen, NTN Lan, DTM Ha… – Antimicrobial agents …, 2009 – Am Soc Microbiol
… with primers Dra (biotin labeled) and Drb, hybridized to the spoligotype membrane (Isogen
Bioscience BV … were assigned to the Euro-American clade on the basis of spoligotyping patterns …
LSP types and spoligotypes of 109 consecutive FQ-resistant isolates and 109 consecutive …
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[PDF] NOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSIS
TB Nosocomial – Indian J Tuberc, 2009 – lrsitbrd.nic.in
Page 1. Indian Journal of Tuberculosis [Indian J Tuberc 2009; 56:59-61] Editorial
NOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSIS
Indian Journal of Tuberculosis Vol. 56 New Delhi, April 2009 No. 2 …
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[HTML] SUPERVISED LEARNING FOR THE AUTOMATED TRANSCRIPTION OF SPACER CLASSIFICATION FROM SPOLIGOTYPE FILMS
D Jeffries, N Abernethy… – BMC bioinformatics, 2009 – biomedcentral.com
… a standardized method [4] using commercially prepared membranes from Isogen Biosciences
(Maarsen … Crawford JT: Transfer of a Mycobacterium tuberculosis genotyping method,
Spoligotyping, from a … AR, Tanaka MM: spolTools: online utilities for analyzing spoligotypes of …
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PJ Freidlin, D Goldblatt… – Journal of clinical …, 2009 – Am Soc Microbiol
… In addition, further CAS MIRU-VNTR types and associated spoligotypes were identified in …
Spoligotyping.Spacer oligonucleotide typing (spoligotyping) for 43 spacers of the direct repeat
region (9 … performed using a kit according to the manufacturer’s instructions (Isogen/Omicium …
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X Jiang, C Lu, F Gao, F Wang, W Zhang… – Microbes and …, 2009 – Elsevier
… Spoligotyping was performed as previously described [16 … in 2 × SSPE (1 × SSPE is 0.18 M NaCl,
10 mM NaH 2 PO 4 , and 1 mM EDTA) −0.5% sodium dodecyl sulfate (SDS) at 60 °C and then
incubated in 1:4000 diluted streptavidin peroxidase conjugate (Isogen Bioscience) for …
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JR Sun, SY Lee, HY Dou… – European Journal of Clinical …, 2009 – Springer
… Page 2. in the Isogen Bioscience spoligotyping kit (Isogen Life Science, the Netherlands). … Among
the 323 clinical MTB isolates, a total of 78 different spoligotypes were found. One hundred and
sixty-nine isolates were determined to belong to the Beijing family. …
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[HTML] Mycobacterium bovis en Argentina: aislamientos de gatos tipificados por spoligotyping
MJ Zumárraga, M Martínez Vivot… – Revista argentina de …, 2009 – SciELO Argentina
… (8) , and using the spoligotyping kit (Isogen Biosolutions BV … Each of the different spoligotypes
were allocated a number. … H37Rv (ATCC 27294) and M. bovis Bacillus Calmette-Guerin (BCG)
(ATCC 27289) were included as reference strains in each spoligotyping experiment. …
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Spoligotyping for molecular epidemiology of the Mycobacterium tuberculosis complex
JR Driscoll – Methods Mol. Biol, 2009 – Springer
… 1. Spoligotyping membrane (Ocimum Biosolutions Inc., for- merly Isogen Biosciences BV … potential
of additional spacer sequences for “expanded” or “extended” spoligotyping (25, 26). … spacer
sequences would improve the differentiability of the commonly observed spoligotypes. …
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BC De Jong, M Antonio, T Awine… – Journal of clinical …, 2009 – Am Soc Microbiol
… After spectrophotometry, we used 10 ng of DNA for spoligotype analysis (16) with commercially
available membranes (Isogen Biosciences, Maarssen, The Netherlands … If spoligotype analysis
did not result in a clear pattern of spacers, the isolate underwent repeat spoligotyping. …
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H Valvatne, H Syre, M Kross… – Journal of …, 2009 – Br Soc Antimicrob Chemo
… identification test (GenProbe Incorporated, San Diego, CA, USA) and by spoligotyping (Isogen
Bioscience BV … A total of 43 different spoligotypes were observed among the 96 isoniazid-resistant …
of the 63 isolates that could not be differentiated by spoligotyping produced diverse …
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S Tafaj, J Zhang, Y Hauck, C Pourcel… – Journal of clinical …, 2009 – Am Soc Microbiol
… ethambutol (2.0 μg/ml) (5). Identification at the species level was performed by phenotypic methods
and verified by a genotypic method on isolates with rare spoligotypes and/or … Spoligotyping was
performed using a commercial membrane (Isogen, The Netherlands …
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PICPI Cafrune, LGPLG Possuelo… – Canadian Journal …, 2009 – NRC Research Press
… was performed using a commercial kit according to the manufacturer’s instructions (Isogen
Biosciences BV … Table 1. Accuracy of spoligotyping results according to culture grading. … A total
of 44 different spoligotypes were identified; 112 isolates (83%) were included in 21 shared …
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MB Boniotti, M Goria, D Loda… – Journal of clinical …, 2009 – Am Soc Microbiol
… repeat locus were detected by hybridization onto a spoligotyping membrane (Isogen Bioscience
BV … into 64 different patterns, while the seven M. caprae spoligotypes were subdivided … The
discrimination provided by combination of spoligotyping and ETR methods allowed for a …
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HY Dou, JJ Lu, CW Lin, JR Chang, JR Sun… – Journal of microbiological …, 2009 – Elsevier
… Spoligotyping was carried out using a spoligotyping kit according to the manufacturer’s instructions
(Isogen Bioscience BV). Spoligotypes common to more than one strain were designated as shared
types (ST) and assigned a shared international type number (SIT) according to …
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[PDF] Comparison of phenotypic and genotypic methods for pyrazinamide susceptibility testing
S Shenai, C Rodrigues, M Sadani, N Sukhadia… – Indian J Tuberc, 2009 – medind.nic.in
… All the PZA resistant isolates were further spoligotyped to identify M. bovis, if present. … Finger
printing – Spoligotyping All the 63 culture isolates were subjected to spoligotyping using a
commercially available Isogen kit, Netherlands as described by Kamerbeek et al.11 Briefly …
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J Yorsangsukkamol, A Chaiprasert, T Prammananan… – Tuberculosis, 2009 – Elsevier
… 2.4. Spoligotyping. Spoligotyping was performed with a commercially available
kit (Isogen Bioscience BV, Maarsen, The Netherlands). … 25 All obtained spoligotypes
were compared to SpolDB4 for analysis. 2.5. Data analysis. …
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S Shakoor, M Tanveer, Y Rafiq… – Scandinavian …, 2009 – informahealthcare.com
… Five different resistance phenotypes were identified among the isolates spoligotyped: isolated
low-level isoniazid resistance (resistant to 0.2 µg/ml) in 1 isolate; high-level isoniazid resistance
(resistant to 1 … Spoligotypes Spoligotyping grouped 66 isolates into 9 different clusters. …
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BA van der Meer… – Factors Underlying the Success … – repository.ubn.kun.nl
… min. Spoligotyping was performed using a commercial kit (Isogen Bioscience,
Maarssen, The Netherlands). The … min. Spoligotyping was performed using a
commercial kit (Isogen Bioscience, Maarssen, The Netherlands). The …
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Emergence of new forms of totally drug-resistant tuberculosis bacilli
AA Velayati, MR Masjedi, P Farnia… – Chest, 2009 – chestjournal.chestpubs.org
… by polymerase chain reaction (PCR) using primers derived from the direct repeat sequence
(Isogen Bioscience; Maarssen, the … and CAS (n = 4; 17.5%) [Table 2]. The VNTR profiles of
spoligotypes strains were … Spoligotyping Patterns and VNTR Profiles of XDR and TDR Patients. …
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I Parwati, R van Crevel, J de Beer… – Factors Underlying the … – repository.ubn.kun.nl
… of rpoB-hotspot region Three hundred and twenty five spoligotyped isolates were … Mycobacterium
tuberculosis complex genetic diversity: mining the fourth international spoligotyping database
(SpolDB4 … mutations in katG, rpoB, rpsL and rrs genes with spoligotypes of multidrug …
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R Van Crevel, I Parwati… – Journal of Infectious …, 2009 – jid.oxfordjournals.org
… Mycobacterial DNA was extracted by bringing 2 loops of bacterial mass from a M.
tuberculosis culture in saline and subsequently heating it at 95°C for 5 min.
Spoligotyping was performed using a commercial kit (Isogen Bioscience). …
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ZH Helal, MSED Ashour, SA Eissa… – Journal of clinical …, 2009 – Am Soc Microbiol
… was amplified with primers DRa and DRb, and the amplified biotinylated products were hybridized
to a set of 43 oligonucleotides covalently bound to a membrane (Isogen Life Science BV …
Comparison of spoligotyping results with database types.Spoligotypes in binary …
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AA Velayati, P Farnia, TA Ibrahim… – …, 2009 – content.karger.com
… For spoligotyping, the direct repeat region was am- plified by PCR using primers derived from
the direct repeat se- quence (Isogen Bioscience). … Spoligopatterns and VNTR Profiles When the
spoligotype patterns were compared with earlier published spoligotypes [5] , our …
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Mycobacterium tuberculosis genotypic diversity in pyrazinamide-resistant isolates of Iran
F Doustdar, AD Khosravi… – Microbial Drug …, 2009 – online.liebertpub.com
… Spoligotyping was performed with a commercial kit (Isogen Bioscience BV, Maarsen, The … Only
1 (5.0%) of the 20 PZA-monoresistant isolates had spoligotyping patterns characteristic … Therefore,
the CAS superfamily spoligotypes showed no important correlation with MDR-TB as …
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Epidemiology of Mycobacterium bovis infection in wild boar (Sus scrofa) from Portugal
N Santos, M Correia-Neves… – Journal of wildlife …, 2009 – Wildlife Dis Assoc
… signatures were compared with those available in SpolDB4.0, the international spoligotyping
database (Brudey et al … The spoligotypes of 14 M. bovis isolates obtained from study areas B and
G … originated from study area B. Isolates from the study area C were not spoligotyped. …
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PI Cafrune – 2009 – lume.ufrgs.br
… Spoligotyping technique presented good results when applied directly to clinical samples, once
89% of samples presented agreeing results. Among 135 patients isolates genotyped, it was found
44 differnt spoligotypes, 112 (83%) were shared types and 23(17%) were unique. …
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BB Asiimwe, J Asiimwe, G Kallenius… – Veterinary …, 2009 – veterinaryrecord.bmj.com
… The isolates were spoligotyped using the DNA extracted at the identification stage. … The
spoligotypes were analysed by BioNumerics software, version 5.0 (Applied Maths) as character …
were compared with those in SpolDB4.0, the international spoligotyping database (Brudey …
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High resolution typing system for pathogenic Mycobacterium tuberculosum
PS Keim, RS Spurgiesz… – US Patent 7,592,135, 2009 – Google Patents
… Spoligotyping is currently the most widely used PCR- based method of M. tuberculosum
genotyping due to the 65 relative technical ease of … In a database of 3,319 spoligotypes from
isolates collected in geographically distinct areas, seven spoligotype patterns were found for …
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R Cicero, H Olivera, A Hernández-Solis… – European journal of …, 2009 – Springer
… a commercially available kit in accordance with the manufacturer’s recommendations (Isogen
Bioscience BV … and consistent identification of M. bovis was obtained by spoligotyping, showing
a … cattle, where even isolates from the same herd show different spoligotypes [26] and …
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R Stavrum, VP Myneedu, VK Arora, N Ahmed… – PloS one, 2009 – dx.plos.org
… C membranes with the 43 synthetic oligonucleotides covalently bound to the membranes (Isogen
Bioscience BV … (2004) Predominant tuberculosis spoligotypes, Delhi, India. … repetitive-
unit-variable-number tandem-repeat typing system combined with spoligotyping for population …
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Predominance of Mycobacterium tuberculosis EAI and Beijing lineages in Yangon, Myanmar
S Phyu, R Stavrum, T Lwin… – Journal of clinical …, 2009 – Am Soc Microbiol
… (35). Spoligotyping was performed with commercially available activated Biodyne C membranes
that had 43 synthetic oligonucleotides covalently bound to the membranes (Isogen Bioscience
BV … Prevalence of spoligotypes in Yangon.The spoligotyping results obtained …
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[HTML] Tokyo-172 BCG vaccination complications, Taiwan
R Jou, WL Huang… – Emerging Infectious Diseases, 2009 – ncbi.nlm.nih.gov
… BCG, DNA samples were initially screened using a GenoType kit (Hain Lifescience GmbH, Nehren,
Germany), multiplex PCR (7), and pncA sequencing (8). In addition, spoligotyping was performed
with a commercial kit (Isogen Bioscience BV, Maarssen, the Netherlands). …
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Immunologic analysis of HIV-uninfected taiwanese children with BCG-induced disease
WI Lee, FC Liang, JL Huang, TH Jaing… – Journal of clinical …, 2009 – Springer
… also used to identify and differentiate M. tuberculosis complex isolates in this study [38], using a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands). The resolved spoligotypes were M. tuberculosis complex strain specific. The spoligotyping profile of …
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X Jiang, C Lu, F Gao, F Wang, W Zhang… – Microbes and …, 2009 – Elsevier
… Spoligotyping was performed as previously described [16 … in 2 × SSPE (1 × SSPE is 0.18 M NaCl,
10 mM NaH 2 PO 4 , and 1 mM EDTA) −0.5% sodium dodecyl sulfate (SDS) at 60 °C and then
incubated in 1:4000 diluted streptavidin peroxidase conjugate (Isogen Bioscience) for …
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[PDF] NOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSIS
TB Nosocomial – Indian J Tuberc, 2009 – lrsitbrd.nic.in
Page 1. Indian Journal of Tuberculosis [Indian J Tuberc 2009; 56:59-61] Editorial
NOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSIS
Indian Journal of Tuberculosis Vol. 56 New Delhi, April 2009 No. 2 …
Related articles – View as HTML –
DD An, NTH Duyen, NTN Lan, DTM Ha… – Antimicrobial agents …, 2009 – Am Soc Microbiol
… with primers Dra (biotin labeled) and Drb, hybridized to the spoligotype membrane (Isogen
Bioscience BV … were assigned to the Euro-American clade on the basis of spoligotyping patterns …
LSP types and spoligotypes of 109 consecutive FQ-resistant isolates and 109 consecutive …
Cited by 23 – Related articles – All 9 versions – Import into BibTeX
PJ Freidlin, D Goldblatt… – Journal of clinical …, 2009 – Am Soc Microbiol
… In addition, further CAS MIRU-VNTR types and associated spoligotypes were identified in …
Spoligotyping.Spacer oligonucleotide typing (spoligotyping) for 43 spacers of the direct repeat
region (9 … performed using a kit according to the manufacturer’s instructions (Isogen/Omicium …
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[HTML] Supervised learning for the automated transcription of spacer classification from spoligotype films
D Jeffries, N Abernethy… – BMC bioinformatics, 2009 – biomedcentral.com
… a standardized method [4] using commercially prepared membranes from Isogen Biosciences
(Maarsen … Crawford JT: Transfer of a Mycobacterium tuberculosis genotyping method,
Spoligotyping, from a … AR, Tanaka MM: spolTools: online utilities for analyzing spoligotypes of …
Cited by 3 – Related articles – Cached – All 10 versions – Import into BibTeX
Y Chen, Y Chao, Q Deng, T Liu, J Xiang, J Chen… – Tuberculosis, 2009 – Elsevier
… Spoligotyping was performed to define the genotype of Beijing family strains with a
commercial kit (Isogen Bioscience BV, Maarssen, The Netherlands) according to the
manufacturer’s instruction. The spoligotype that only contained …
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[PDF] Tuberculosis Control in Oman
A Al-Maniri – 2009 – diss.kib.ki.se
… Spoligotyping of 312 isolates of Mycobacterium tuberculosis from TB patients
diagnosed during 2005-2007 was carried out in study IV. … 25 6.5 Study IV: Biodiversity
and clustering of M.tuberculosis isolates using spoligotyping ….. …
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N Motamedi – 2009 – edoc.ub.uni-muenchen.de
… modernen und der ursprünglichen Form von M. tuberculosis. Van Embden et al. 68
untersuchten M. canetti mit einem erweiterten Spoligotyping (siehe unten), das mehr als
die 43 Spacer der etablierten Technik aufsucht. Hier fielen Spacer auf, …
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[HTML] Tuberculosis disparity between US-born blacks and whites, Houston, Texas, USA
JA Serpa, LD Teeter, JM Musser… – Emerging infectious …, 2009 – ncbi.nlm.nih.gov
… isolates underwent susceptibility and genotype testing (insertion sequence 6110 restriction
fragment length polymorphism, spoligotyping, and genetic … the spacer oligonucleotide type
(spoligotype) was determined by using a commercially available kit (Isogen Bioscience BV …
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Molecular characterization of Mycobacterium Bovis isolates from selected slaughter houses in Kampala
J Asiimwe – 2009 – dspace.mak.ac.ug
… Spoligotyping revealed that three of the M. bovis isolates had spoligopatterns that had previously
been reported in Uganda while the seven … Although a few clusters were identified on analysis
of the spoligotypes and RFLP patterns, no geographical sub-structuring was observed …
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Predictors of poor treatment outcome in multi-and extensively drug-resistant pulmonary TB
K Kliiman… – European Respiratory Journal, 2009 – ersj.org.uk
… The strains were spoligotyped as described by Kamerbeek et al. 17 using commercially available
membranes (Isogen, Maarssen, The Netherlands) 17. … Mycobacterium tuberculosis complex genetic
diversity: mining the fourth international spoligotyping database (SpolDB4) for …
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[HTML] Tuberculosis Disparity between US-born Blacks and Whites, Houston, Texas, USA
F Figures, I Capitalization, AS Guides… – Tuberculosis, 2009 – wwwnc.cdc.gov
… isolates underwent susceptibility and genotype testing (insertion sequence 6110 restriction
fragment length polymorphism, spoligotyping, and genetic … the spacer oligonucleotide type
(spoligotype) was determined by using a commercially available kit (Isogen Bioscience BV …
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Molecular epidemiology of Mycobacterium tuberculosis in the United Arab Emirates (UAE)
A Saaidi… – 2009 – qmro.qmul.ac.uk
… HYPERLINK \l “_Toc237239861” **3.2.11 Identification of the UAE isolates by spoligotyping *
PAGEREF _Toc237239861 \h **133**. * HYPERLINK \l “_Toc237239862” **3.2.12 Comparison
between MIRU-typed and spoligotyped families in association with katG463/gyrA95 …
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[PDF] EPIDEMIOLOGÍA MOLECULAR DE LA
TEN ALMERÍA – hera.ugr.es
Page 1. FACULTAD DE MEDICINA UNIVERSIDAD DE GRANADA DEPARTAMENTO
DE MEDICINA TÍTULO TESIS DOCTORAL: EPIDEMIOLOGÍA MOLECULAR DE LA
TUBERCULOSIS EN ALMERÍA Page 2. Editor: Editorial …
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2008
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PC Chuang, H Liu, C Sola, YMA Chen… – Infection, Genetics and …, 2008 – Elsevier
… A commercially available spoligotyping kit (Isogen Bioscience BV, Maarssen, The Netherlands)
was used as … All 138 isolates were successfully genotyped by spoligotyping, but only 122 were
of … A total of 27 spoligotypes were identified; 124 isolates (89.9%, 124/138) were …
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E Augustynowicz-Kopeć, T Jagielski… – Journal of clinical …, 2008 – Am Soc Microbiol
… Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen,
The Netherlands), according to the manufacturer’s instructions and as … All spoligotypes obtained
were compared to those in the world spoligotyping database (SpolDB4) at …
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B Asiimwe, S Ghebremichael… – BMC infectious …, 2008 – biomedcentral.com
… The obtained signatures were compared in binary format with the international spoligotyping
database of the Pasteur Institute of … Spoligotypes. To determine the strain lineages present in the
sample, the 344 isolates were spoligotyped and binary outcomes compared with those …
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P Sharma, DS Chauhan, P Upadhyay… – Infection, Genetics and …, 2008 – Elsevier
… 2.3. Spoligotyping. Spoligotyping to detect 43 spacers was done with the help of commercially
available kit (Isogen Biosciences, BV, Maarsen, The Netherlands). … 3.1. Spoligotyping. A total of
46 distinct spoligotypes were obtained from this set of 97 M. tuberculosis isolates. …
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JP Mathuria, P Sharma, P Prakash… – Infection, Genetics and …, 2008 – Elsevier
… spacers by using the standard method (Kamerbeek et al., 1997) with the help of commercially
available kit (Isogen Biosciences, BV … Spoligotyping. … Presence of 19 orphan isolates indicates
the need of conducting multicentric studies to know all the prevalent spoligotypes in Indian …
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F Al-Otaibi… – pjms.com.pk
… Spoligotyping was performed with a commer- cially available kit (Isogen Bioscience).6,7 RESULTS …
Culturing of phosphate buffer in MGIT tube was positive for MTB after seven-day inoculation. Ten
different spoligotypes were identified among the 47 MTB isolates. …
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B Romero, A Aranaz, Á Sandoval, J Álvarez… – Veterinary …, 2008 – Elsevier
… detected by hybridization of the biotin-labelled PCR product onto spoligotyping membrane (Isogen
Bioscience BV … The discriminatory capacity of spoligotyping was h = 0.38 … The spoligotypes SB0950,
SB1230 and SB1232 were shared between domestic and wildlife animals (red …
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Spoligotyping of Mycobacterium tuberculosis in anthracotic bronchitis
K Najafizadeh, F Ghorbani, P Farnia… – … of Tuberculosis and …, 2008 – ingentaconnect.com
… use at −20°C. Spoligotyping Spoligotyping was performed, as previously described
by Kamerbeek et al.,10 with a commercially available kit (Isogen Bioscience BV,
Maarssen, The Netherlands). Analysis Information about the …
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Molecular analysis of drug‐resistant Mycobacterium tuberculosis isolates collected in central Poland
E Augustynowicz‐Kopeć, T Jagielski… – Clinical …, 2008 – Wiley Online Library
… DNA was prepared by the cetyl-trimethyl-ammonium bromide method [4]. Spoligotyping was
performed using a commercially available kit (Isogen Bioscience BV … was also performed as
described previously [2]. Isolates with indistinguishable spoligotypes and IS6110 …
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[HTML] Human tuberculosis caused by Mycobacterium bovis, Taiwan
R Jou, WL Huang… – Emerging infectious diseases, 2008 – ncbi.nlm.nih.gov
… Currently, 119 M. bovis spoligotypes are contained in the fourth international spoligotyping
database (SpolDB 4) and … strains, presence or absence of region of difference 1 was analyzed
(5). Spoligotyping was performed with a commercial kit (Isogen Bioscience BV …
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M Alonso, S Borrell, MM Lirola, E Bouza… – Tuberculosis, 2008 – Elsevier
… Spoligotyping was performed using primers of the spoligotyping kit (Isogen, Netherlands) and
following the manufacturer’s instructions. The spoligotypes were analyzed in the MIRU-VNTRplus
application (www.miru-vntrplus.org) 19 in order to identify the genetic lineages …
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Characterization of extensively drug-resistant Mycobacterium tuberculosis clinical isolates in China
Z Sun, Y Chao, X Zhang, J Zhang… – Journal of clinical …, 2008 – Am Soc Microbiol
… repeat (MIRU-VNTR) analysis. Spoligotyping was performed with a commercially
available kit (Isogen Bioscience BV, Maarssen, The Netherlands) according to the
manufacturer’s protocols. PCR-based MIRU-VNTR analysis …
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PC Hill, DJ Jackson-Sillah, A Fox, RH Brookes… – PLoS One, 2008 – dx.plos.org
… For molecular subtyping of index case isolates, we extracted mycobacterial DNA using CTAB
and chloroform[18]. We performed spoligotyping using membranes from Isogen Biosciences
(http:/www.isogen-lifescience.com), as previously described[19]. …
Cited by 85 – Related articles – Cached – All 15 versions – Import into BibTeX
[PDF] : Transmission from South American Sea Lion () to Bactrian Camel () and Malayan Tapirs ()
I Moser, WM Prodinger, H Hotzel… – Veterinary …, 2008 – peer.ccsd.cnrs.fr
… by 30 cycles of 96°C for 15 sec, 67°C for 60 sec, 72°C for 60 sec, and a final extension at 18
72°C 300 sec. 19 Spoligotyping. Spoligotyping (9; Isogen Bioscience, The Netherlands) was
performed using 20 DNA extracted from cultures. 21 MIRU typing. …
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M Ashworth, KL Horan, R Freeman… – Journal of clinical …, 2008 – Am Soc Microbiol
… Only one isolate per case was included in the analysis. Genotyping.Spoligotyping
was performed according to previously published methods by using a spoligotyping
kit (Isogen, IJsselstein, The Netherlands) (12). MIRU analysis …
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J Oloya, J Opuda-Asibo, R Kazwala… – Epidemiology and …, 2008 – Cambridge Univ Press
… MTC were submitted for further differentiation by the spoligotyping kit ac- cording to the
manufacturer’s instructions (Isogen, Life Science … One of the spoligotypes (Hu-4 and Hu-5 in Fig.
1) is labelled LAM 3/S in the spoligotyping database SpolDB4, and has been isolated in 118 …
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Genotypic diversity of extensively drug-resistant tuberculosis (XDR-TB) in South Africa
CK Mlambo, RM Warren, X Poswa… – … of tuberculosis and …, 2008 – ingentaconnect.com
… Spoligotyping was performed ac- cording to the internationally standardised protocol11 using
commercially … of the genotyping data, serial isolates from 13 patients were randomly sampled
and spoligotyped. … was used to assign geno- type families to the spoligotypes obtained.12 …
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[HTML] Molecular epidemiology and evolutionary genetics of Mycobacterium tuberculosis in Taipei
HY Dou, FC Tseng, CW Lin, JR Chang… – BMC infectious …, 2008 – biomedcentral.com
… Of 356 isolates spoligotyped, 290 (81.4%) displayed known spoligotypes and 66 were not …
Spoligotyping was carried out according to the manufacturer’s instructions (Isogen Bioscience
BV … The resulting spoligotypes were documented under a binary code representing either a …
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[HTML] Genotyping and drug resistance patterns of M. tuberculosis strains in Pakistan
M Tanveer, Z Hasan, A Siddiqui, A Ali… – BMC Infectious …, 2008 – biomedcentral.com
… Spoligotyping of M. tuberculosis isolates. All M. tuberculosis strains were spoligotyped and their
data analyzed using UPGMA calculation in the Bionumerics software. … one (78%) isolates grouped
into 59 different “shared types” while 205 (22%) isolates had ‘orphan’ spoligotypes. …
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I Moser, WM Prodinger, H Hotzel, R Greenwald… – Veterinary …, 2008 – Elsevier
… 2.5. Spoligotyping. Spoligotyping (Kamerbeek et al., 1997; Isogen Bioscience, The Netherlands)
was performed using DNA extracted from cultures. 2.6. MIRU typing. MIRU typing was
performed targeting 12 chromosomal loci as given by Mazars et al. …
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Mycobacterium tuberculosis population structures differ significantly on two Indonesian Islands
I Parwati, R Van Crevel, M Sudiro… – Journal of clinical …, 2008 – Am Soc Microbiol
… Therefore, we have spoligotyped M. tuberculosis isolates from a large cohort of patients …
Mycobacterium tuberculosis complex genetic diversity: mining the fourth international
spoligotyping database (SpolDB4) for … Global distribution of Mycobacterium tuberculosis …
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Development of multiplex assay for rapid characterization of Mycobacterium tuberculosis
IL Bergval, R Vijzelaar, ER Dalla Costa… – Journal of clinical …, 2008 – Am Soc Microbiol
… Strains 1 to 29 were selected for their specific spoligotypes and were used to determine the
correlation between MLPA, the spoligotype, and the sequence of ogt codon 15 (a marker for the
M … Spoligotyping.Mycobacterium tuberculosis strains were spoligotyped by using a …
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Mycobacterium bovis in wildlife in France
G Zanella, B Durand, J Hars… – Journal of wildlife …, 2008 – Wildlife Dis Assoc
… Biotin-labeled PCR products were detected on a spoligotyping membrane (Isogen Bioscience BV,
Maarssen, The Netherlands). Purified sterile water and M. bovis and M. tuberculosis controls
included in the spoligomembrane kit were included in each batch of tests. …
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T Prammananan, W Cheunoy… – Clinical …, 2008 – Wiley Online Library
… In contrast, of 112 rifampicin-susceptible isolates that were spoligotyped, only 51 (45.5%) were
revealed to be … a One additional MDRTB isolate was not available for spoligotyping. … Association
of specific mutations in katG, rpoB, rpsL and rrs genes with spoligotypes of multidrug …
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US Rajapaksa, TC Victor, AJ Perera… – Transactions of the …, 2008 – Elsevier
… Spoligotyping was performed using a commercially available kit (Isogen Bioscience BV, Maarssen,
The … Spoligotyping of 98 M. tuberculosis isolates revealed a total of 43 patterns, with six … The finding
of identical spoligotypes in geographically widely separated countries may be …
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F Doustdar, AD Khosravi, P Farnia, AR Bahrmand… – Tanaffos, 2008 – nritld.ac.ir
… tuberculosis isolates are defined on the basis of shared spoligotypes or closely related IS6110 …
Spoligotyping was performed with a commercially available kit to detect 43 known spacers … DR
locus according to the instructions supplied by the manufacturer (Isogen Bioscience BV …
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Molecular epidemiology of Mycobacterium bovis: usefulness in international trade
F Milian-Suazo, B Harris, CA Díaz… – Preventive veterinary …, 2008 – Elsevier
… Spoligotyping was performed following the protocol described by Kamerbeek et al … Eighty-four
M. bovis strains from Mexico and the US were spoligotyped under the same protocol in two … When
comparing spoligotypes from the two laboratories, 50 (59%) out of the 84 strains were …
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Attempts to revive Mycobacterium tuberculosis from 300‐year‐old human mummies
E Lemma, O Zimhony, CL Greenblatt… – FEMS microbiology …, 2008 – Wiley Online Library
… Spoligotyping was performed at day 250 following the instructions of the manufacturer
of the ISOGEN Life Science Spoligotyping kit. Animal inoculations. Two sets of six
female guinea pigs weighing an average of 250 g were used. …
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Mycobacterium tuberculosis Uganda genotype is the predominant cause of TB in Kampala, Uganda
BB Asiimwe, T Koivula, G Kallenius… – … of Tuberculosis and …, 2008 – ingentaconnect.com
… Data analysis Spoligotypes were analysed using BioNumerics soft- ware (version 4.6, Applied
Maths, Kortrijk, Belgium) as a character type. … Spoligotyping To determine the predominant strain
lineages, the 344 isolates were spoligotyped and binary outcomes compared with …
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Pyrazinamide resistance among South African multidrug-resistant Mycobacterium tuberculosis isolates
M Mphahlele, H Syre, H Valvatne… – Journal of clinical …, 2008 – Am Soc Microbiol
… (i) Spoligotyping.Spoligotyping was performed to further … The resulting amplification products were
then hybridized to a commercially available membrane (Isogen Bioscience BV, Bilthoven, The
Netherlands), with covalently linked parallel rows of 43 synthetic oligonucleotides …
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LR Martinez, B Harris, WC Black… – Journal of Veterinary …, 2008 – vdi.sagepub.com
… The 6 isolates that comprised MLVA type N were divided into 3 spoligotypes: 1 isolate into SP2 …
A larger database of MLVA typed isolates, in combination with spoligotyping and conventional
epidemiological investigations, can provide a … n. Spacer sequences, Isogen Life Science …
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[CITATION] First Insight Into Mycobacterium tuberculosis Biodiversity in Sri Lanka: Evidence for Predominance of Beijing Genotype and Ancestral Lineages Among …
US Rajapaksa, AJ Perera, TC Victor… – International Journal of …, 2008 – Elsevier
[BOOK] Molecular characterization of Mycobacterium tuberculosis complex from Kampala, Uganda
B Asiimwe – 2008 – dspace.mak.ac.ug
… and colleagues (69), which is commercially available as a kit (Isogen Bioscience BV … the important
lineages and sub-lineages in SpolDB4.0, the international spoligotyping database iii … To investigate
the association between the predominant spoligotypes, HIV sero- status and anti …
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[PDF] A tuberkulózis molekuláris epidemiológiai vonatkozásai Magyarországon
L Judit – phd.sote.hu
Page 1. A tuberkulózis molekuláris epidemiológiai vonatkozásai Magyarországon
Doktori értekezés Lukács Judit Semmelweis Egyetem Klinikai Orvostudományok
Doktori Iskola Témavezetı: Dr. Somoskövi Ákos, MTA doktora …
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Y Casart, L Turcios, I Florez, R Jaspe, E Guerrero… – Tuberculosis, 2008 – Elsevier
… DR locus was carried out as described earlier, 15 with membranes supplied by Isogen Bioscience
BV … to mention that all the clones isolated from 1C8 or 1D3 displayed identical spoligotyping and
MIRU … A1 insertion 12 (Figure 1B), (ii) The IS6110-RFLPs and spoligotypes did not …
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Intérêt du typage moléculaire pour l’épidémiologie de la tuberculose en Limousin (1998–2006)
H Bezanahary, MC Baclet, C Sola… – Médecine et Maladies …, 2008 – Elsevier
… Keywords: MIRU15 typing; Mycobacterium tuberculosis; Spoligotyping. … L’hybridation (1H à 60
°C) des amplicons a été réalisée sur une membrane Isogen ® (Bioscience BV … sous forme d’un
code octal et comparés aux données de la banque de spoligotypes SpolDB4 (Institut …
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[PDF] TABLA 8.1 _ GEL DE CORRIMIENTO
TNN – Identificación y diagnóstico de Actinomicetales … – saber.ula.ve
… caontb. rivm. nl/Manuals/principl. html. Por su parte, Isogen Biosciences (http://www. isogen.
nl) ofrece membranas con ADN inmobilizado de espaciadores, para utilizarse en pruebas
de spoligotyping específicas para cepas de M. bovis/M. tuberculosis. …
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CLS Mucavele – 2008 – repository.utl.pt
… number of spoligotypes were identical to profiles found in the region. The data reported showed
that migration and HIV might be factors of dissemination of TB in Humans in Mozambique. Factors
that can compromise the performance of Spoligotyping technique and other …
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[BOOK] Molecular Characterisation of Antibiotic Resistance in Mycobacterium Tuberculosis
P Juréen – 2008 – diss.kib.ki.se
… personnel (91). In order to overcome this, spoligotyping was evolved. This … repeat) (91).
MIRU-VNTR analyses copy-numbers of repeats, however, in contrast to spoligotyping,
these have several sites scattered around the genome. Since …
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2007
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J Chen, AG Tsolaki, X Shen, X Jiang, J Mei… – Tuberculosis, 2007 – Elsevier
… Spoligotyping was performed by using an Isogen Bioscience spoligotyping kit (Isogen Life Science …
Figure 3. Beijing/W Spoligotypes observed in 182 isolates with deletion RD105. Comparison
of the multiplex PCR with spoligotyping revealed 100% sensitivity and specificity for …
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S David, DR Ribeiro, A Antunes, C Portugal… – Infection, Genetics and …, 2007 – Elsevier
… Spoligotyping was carried out according to the manufacturer’s instructions (Isogen Bioscience
BV, Maarsen, Netherlands). The resulting spoligotypes were documented under a binary code
representing either a positive or negative hybridization result (n and o) and analyzed …
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N Suresh, J Arora, H Pant, T Rana… – Journal of microbiological …, 2007 – Elsevier
… available kit, according to the instructions supplied by the manufacturer (Isogen Bioscience, BV …
a positive signal in spoligotyping, thus demonstrating the efficiency of spoligotyping in a … analysis
of DNA extracted from stored ZN slides identified tuberculosis spoligotypes in 41 …
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MT Javed, A Aranaz, L De Juan, J Bezos… – Tuberculosis, 2007 – Elsevier
… using two membranes: a commercial membrane containing the 43 spacers described earlier
23 (Isogen Bioscience BV … Number of spoligotyping patterns of M. bovis and M. caprae.
Thirty-one different spoligotypes were identified among the 308